SRX23813295 GSM8123065_r1 SRP492912 PRJNA1082798 SRS20635906 GSM8123065 GSM8123065 ATAC-seq GENOMIC other Briefly, 50,000 cells per condition were washed in cold PBS and resuspended in 50 μl of cold lysis buffer. Samples were centrifuged for 10 min at 500g, 4 °C and the cell pellet resuspended in the transposition reaction mix and incubated at 37 °C for 30 min. Samples were purified using the Qiagen MinElute PCR Purification Kit. Transposed DNA was eluted in 10 μl of elution buffer,and subjected to PCR amplification using barcoded primers and NEBNext High Fidelity PCR Master Mix. ATAC-seq libraries were purified using 2× volumes of AMPure XP beads to remove fragments below 100 bp. Library quality was assessed using a Bioanalyser High Sensitivity DNA Analysis Kit Illumina HiSeq 2000 SRX23813296 GSM8123066_r1 SRP492912 PRJNA1082798 SRS20635904 GSM8123066 GSM8123066 ATAC-seq GENOMIC other Briefly, 50,000 cells per condition were washed in cold PBS and resuspended in 50 μl of cold lysis buffer. Samples were centrifuged for 10 min at 500g, 4 °C and the cell pellet resuspended in the transposition reaction mix and incubated at 37 °C for 30 min. Samples were purified using the Qiagen MinElute PCR Purification Kit. Transposed DNA was eluted in 10 μl of elution buffer,and subjected to PCR amplification using barcoded primers and NEBNext High Fidelity PCR Master Mix. ATAC-seq libraries were purified using 2× volumes of AMPure XP beads to remove fragments below 100 bp. Library quality was assessed using a Bioanalyser High Sensitivity DNA Analysis Kit Illumina HiSeq 2000 SRX23813297 GSM8123067_r1 SRP492912 PRJNA1082798 SRS20635905 GSM8123067 GSM8123067 ATAC-seq GENOMIC other Briefly, 50,000 cells per condition were washed in cold PBS and resuspended in 50 μl of cold lysis buffer. Samples were centrifuged for 10 min at 500g, 4 °C and the cell pellet resuspended in the transposition reaction mix and incubated at 37 °C for 30 min. Samples were purified using the Qiagen MinElute PCR Purification Kit. Transposed DNA was eluted in 10 μl of elution buffer,and subjected to PCR amplification using barcoded primers and NEBNext High Fidelity PCR Master Mix. ATAC-seq libraries were purified using 2× volumes of AMPure XP beads to remove fragments below 100 bp. Library quality was assessed using a Bioanalyser High Sensitivity DNA Analysis Kit Illumina HiSeq 2000 SRX23813298 GSM8123068_r1 SRP492912 PRJNA1082798 SRS20635907 GSM8123068 GSM8123068 ATAC-seq GENOMIC other Briefly, 50,000 cells per condition were washed in cold PBS and resuspended in 50 μl of cold lysis buffer. Samples were centrifuged for 10 min at 500g, 4 °C and the cell pellet resuspended in the transposition reaction mix and incubated at 37 °C for 30 min. Samples were purified using the Qiagen MinElute PCR Purification Kit. Transposed DNA was eluted in 10 μl of elution buffer,and subjected to PCR amplification using barcoded primers and NEBNext High Fidelity PCR Master Mix. ATAC-seq libraries were purified using 2× volumes of AMPure XP beads to remove fragments below 100 bp. Library quality was assessed using a Bioanalyser High Sensitivity DNA Analysis Kit Illumina HiSeq 2000 SRX23813299 GSM8123069_r1 SRP492912 PRJNA1082798 SRS20635908 GSM8123069 GSM8123069 ATAC-seq GENOMIC other Briefly, 50,000 cells per condition were washed in cold PBS and resuspended in 50 μl of cold lysis buffer. Samples were centrifuged for 10 min at 500g, 4 °C and the cell pellet resuspended in the transposition reaction mix and incubated at 37 °C for 30 min. Samples were purified using the Qiagen MinElute PCR Purification Kit. Transposed DNA was eluted in 10 μl of elution buffer,and subjected to PCR amplification using barcoded primers and NEBNext High Fidelity PCR Master Mix. ATAC-seq libraries were purified using 2× volumes of AMPure XP beads to remove fragments below 100 bp. Library quality was assessed using a Bioanalyser High Sensitivity DNA Analysis Kit Illumina HiSeq 2000 SRX23813300 GSM8123070_r1 SRP492912 PRJNA1082798 SRS20635909 GSM8123070 GSM8123070 ATAC-seq GENOMIC other Briefly, 50,000 cells per condition were washed in cold PBS and resuspended in 50 μl of cold lysis buffer. Samples were centrifuged for 10 min at 500g, 4 °C and the cell pellet resuspended in the transposition reaction mix and incubated at 37 °C for 30 min. Samples were purified using the Qiagen MinElute PCR Purification Kit. Transposed DNA was eluted in 10 μl of elution buffer,and subjected to PCR amplification using barcoded primers and NEBNext High Fidelity PCR Master Mix. ATAC-seq libraries were purified using 2× volumes of AMPure XP beads to remove fragments below 100 bp. Library quality was assessed using a Bioanalyser High Sensitivity DNA Analysis Kit Illumina HiSeq 2000