SRX23867629 GSM8134124_r1 SRP469972 PRJNA1035108 SRS20686577 GSM8134124 GSM8134124 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA on day12 were chopped into small pieces (2-4 mm size) with a scalpel before dissociation with Miltenyi's gentle MACS enzyme mix (mouse tumor dissociation kit, # 130-096-730) in a Miltenyi GentleMACS Octo Dissociator (Miltenyi Biotec Inc., San Diego, CA) with pre- set 37C_mTDK_1 protocol. After dissociation, the cell suspension was filtered through 70uM MACS smart strainer and dead cells were removed using dead cell removal kit (Miltenyi Biotec Inc.,). Cells were counted using Countess II Automated Cell Counter (Thermo Fisher Scientific) and hemocytometer for cell concentration and viability using Trypan Blue stain 0.4% (Invitrogen). Viability of cells was above 90% and 10X genomics recommendended cells were used to target about 10,000 cells. Single cell gene expression libraries were created using Chromium Next GEM Single Cell 3' (v3.1 Chemistry) (10x Genomics), Chromium Next GEM Chip G Single Cell Kit (10x Genomics), and Single Index Kit T Set A (10x Genomics) according to the manufacturer's instructions. Illumina NovaSeq 6000 SRX23867630 GSM8134125_r1 SRP469972 PRJNA1035108 SRS20686584 GSM8134125 GSM8134125 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA on day12 were chopped into small pieces (2-4 mm size) with a scalpel before dissociation with Miltenyi's gentle MACS enzyme mix (mouse tumor dissociation kit, # 130-096-730) in a Miltenyi GentleMACS Octo Dissociator (Miltenyi Biotec Inc., San Diego, CA) with pre- set 37C_mTDK_1 protocol. After dissociation, the cell suspension was filtered through 70uM MACS smart strainer and dead cells were removed using dead cell removal kit (Miltenyi Biotec Inc.,). Cells were counted using Countess II Automated Cell Counter (Thermo Fisher Scientific) and hemocytometer for cell concentration and viability using Trypan Blue stain 0.4% (Invitrogen). Viability of cells was above 90% and 10X genomics recommendended cells were used to target about 10,000 cells. Single cell gene expression libraries were created using Chromium Next GEM Single Cell 3' (v3.1 Chemistry) (10x Genomics), Chromium Next GEM Chip G Single Cell Kit (10x Genomics), and Single Index Kit T Set A (10x Genomics) according to the manufacturer's instructions. Illumina NovaSeq 6000 SRX23867631 GSM8134126_r1 SRP469972 PRJNA1035108 SRS20686583 GSM8134126 GSM8134126 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA on day12 were chopped into small pieces (2-4 mm size) with a scalpel before dissociation with Miltenyi's gentle MACS enzyme mix (mouse tumor dissociation kit, # 130-096-730) in a Miltenyi GentleMACS Octo Dissociator (Miltenyi Biotec Inc., San Diego, CA) with pre- set 37C_mTDK_1 protocol. After dissociation, the cell suspension was filtered through 70uM MACS smart strainer and dead cells were removed using dead cell removal kit (Miltenyi Biotec Inc.,). Cells were counted using Countess II Automated Cell Counter (Thermo Fisher Scientific) and hemocytometer for cell concentration and viability using Trypan Blue stain 0.4% (Invitrogen). Viability of cells was above 90% and 10X genomics recommendended cells were used to target about 10,000 cells. Single cell gene expression libraries were created using Chromium Next GEM Single Cell 3' (v3.1 Chemistry) (10x Genomics), Chromium Next GEM Chip G Single Cell Kit (10x Genomics), and Single Index Kit T Set A (10x Genomics) according to the manufacturer's instructions. Illumina NovaSeq 6000 SRX23867632 GSM8134127_r1 SRP469972 PRJNA1035108 SRS20686582 GSM8134127 GSM8134127 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA on day12 were chopped into small pieces (2-4 mm size) with a scalpel before dissociation with Miltenyi's gentle MACS enzyme mix (mouse tumor dissociation kit, # 130-096-730) in a Miltenyi GentleMACS Octo Dissociator (Miltenyi Biotec Inc., San Diego, CA) with pre- set 37C_mTDK_1 protocol. After dissociation, the cell suspension was filtered through 70uM MACS smart strainer and dead cells were removed using dead cell removal kit (Miltenyi Biotec Inc.,). Cells were counted using Countess II Automated Cell Counter (Thermo Fisher Scientific) and hemocytometer for cell concentration and viability using Trypan Blue stain 0.4% (Invitrogen). Viability of cells was above 90% and 10X genomics recommendended cells were used to target about 10,000 cells. Single cell gene expression libraries were created using Chromium Next GEM Single Cell 3' (v3.1 Chemistry) (10x Genomics), Chromium Next GEM Chip G Single Cell Kit (10x Genomics), and Single Index Kit T Set A (10x Genomics) according to the manufacturer's instructions. Illumina NovaSeq 6000 SRX23867633 GSM8134128_r1 SRP469972 PRJNA1035108 SRS20686578 GSM8134128 GSM8134128 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA on day12 were chopped into small pieces (2-4 mm size) with a scalpel before dissociation with Miltenyi's gentle MACS enzyme mix (mouse tumor dissociation kit, # 130-096-730) in a Miltenyi GentleMACS Octo Dissociator (Miltenyi Biotec Inc., San Diego, CA) with pre- set 37C_mTDK_1 protocol. After dissociation, the cell suspension was filtered through 70uM MACS smart strainer and dead cells were removed using dead cell removal kit (Miltenyi Biotec Inc.,). Cells were counted using Countess II Automated Cell Counter (Thermo Fisher Scientific) and hemocytometer for cell concentration and viability using Trypan Blue stain 0.4% (Invitrogen). Viability of cells was above 90% and 10X genomics recommendended cells were used to target about 10,000 cells. Single cell gene expression libraries were created using Chromium Next GEM Single Cell 3' (v3.1 Chemistry) (10x Genomics), Chromium Next GEM Chip G Single Cell Kit (10x Genomics), and Single Index Kit T Set A (10x Genomics) according to the manufacturer's instructions. Illumina NovaSeq 6000