SRP494046 PRJNA1085399 RNA-Seq data of Verticillium dahliae and Gossypium hirsutum treated by KRS027 Previously, an antagonistic strain KRS027 was isolated from the rhizosphere soil of a healthy plant found in a pathogen-infested cotton field from Xinjiang, China, and found that it can protect cotton against Verticillium wilt caused by Verticillium dahliae and promote plant growth. For further reveal the antagonistic and growth-promoting mechanism, we performed the RNA-Seq of Verticillium dahliae and Gossypium hirsutum treated by KRS027. These differentially expressed genes will provide new insights into the mechanism of action of KRS027.