SRX21032536 GSM7622011_r1 SRP449646 PRJNA994978 SRS18304966 GSM7622011 GSM7622011 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21032537 GSM7622012_r1 SRP449646 PRJNA994978 SRS18304967 GSM7622012 GSM7622012 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21032538 GSM7622013_r1 SRP449646 PRJNA994978 SRS18304968 GSM7622013 GSM7622013 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21032539 GSM7622014_r1 SRP449646 PRJNA994978 SRS18304969 GSM7622014 GSM7622014 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21032540 GSM7622015_r1 SRP449646 PRJNA994978 SRS18304970 GSM7622015 GSM7622015 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21032541 GSM7622016_r1 SRP449646 PRJNA994978 SRS18304971 GSM7622016 GSM7622016 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21032542 GSM7622017_r1 SRP449646 PRJNA994978 SRS18304972 GSM7622017 GSM7622017 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX21032543 GSM7622018_r1 SRP449646 PRJNA994978 SRS18304973 GSM7622018 GSM7622018 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX21032544 GSM7622019_r1 SRP449646 PRJNA994978 SRS18304974 GSM7622019 GSM7622019 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX21032545 GSM7622020_r1 SRP449646 PRJNA994978 SRS18304975 GSM7622020 GSM7622020 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX21032546 GSM7622021_r1 SRP449646 PRJNA994978 SRS18304976 GSM7622021 GSM7622021 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX21032547 GSM7622022_r1 SRP449646 PRJNA994978 SRS18304977 GSM7622022 GSM7622022 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX21032548 GSM7622023_r1 SRP449646 PRJNA994978 SRS18304978 GSM7622023 GSM7622023 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX21032549 GSM7622024_r1 SRP449646 PRJNA994978 SRS18304979 GSM7622024 GSM7622024 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX21755930 GSM7775314_r1 SRP449646 PRJNA994978 SRS18862704 GSM7775314 GSM7775314 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755931 GSM7775315_r1 SRP449646 PRJNA994978 SRS18862701 GSM7775315 GSM7775315 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755932 GSM7775316_r1 SRP449646 PRJNA994978 SRS18862705 GSM7775316 GSM7775316 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755933 GSM7775317_r1 SRP449646 PRJNA994978 SRS18862707 GSM7775317 GSM7775317 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755934 GSM7775318_r1 SRP449646 PRJNA994978 SRS18862706 GSM7775318 GSM7775318 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755935 GSM7775319_r1 SRP449646 PRJNA994978 SRS18862708 GSM7775319 GSM7775319 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755936 GSM7775320_r1 SRP449646 PRJNA994978 SRS18862710 GSM7775320 GSM7775320 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755937 GSM7775321_r1 SRP449646 PRJNA994978 SRS18862709 GSM7775321 GSM7775321 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755938 GSM7775322_r1 SRP449646 PRJNA994978 SRS18862711 GSM7775322 GSM7775322 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755939 GSM7775323_r1 SRP449646 PRJNA994978 SRS18862713 GSM7775323 GSM7775323 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755940 GSM7775324_r1 SRP449646 PRJNA994978 SRS18862712 GSM7775324 GSM7775324 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755941 GSM7775325_r1 SRP449646 PRJNA994978 SRS18862714 GSM7775325 GSM7775325 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755942 GSM7775326_r1 SRP449646 PRJNA994978 SRS18862717 GSM7775326 GSM7775326 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755943 GSM7775327_r1 SRP449646 PRJNA994978 SRS18862715 GSM7775327 GSM7775327 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755944 GSM7775328_r1 SRP449646 PRJNA994978 SRS18862716 GSM7775328 GSM7775328 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21755945 GSM7775329_r1 SRP449646 PRJNA994978 SRS18862718 GSM7775329 GSM7775329 RNA-Seq TRANSCRIPTOMIC cDNA Native nuclear lysates were fractioned by sucrose gradient centrifugation (6%-40% w/v). Add an equal amount of RNA spike-in (4 ERCC transcripts) to each fraction for normalization to cell number. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21804379 GSM7785436_r1 SRP449646 PRJNA994978 SRS18905377 GSM7785436 GSM7785436 Hi-C GENOMIC other Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was subjected to MboI digestion, biotin end repairing and ligation, then reverse-crosslinking and DNA purification. The purified DNA was then sonicated and subjected to biotin pull down by C1 Streptavidin beads. Library construction procedures including end repairing, dATP tailing and adaptor ligation were proceeded on beads. Finally, the DNA was eluted and subjected to PCR and size selection for sequencing. HiC libraries were constructed following TruSeq DNA preparation procedures including end repairing, dATP tailing, adaptor ligation and PCR amplification. HiSeq X Ten SRX21804380 GSM7785437_r1 SRP449646 PRJNA994978 SRS18905380 GSM7785437 GSM7785437 Hi-C GENOMIC other Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was subjected to MboI digestion, biotin end repairing and ligation, then reverse-crosslinking and DNA purification. The purified DNA was then sonicated and subjected to biotin pull down by C1 Streptavidin beads. Library construction procedures including end repairing, dATP tailing and adaptor ligation were proceeded on beads. Finally, the DNA was eluted and subjected to PCR and size selection for sequencing. HiC libraries were constructed following TruSeq DNA preparation procedures including end repairing, dATP tailing, adaptor ligation and PCR amplification. HiSeq X Ten SRX21804381 GSM7785438_r1 SRP449646 PRJNA994978 SRS18905382 GSM7785438 GSM7785438 Hi-C GENOMIC other Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was subjected to MboI digestion, biotin end repairing and ligation, then reverse-crosslinking and DNA purification. The purified DNA was then sonicated and subjected to biotin pull down by C1 Streptavidin beads. Library construction procedures including end repairing, dATP tailing and adaptor ligation were proceeded on beads. Finally, the DNA was eluted and subjected to PCR and size selection for sequencing. HiC libraries were constructed following TruSeq DNA preparation procedures including end repairing, dATP tailing, adaptor ligation and PCR amplification. HiSeq X Ten SRX21804382 GSM7785439_r1 SRP449646 PRJNA994978 SRS18905378 GSM7785439 GSM7785439 Hi-C GENOMIC other Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was subjected to MboI digestion, biotin end repairing and ligation, then reverse-crosslinking and DNA purification. The purified DNA was then sonicated and subjected to biotin pull down by C1 Streptavidin beads. Library construction procedures including end repairing, dATP tailing and adaptor ligation were proceeded on beads. Finally, the DNA was eluted and subjected to PCR and size selection for sequencing. HiC libraries were constructed following TruSeq DNA preparation procedures including end repairing, dATP tailing, adaptor ligation and PCR amplification. HiSeq X Ten SRX21804383 GSM7785440_r1 SRP449646 PRJNA994978 SRS18905379 GSM7785440 GSM7785440 Hi-C GENOMIC other Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was subjected to MboI digestion, biotin end repairing and ligation, then reverse-crosslinking and DNA purification. The purified DNA was then sonicated and subjected to biotin pull down by C1 Streptavidin beads. Library construction procedures including end repairing, dATP tailing and adaptor ligation were proceeded on beads. Finally, the DNA was eluted and subjected to PCR and size selection for sequencing. HiC libraries were constructed following TruSeq DNA preparation procedures including end repairing, dATP tailing, adaptor ligation and PCR amplification. HiSeq X Ten SRX21804384 GSM7785441_r1 SRP449646 PRJNA994978 SRS18905381 GSM7785441 GSM7785441 Hi-C GENOMIC other Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was subjected to MboI digestion, biotin end repairing and ligation, then reverse-crosslinking and DNA purification. The purified DNA was then sonicated and subjected to biotin pull down by C1 Streptavidin beads. Library construction procedures including end repairing, dATP tailing and adaptor ligation were proceeded on beads. Finally, the DNA was eluted and subjected to PCR and size selection for sequencing. HiC libraries were constructed following TruSeq DNA preparation procedures including end repairing, dATP tailing, adaptor ligation and PCR amplification. HiSeq X Ten SRX21932221 GSM7813941_r1 SRP449646 PRJNA994978 SRS19014457 GSM7813941 GSM7813941 RNA-Seq TRANSCRIPTOMIC cDNA ESC nuclei were subjected to DNase I treatment followed by sequential washes using detergents (1 M urea and 1% NP40) and high-salt (2 M NaCl). The resulting pellet, representing the 'insoluble' RNP mesh, was used for RNA-seq. Add the RNA spike-in (drosophila S2 cells) directly to samples according to number of cells. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to poly(A) purification by VAHTS mRNA Capture Beads (Vazyme) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21932222 GSM7813940_r1 SRP449646 PRJNA994978 SRS19014458 GSM7813940 GSM7813940 RNA-Seq TRANSCRIPTOMIC cDNA ESC nuclei were subjected to DNase I treatment followed by sequential washes using detergents (1 M urea and 1% NP40) and high-salt (2 M NaCl). The resulting pellet, representing the 'insoluble' RNP mesh, was used for RNA-seq. Add the RNA spike-in (drosophila S2 cells) directly to samples according to number of cells. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to poly(A) purification by VAHTS mRNA Capture Beads (Vazyme) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21932223 GSM7813939_r1 SRP449646 PRJNA994978 SRS19014459 GSM7813939 GSM7813939 RNA-Seq TRANSCRIPTOMIC cDNA ESC nuclei were subjected to DNase I treatment followed by sequential washes using detergents (1 M urea and 1% NP40) and high-salt (2 M NaCl). The resulting pellet, representing the 'insoluble' RNP mesh, was used for RNA-seq. Add the RNA spike-in (drosophila S2 cells) directly to samples according to number of cells. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to poly(A) purification by VAHTS mRNA Capture Beads (Vazyme) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21932224 GSM7813938_r1 SRP449646 PRJNA994978 SRS19014460 GSM7813938 GSM7813938 RNA-Seq TRANSCRIPTOMIC cDNA ESC nuclei were subjected to DNase I treatment followed by sequential washes using detergents (1 M urea and 1% NP40) and high-salt (2 M NaCl). The resulting pellet, representing the 'insoluble' RNP mesh, was used for RNA-seq. Add the RNA spike-in (drosophila S2 cells) directly to samples according to number of cells. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to poly(A) purification by VAHTS mRNA Capture Beads (Vazyme) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21932225 GSM7813937_r1 SRP449646 PRJNA994978 SRS19014461 GSM7813937 GSM7813937 RNA-Seq TRANSCRIPTOMIC cDNA ESC nuclei were subjected to DNase I treatment followed by sequential washes using detergents (1 M urea and 1% NP40) and high-salt (2 M NaCl). The resulting pellet, representing the 'insoluble' RNP mesh, was used for RNA-seq. Add the RNA spike-in (drosophila S2 cells) directly to samples according to number of cells. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to poly(A) purification by VAHTS mRNA Capture Beads (Vazyme) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX21932226 GSM7813936_r1 SRP449646 PRJNA994978 SRS19014462 GSM7813936 GSM7813936 RNA-Seq TRANSCRIPTOMIC cDNA ESC nuclei were subjected to DNase I treatment followed by sequential washes using detergents (1 M urea and 1% NP40) and high-salt (2 M NaCl). The resulting pellet, representing the 'insoluble' RNP mesh, was used for RNA-seq. Add the RNA spike-in (drosophila S2 cells) directly to samples according to number of cells. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to poly(A) purification by VAHTS mRNA Capture Beads (Vazyme) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX22092654 GSM7841780_r1 SRP449646 PRJNA994978 SRS19158615 GSM7841780 GSM7841780 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX22092655 GSM7841781_r1 SRP449646 PRJNA994978 SRS19158616 GSM7841781 GSM7841781 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX22092656 GSM7841798_r1 SRP449646 PRJNA994978 SRS19158617 GSM7841798 GSM7841798 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX22092657 GSM7841799_r1 SRP449646 PRJNA994978 SRS19158618 GSM7841799 GSM7841799 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX22092658 GSM7841800_r1 SRP449646 PRJNA994978 SRS19158619 GSM7841800 GSM7841800 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX22092659 GSM7841801_r1 SRP449646 PRJNA994978 SRS19158620 GSM7841801 GSM7841801 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX22092660 GSM7841782_r1 SRP449646 PRJNA994978 SRS19158621 GSM7841782 GSM7841782 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX22092661 GSM7841783_r1 SRP449646 PRJNA994978 SRS19158622 GSM7841783 GSM7841783 ChIP-Seq GENOMIC ChIP Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols. HiSeq X Ten SRX23535148 GSM8063790_r1 SRP449646 PRJNA994978 SRS20383945 GSM8063790 GSM8063790 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX23535149 GSM8063791_r1 SRP449646 PRJNA994978 SRS20383946 GSM8063791 GSM8063791 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX23535150 GSM8063792_r1 SRP449646 PRJNA994978 SRS20383947 GSM8063792 GSM8063792 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX23535151 GSM8063793_r1 SRP449646 PRJNA994978 SRS20383948 GSM8063793 GSM8063793 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX23535152 GSM8063794_r1 SRP449646 PRJNA994978 SRS20383949 GSM8063794 GSM8063794 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten SRX23535153 GSM8063795_r1 SRP449646 PRJNA994978 SRS20383950 GSM8063795 GSM8063795 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols. HiSeq X Ten