SRX23851600 GSM8128797_r1 SRP493622 PRJNA1084743 SRS20671672 GSM8128797 GSM8128797 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000 SRX23851601 GSM8128798_r1 SRP493622 PRJNA1084743 SRS20671673 GSM8128798 GSM8128798 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000 SRX23851602 GSM8128799_r1 SRP493622 PRJNA1084743 SRS20671674 GSM8128799 GSM8128799 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000 SRX23851603 GSM8128800_r1 SRP493622 PRJNA1084743 SRS20671675 GSM8128800 GSM8128800 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000 SRX23851604 GSM8128801_r1 SRP493622 PRJNA1084743 SRS20671676 GSM8128801 GSM8128801 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000 SRX23851605 GSM8128802_r1 SRP493622 PRJNA1084743 SRS20671677 GSM8128802 GSM8128802 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000 SRX23851606 GSM8128803_r1 SRP493622 PRJNA1084743 SRS20671678 GSM8128803 GSM8128803 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000 SRX23851607 GSM8128804_r1 SRP493622 PRJNA1084743 SRS20671679 GSM8128804 GSM8128804 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000 SRX23851608 GSM8128805_r1 SRP493622 PRJNA1084743 SRS20671680 GSM8128805 GSM8128805 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000 SRX23851609 GSM8128806_r1 SRP493622 PRJNA1084743 SRS20671681 GSM8128806 GSM8128806 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000 SRX23851610 GSM8128807_r1 SRP493622 PRJNA1084743 SRS20671682 GSM8128807 GSM8128807 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000 SRX23851611 GSM8128808_r1 SRP493622 PRJNA1084743 SRS20671683 GSM8128808 GSM8128808 ATAC-seq TRANSCRIPTOMIC other ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates. Illumina NovaSeq 6000