SRX699035 CSD.20 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699074 SAMN03024520 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699036 CSD.22 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699075 SAMN03024521 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699037 CSD.24 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699076 SAMN03024522 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699038 CSD.26 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699077 SAMN03024523 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699039 MED.20 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699078 SAMN03024524 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699040 MED.22 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699079 SAMN03024525 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699041 MED.24 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699080 SAMN03024526 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699042 MED.26 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699081 SAMN03024527 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699043 PND.20 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699082 SAMN03024528 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699044 PND.22 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699083 SAMN03024529 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699045 PND.24 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699084 SAMN03024530 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699046 PND.26 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699085 SAMN03024531 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699047 CDD.20 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699086 SAMN03024532 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699048 CDD.22 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699087 SAMN03024533 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699049 CDD.24 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699088 SAMN03024534 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699050 CDD.26 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699089 SAMN03024535 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699051 SMD.20 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699090 SAMN03024536 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699052 SMD.22 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699091 SAMN03024537 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699053 SMD.24 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699092 SAMN03024538 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000 SRX699054 SMD.26 SRP046998 PRJNA260645 "Vitis vinifera L. cultivars Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyards. Selected blocks within our experimental vineyard were exposed to a moderate water deficit treatment during the 2012 growing and harvest season. Water deficit was assessed through weekly mid-day stem water potentials on fully expanded, mature leaves using a pressure chamber. Drip irrigation was initiated when stem water potentials of the vines reached their target treatment levels. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ??Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (??Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ??Brix level in 1?? increments from 19 - 26?? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen. Frozen skin tissue was ground thoroughly in a Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions. Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions. The goal of this research project was to profile transcriptional changes in the mature berry skins, following a season long water deficit treatment, as soluble sugars increase from 20 to 26 ??Brix, and to identify major players that affect fruit and ultimately wine quality. In this analysis, we also investigated how water deficit affects transcription in five cultivars. Each cultivar, treatment and Brix level was comprised of three biological replicates that were each sequenced. This work complements project PRJNA260535 (BioProject accession SRP046456), where we surveyed seven cultivars. " SRS699093 SAMN03024539 RNA-Seq TRANSCRIPTOMIC PolyA 50 0 Application Read Forward 1 Illumina HiSeq 2000