SRX24174295 LJ_5069 SRP072679 PRJNA316969 DNA was extracted from liquid culture using the Qiagen DNeasy Blood and Tissue Microbial Kit following the manufacturers protocol for Gram-positive bacteria. DNA was sent to SeqCoast Genomics LLC (Portsmouth, NH USA) for library preparation and sequencing. Samples were prepared for whole genome sequencing using an Illumina DNA Prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300-cycle flow cell kit, producing 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v3.10.12. SRS20950248 SAMN40858149 LJ_5069 WGS GENOMIC RANDOM NextSeq 2000 SRX24174296 LJ_1855 SRP072679 PRJNA316969 DNA was extracted from liquid culture using the Qiagen DNeasy Blood and Tissue Microbial Kit following the manufacturers protocol for Gram-positive bacteria. DNA was sent to SeqCoast Genomics LLC (Portsmouth, NH USA) for library preparation and sequencing. Samples were prepared for whole genome sequencing using an Illumina DNA Prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300-cycle flow cell kit, producing 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v3.10.12. SRS20950249 SAMN40858150 LJ_1855 WGS GENOMIC RANDOM NextSeq 2000 SRX24174297 LG_1673 SRP072679 PRJNA316969 DNA was extracted from liquid culture using the Qiagen DNeasy Blood and Tissue Microbial Kit following the manufacturers protocol for Gram-positive bacteria. DNA was sent to SeqCoast Genomics LLC (Portsmouth, NH USA) for library preparation and sequencing. Samples were prepared for whole genome sequencing using an Illumina DNA Prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300-cycle flow cell kit, producing 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v3.10.12. SRS20950250 SAMN40858151 LG_1673 WGS GENOMIC RANDOM NextSeq 2000