Paired-end Illumina sequencing of C. botulinum PB84A isolated from peanut butter from retail survey

SRX23868033 MER-2021-007813 Paired-end Illumina sequencing of C. botulinum PB84A isolated from peanut butter from retail survey SRP493850 PRJNA1085258 Single colony grown 24h at 35 degrees in 25mL TPGY. Pellet resuspended in 1mL of gelatin phosphate buffer and 1mL of 2 Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with RNAse A treatment. NexteraXT library prep; paired-end (2x300) sequencing using MiSeq and v3 chemistry SRS20686925 SAMN40298541 MER-2021-007813 WGS GENOMIC RANDOM Illumina MiSeq SRX23868034 BMH-2021-000779 Paired-end Illumina sequencing of C. botulinum EF0611A enema fluid isolate from adult botulism case SRP493850 PRJNA1085258 Single colony grown 24h at 35 degrees in 25mL TPGY. Pellet resuspended in 1mL of gelatin phosphate buffer and 1mL of 2 Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with RNAse A treatment. NexteraXT library prep; paired-end (2x300) sequencing using MiSeq and v3 chemistry SRS20686926 SAMN40298542 BMH-2021-000779 WGS GENOMIC RANDOM Illumina MiSeq SRX23868035 BMH-2021-001129 Paired-end Illumina sequencing of C. botulinum PB0612A isolated from peanut butter from adult botulism case SRP493850 PRJNA1085258 Single colony grown 24h at 35 degrees in 25mL TPGY. Pellet resuspended in 1mL of gelatin phosphate buffer and 1mL of 2 Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with RNAse A treatment. NexteraXT library prep; paired-end (2x300) sequencing using MiSeq and v3 chemistry SRS20686918 SAMN40298543 BMH-2021-001129 WGS GENOMIC RANDOM Illumina MiSeq SRX23868036 MER-2021-007809 Paired-end Illumina sequencing of C. botulinum PB10507A isolated from peanut butter associated with adult botulism case SRP493850 PRJNA1085258 Single colony grown 24h at 35 degrees in 25mL TPGY. Pellet resuspended in 1mL of gelatin phosphate buffer and 1mL of 2 Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with RNAse A treatment. NexteraXT library prep; paired-end (2x300) sequencing using MiSeq and v3 chemistry SRS20686919 SAMN40298544 MER-2021-007809 WGS GENOMIC RANDOM Illumina MiSeq SRX23868037 BMH-2021-000789 Paired-end Illumina sequencing of C. botulinum PB20607A isolated from peanut butter associated with adult botulism case SRP493850 PRJNA1085258 Single colony grown 24h at 35 degrees in 25mL TPGY. Pellet resuspended in 1mL of gelatin phosphate buffer and 1mL of 2 Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with RNAse A treatment. NexteraXT library prep; paired-end (2x300) sequencing using MiSeq and v3 chemistry SRS20686915 SAMN40298545 BMH-2021-000789 WGS GENOMIC RANDOM Illumina MiSeq SRX23868038 BMH-2021-000791 Paired-end Illumina sequencing of C. botulinum PB30607A isolated from peanut butter associated with adult botulism case SRP493850 PRJNA1085258 Single colony grown 24h at 35 degrees in 25mL TPGY. Pellet resuspended in 1mL of gelatin phosphate buffer and 1mL of 2 Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with RNAse A treatment. NexteraXT library prep; paired-end (2x300) sequencing using MiSeq and v3 chemistry SRS20686924 SAMN40298546 BMH-2021-000791 WGS GENOMIC RANDOM Illumina MiSeq