SRX700012 SM3076T5L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700297 SAMN03022938 SM3076T5L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700013 SM3076T5R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700297 SAMN03022938 SM3076T5R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700014 SM3062T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700298 SAMN03022927 SM3062T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700015 SM3062T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700300 SAMN03022928 SM3062T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700016 SM3062T3L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700299 SAMN03022929 SM3062T3L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700017 SM3062T3R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700299 SAMN03022929 SM3062T3R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700018 SM3073T3L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700301 SAMN03022930 SM3073T3L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700019 SM3073T5L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700303 SAMN03022931 SM3073T5L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700020 SM3073T7L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700302 SAMN03022932 SM3073T7L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700021 SM3073T7R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700302 SAMN03022932 SM3073T7R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700022 SM3073T8L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700304 SAMN03022933 SM3073T8L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700023 SM3073T8R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700304 SAMN03022933 SM3073T8R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700024 SM3074T3R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700305 SAMN03022934 SM3074T3R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700025 SM3074T5L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700306 SAMN03022935 SM3074T5L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700026 SM3074T5R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700306 SAMN03022935 SM3074T5R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700027 SM3076T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700307 SAMN03022936 SM3076T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700028 SM3076T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700307 SAMN03022936 SM3076T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700029 SM3076T4L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700308 SAMN03022937 SM3076T4L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700030 SM3076T6L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700309 SAMN03022939 SM3076T6L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700031 SM3076T6R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700309 SAMN03022939 SM3076T6R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700032 SM3084T3L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700310 SAMN03022940 SM3084T3L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700033 SM3084T3R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700310 SAMN03022940 SM3084T3R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700034 SM3151T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700311 SAMN03022941 SM3151T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700035 SM3151T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700311 SAMN03022941 SM3151T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700036 SM3156T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700312 SAMN03022942 SM3156T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700037 SM3156T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700312 SAMN03022942 SM3156T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700038 SM3156T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700313 SAMN03022943 SM3156T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700039 SM3156T3L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700314 SAMN03022944 SM3156T3L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700040 SM3156T3R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700314 SAMN03022944 SM3156T3R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700041 SM3156T4L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700315 SAMN03022945 SM3156T4L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700042 SM3158T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700316 SAMN03022946 SM3158T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700043 SM3158T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700316 SAMN03022946 SM3158T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700044 SM3158T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700317 SAMN03022947 SM3158T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700045 SM3158T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700317 SAMN03022947 SM3158T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700046 SM3159T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700318 SAMN03022948 SM3159T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700047 SM3159T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700318 SAMN03022948 SM3159T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700048 SM3159T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700319 SAMN03022949 SM3159T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700049 SM3159T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700319 SAMN03022949 SM3159T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700050 SM3161T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700320 SAMN03022950 SM3161T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700051 SM3161T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700320 SAMN03022950 SM3161T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700052 SM3161T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700321 SAMN03022951 SM3161T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700053 SM3161T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700321 SAMN03022951 SM3161T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700054 SM3161T3L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700322 SAMN03022952 SM3161T3L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700055 SM3161T3R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700322 SAMN03022952 SM3161T3R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700056 SM3161T4L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700323 SAMN03022953 SM3161T4L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700057 SM3161T4R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700323 SAMN03022953 SM3161T4R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700058 SM3161T5L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700324 SAMN03022954 SM3161T5L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700059 SM3161T5R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700324 SAMN03022954 SM3161T5R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700060 SM3164T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700325 SAMN03022955 SM3164T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700061 SM3164T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700325 SAMN03022955 SM3164T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700062 SM3164T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700326 SAMN03022956 SM3164T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700063 SM3164T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700326 SAMN03022956 SM3164T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700064 SM3164T3L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700327 SAMN03022957 SM3164T3L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700065 SM3164T3R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700327 SAMN03022957 SM3164T3R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700066 SM3165T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700328 SAMN03022958 SM3165T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700067 SM3165T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700328 SAMN03022958 SM3165T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700068 SM3165T3L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700329 SAMN03022959 SM3165T3L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700069 SM3165T3R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700329 SAMN03022959 SM3165T3R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700070 SM3253T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700330 SAMN03022960 SM3253T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700071 SM3253T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700330 SAMN03022960 SM3253T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700072 SM3253T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700331 SAMN03022961 SM3253T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700073 SM3253T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700331 SAMN03022961 SM3253T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700074 SM3253T6L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700332 SAMN03022962 SM3253T6L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700075 SM3253T6R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700332 SAMN03022962 SM3253T6R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700076 SM3253T7L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700333 SAMN03022963 SM3253T7L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700077 SM3253T7R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700333 SAMN03022963 SM3253T7R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700078 SM3259T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700334 SAMN03022964 SM3259T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700079 SM3259T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700334 SAMN03022964 SM3259T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700080 SM3259T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700335 SAMN03022965 SM3259T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700081 SM3259T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700335 SAMN03022965 SM3259T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700082 SM3259T4L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700336 SAMN03022966 SM3259T4L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700083 SM3259T4R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700336 SAMN03022966 SM3259T4R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700084 SM3259T5L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700337 SAMN03022967 SM3259T5L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700085 SM3259T5R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700337 SAMN03022967 SM3259T5R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700086 SM3284T6L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700338 SAMN03022968 SM3284T6L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700087 SM3284T6R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700338 SAMN03022968 SM3284T6R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700088 SM3297T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700339 SAMN03022969 SM3297T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700089 SM3297T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700339 SAMN03022969 SM3297T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700090 SM3297T3L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700340 SAMN03022970 SM3297T3L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700091 SM3297T3R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700340 SAMN03022970 SM3297T3R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700092 SM3297T4L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700341 SAMN03022971 SM3297T4L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700093 SM3297T4R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700341 SAMN03022971 SM3297T4R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700094 SM3297T5L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700342 SAMN03022972 SM3297T5L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700095 SM3297T5R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700342 SAMN03022972 SM3297T5R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700096 SM3300T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700343 SAMN03022973 SM3300T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700097 SM3300T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700343 SAMN03022973 SM3300T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700098 SM3302T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700344 SAMN03022974 SM3302T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700099 SM3302T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700344 SAMN03022974 SM3302T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700100 SM3302T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700345 SAMN03022975 SM3302T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700101 SM3302T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700345 SAMN03022975 SM3302T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700102 SM3393T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700346 SAMN03022976 SM3393T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700103 SM3393T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700346 SAMN03022976 SM3393T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700104 SM3393T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700347 SAMN03022977 SM3393T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700105 SM3393T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700347 SAMN03022977 SM3393T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700106 SM3393T3L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700348 SAMN03022978 SM3393T3L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700107 SM3393T3R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700348 SAMN03022978 SM3393T3R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700108 SM3393T4L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700349 SAMN03022979 SM3393T4L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700109 SM3393T4R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700349 SAMN03022979 SM3393T4R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700110 SM3577T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700350 SAMN03022980 SM3577T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700111 SM3577T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700350 SAMN03022980 SM3577T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700112 SM3637T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700351 SAMN03022981 SM3637T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700113 SM3637T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700351 SAMN03022981 SM3637T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700114 SM3643T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700352 SAMN03022982 SM3643T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700115 SM3643T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700352 SAMN03022982 SM3643T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700116 SM3643T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700353 SAMN03022983 SM3643T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700117 SM3643T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700353 SAMN03022983 SM3643T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700118 SM3644T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700354 SAMN03022984 SM3644T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700119 SM3644T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700354 SAMN03022984 SM3644T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700120 SM3662T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700355 SAMN03022985 SM3662T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700121 SM3662T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700355 SAMN03022985 SM3662T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700122 SM3665T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700356 SAMN03022986 SM3665T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700123 SM3665T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700356 SAMN03022986 SM3665T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700124 SM3679T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700357 SAMN03022987 SM3679T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700125 SM3679T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700357 SAMN03022987 SM3679T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700126 SM3679T4L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700358 SAMN03022988 SM3679T4L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700127 SM3679T4R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700358 SAMN03022988 SM3679T4R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700128 SM3707T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700359 SAMN03022989 SM3707T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700129 SM3707T3L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700360 SAMN03022990 SM3707T3L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700130 SM3707T3R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700360 SAMN03022990 SM3707T3R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700131 SM3740T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700361 SAMN03022991 SM3740T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700132 SM3750T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700362 SAMN03022992 SM3750T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700133 SM3906T2L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700363 SAMN03022993 SM3906T2L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700134 SM3906T2R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700363 SAMN03022993 SM3906T2R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700135 SM3906T3L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700364 SAMN03022994 SM3906T3L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700136 SM4055T1L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700365 SAMN03022995 SM4055T1L Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700137 SM4055T1R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700365 SAMN03022995 SM4055T1R Tn-Seq GENOMIC PCR 101 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700138 SM100L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700366 SAMN03022996 SM100L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700139 SM100R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700366 SAMN03022996 SM100R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700140 SM101L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700367 SAMN03022997 SM101L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700141 SM101R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700367 SAMN03022997 SM101R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700142 SM102L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700368 SAMN03022998 SM102L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700143 SM102R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700368 SAMN03022998 SM102R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700144 SM103L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700369 SAMN03022999 SM103L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700145 SM103R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700369 SAMN03022999 SM103R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700146 SM104L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700370 SAMN03023000 SM104L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700147 SM104R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700370 SAMN03023000 SM104R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700148 SM105L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700372 SAMN03023001 SM105L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700149 SM105R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700372 SAMN03023001 SM105R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700150 SM106L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700373 SAMN03023002 SM106L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700151 SM106R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700373 SAMN03023002 SM106R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700152 SM107L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700374 SAMN03023003 SM107L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700153 SM107R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700374 SAMN03023003 SM107R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700154 SM108L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700375 SAMN03023004 SM108L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700155 SM108R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700375 SAMN03023004 SM108R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700156 SM109L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700376 SAMN03023005 SM109L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700157 SM109R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700376 SAMN03023005 SM109R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700158 SM110L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700377 SAMN03023006 SM110L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700159 SM110R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700377 SAMN03023006 SM110R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700160 SM111L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700378 SAMN03023007 SM111L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700161 SM111R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700378 SAMN03023007 SM111R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700162 SM112L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700379 SAMN03023008 SM112L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700163 SM112R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700379 SAMN03023008 SM112R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700164 SM113L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700380 SAMN03023009 SM113L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700165 SM113R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700380 SAMN03023009 SM113R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700166 SM114L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700381 SAMN03023010 SM114L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700167 SM114R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700381 SAMN03023010 SM114R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700168 SM115L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700382 SAMN03023011 SM115L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700169 SM115R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700382 SAMN03023011 SM115R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700170 SM116L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700383 SAMN03023012 SM116L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700171 SM116R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700383 SAMN03023012 SM116R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700172 SM117L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700384 SAMN03023013 SM117L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700173 SM117R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700384 SAMN03023013 SM117R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700174 SM118L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700385 SAMN03023014 SM118L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700175 SM118R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700385 SAMN03023014 SM118R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700176 SM119L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700386 SAMN03023015 SM119L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700177 SM119R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700386 SAMN03023015 SM119R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700178 SM120L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700387 SAMN03023016 SM120L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700179 SM120R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700387 SAMN03023016 SM120R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700180 SM121L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700388 SAMN03023017 SM121L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700181 SM121R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700388 SAMN03023017 SM121R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700182 SM122L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700389 SAMN03023018 SM122L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700183 SM122R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700389 SAMN03023018 SM122R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700184 SM123L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700391 SAMN03023019 SM123L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700185 SM123R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700391 SAMN03023019 SM123R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700186 SM82L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700392 SAMN03023020 SM82L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700187 SM82R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700392 SAMN03023020 SM82R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700188 SM83L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700393 SAMN03023021 SM83L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700189 SM83R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700393 SAMN03023021 SM83R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700190 SM84L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700394 SAMN03023022 SM84L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700191 SM84R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700394 SAMN03023022 SM84R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700192 SM85L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700395 SAMN03023023 SM85L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700193 SM85R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700395 SAMN03023023 SM85R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700194 SM86L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700396 SAMN03023024 SM86L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700195 SM86R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700396 SAMN03023024 SM86R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700196 SM87L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700397 SAMN03023025 SM87L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700197 SM87R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700397 SAMN03023025 SM87R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700198 SM88L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700398 SAMN03023026 SM88L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700199 SM88R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700398 SAMN03023026 SM88R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700200 SM89L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700399 SAMN03023027 SM89L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700201 SM89R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700399 SAMN03023027 SM89R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700202 SM90L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700400 SAMN03023028 SM90L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700203 SM90R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700400 SAMN03023028 SM90R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700204 SM91L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700401 SAMN03023029 SM91L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700205 SM91R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700401 SAMN03023029 SM91R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700206 SM92L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700402 SAMN03023030 SM92L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700207 SM92R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700402 SAMN03023030 SM92R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700208 SM93L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700403 SAMN03023031 SM93L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700209 SM93R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700403 SAMN03023031 SM93R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700210 SM94L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700404 SAMN03023032 SM94L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700211 SM94R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700404 SAMN03023032 SM94R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700212 SM95L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700405 SAMN03023033 SM95L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700213 SM95R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700405 SAMN03023033 SM95R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700214 SM96L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700406 SAMN03023034 SM96L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700215 SM96R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700406 SAMN03023034 SM96R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700216 SM97L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700407 SAMN03023035 SM97L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700217 SM97R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700407 SAMN03023035 SM97R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700218 SM98L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700408 SAMN03023036 SM98L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700219 SM98R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700408 SAMN03023036 SM98R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700220 SM99R SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700409 SAMN03023037 SM99R Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500 SRX700221 SM99L SRP047071 PRJNA260417 "Genomic DNA (gDNA) of the tumors was isolated using Puregene Tissue Kit (Qiagen). 1ug of gDNA was digested with Alu I, HaeIII or NlaIII (New England Biolabs). AluI and HaeIII digested gDNA was ligated to an IRDRL linker. HaeIII and NlaIII digested gDNA was ligated to the IRDRR linker. Subsequently, ligations were digested with BamHI to destroy concatamer-generated products. Genomic-transposon junction fragments were amplified using a nested PCR reaction and 50 ng of each reaction was pooled for a total of 60-68 barcoded PCR reactions per pooled library. Pooled libraries were sequenced on an Illumina HiSeq2500 instrument at University of Chicago Functional Genomics Facility using standard methods. Sequencing data were demultiplexed before analysis." SRS700409 SAMN03023037 SM99L Tn-Seq GENOMIC PCR 103 0 Application Read Forward 1 Illumina HiSeq 2500