SRS702484 SAMN03009871 7130 Manduca sexta Manduca sexta midgut tissue / developmental stage RNA-Seq bioproject 81039 PRJNA81039 strain not applicable age not applicable biomaterial_provider G. Blissard / Y-R Chen sex not collected tissue Midgut collected_by Yun-Ru Chen dev_stage 2nd instar larvae tissue_collection_information Larvae were dissected longitudinally and the entire midgut was removed with scissors and forceps. The food debris in the midgut was washed away with PBS twice, then tissue was collected in Trizol and processed for RNA isolation. Samples may contain contaminating muscle, trachea and hemocytes associated with the midgut tissue. BioSampleModel Model organism or animal