SRX709648 M. capitata microbial communities SRP047463 To compare the bacterial community structure of healthy red and orange fragments of M. capitata, total DNA from coral crushate was extracted using the MoBio PowerSoil DNA Extraction kit (MoBio Carlsbad, CA USA) as per the manufacturer’s instructions with one exception; the lysis step was modified by increasing the heat lysis incubation period to 15 min with 1 min of vortexing every five minutes. 300 bp DNA fragments containing the V3 hyper-variable region of the 16S rRNA gene were amplified using a bar-coded primer set constructed for pyrosequencing (Table S1). PCR products were visualized on 2% agarose TAE gels and purified using the Qiaquick Gel Extraction Kit (QIAGEN, Inc). Purified PCR products were submitted to the Advanced Studies of Genomics, Proteomics, and Bioinformatics sequencing facility of the University of Hawaii at Manoa (http://asgpb.mhpcc.hawaii.edu/) for high-throughput sequencing on a Roche 454 Next Generation GSFLX system SRS708676 HR1 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium MacQIIME SRX709656 M. capitata microbial communities HR2 SRP047463 To compare the bacterial community structure of healthy red and orange fragments of M. capitata, total DNA from coral crushate was extracted using the MoBio PowerSoil DNA Extraction kit (MoBio Carlsbad, CA USA) as per the manufacturer’s instructions with one exception; the lysis step was modified by increasing the heat lysis incubation period to 15 min with 1 min of vortexing every five minutes. 300 bp DNA fragments containing the V3 hyper-variable region of the 16S rRNA gene were amplified using a bar-coded primer set constructed for pyrosequencing (Table S1). PCR products were visualized on 2% agarose TAE gels and purified using the Qiaquick Gel Extraction Kit (QIAGEN, Inc). Purified PCR products were submitted to the Advanced Studies of Genomics, Proteomics, and Bioinformatics sequencing facility of the University of Hawaii at Manoa (http://asgpb.mhpcc.hawaii.edu/) for high-throughput sequencing on a Roche 454 Next Generation GSFLX system. SRS708684 SAMN03078475 HR2 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium MacQIIME SRX733587 M. capitata microbial communities HR3 SRP047463 To compare the bacterial community structure of healthy red and orange fragments of M. capitata, total DNA from coral crushate was extracted using the MoBio PowerSoil DNA Extraction kit (MoBio Carlsbad, CA USA) as per the manufacturer’s instructions with one exception; the lysis step was modified by increasing the heat lysis incubation period to 15 min with 1 min of vortexing every five minutes. 300 bp DNA fragments containing the V3 hyper-variable region of the 16S rRNA gene were amplified using a bar-coded primer set constructed for pyrosequencing (Table S1). PCR products were visualized on 2% agarose TAE gels and purified using the Qiaquick Gel Extraction Kit (QIAGEN, Inc). Purified PCR products were submitted to the Advanced Studies of Genomics, Proteomics, and Bioinformatics sequencing facility of the University of Hawaii at Manoa (http://asgpb.mhpcc.hawaii.edu/) for high-throughput sequencing on a Roche 454 Next Generation GSFLX system SRS722125 HR3 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium MacQIIME SRX733594 M. capitata microbial communities HR4 SRP047463 To compare the bacterial community structure of healthy red and orange fragments of M. capitata, total DNA from coral crushate was extracted using the MoBio PowerSoil DNA Extraction kit (MoBio Carlsbad, CA USA) as per the manufacturer’s instructions with one exception; the lysis step was modified by increasing the heat lysis incubation period to 15 min with 1 min of vortexing every five minutes. 300 bp DNA fragments containing the V3 hyper-variable region of the 16S rRNA gene were amplified using a bar-coded primer set constructed for pyrosequencing (Table S1). PCR products were visualized on 2% agarose TAE gels and purified using the Qiaquick Gel Extraction Kit (QIAGEN, Inc). Purified PCR products were submitted to the Advanced Studies of Genomics, Proteomics, and Bioinformatics sequencing facility of the University of Hawaii at Manoa (http://asgpb.mhpcc.hawaii.edu/) for high-throughput sequencing on a Roche 454 Next Generation GSFLX system SRS722129 SAMN03078477 HR4 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium MacQIIME SRX733596 M. capitata microbial communities HR5 SRP047463 To compare the bacterial community structure of healthy red and orange fragments of M. capitata, total DNA from coral crushate was extracted using the MoBio PowerSoil DNA Extraction kit (MoBio Carlsbad, CA USA) as per the manufacturer’s instructions with one exception; the lysis step was modified by increasing the heat lysis incubation period to 15 min with 1 min of vortexing every five minutes. 300 bp DNA fragments containing the V3 hyper-variable region of the 16S rRNA gene were amplified using a bar-coded primer set constructed for pyrosequencing (Table S1). PCR products were visualized on 2% agarose TAE gels and purified using the Qiaquick Gel Extraction Kit (QIAGEN, Inc). Purified PCR products were submitted to the Advanced Studies of Genomics, Proteomics, and Bioinformatics sequencing facility of the University of Hawaii at Manoa (http://asgpb.mhpcc.hawaii.edu/) for high-throughput sequencing on a Roche 454 Next Generation GSFLX system SRS722130 HR5 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium MacQIIME SRX733597 M. capitata microbial communities HO1 SRP047463 To compare the bacterial community structure of healthy red and orange fragments of M. capitata, total DNA from coral crushate was extracted using the MoBio PowerSoil DNA Extraction kit (MoBio Carlsbad, CA USA) as per the manufacturer’s instructions with one exception; the lysis step was modified by increasing the heat lysis incubation period to 15 min with 1 min of vortexing every five minutes. 300 bp DNA fragments containing the V3 hyper-variable region of the 16S rRNA gene were amplified using a bar-coded primer set constructed for pyrosequencing (Table S1). PCR products were visualized on 2% agarose TAE gels and purified using the Qiaquick Gel Extraction Kit (QIAGEN, Inc). Purified PCR products were submitted to the Advanced Studies of Genomics, Proteomics, and Bioinformatics sequencing facility of the University of Hawaii at Manoa (http://asgpb.mhpcc.hawaii.edu/) for high-throughput sequencing on a Roche 454 Next Generation GSFLX system SRS722131 SAMN03078479 HO1 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium MacQIIME SRX733599 M. capitata microbial communities HO2 SRP047463 To compare the bacterial community structure of healthy red and orange fragments of M. capitata, total DNA from coral crushate was extracted using the MoBio PowerSoil DNA Extraction kit (MoBio Carlsbad, CA USA) as per the manufacturer’s instructions with one exception; the lysis step was modified by increasing the heat lysis incubation period to 15 min with 1 min of vortexing every five minutes. 300 bp DNA fragments containing the V3 hyper-variable region of the 16S rRNA gene were amplified using a bar-coded primer set constructed for pyrosequencing (Table S1). PCR products were visualized on 2% agarose TAE gels and purified using the Qiaquick Gel Extraction Kit (QIAGEN, Inc). Purified PCR products were submitted to the Advanced Studies of Genomics, Proteomics, and Bioinformatics sequencing facility of the University of Hawaii at Manoa (http://asgpb.mhpcc.hawaii.edu/) for high-throughput sequencing on a Roche 454 Next Generation GSFLX system SRS722132 SAMN03078480 HO2 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium MacQIIME SRX733600 M. capitata microbial communities HO3 SRP047463 To compare the bacterial community structure of healthy red and orange fragments of M. capitata, total DNA from coral crushate was extracted using the MoBio PowerSoil DNA Extraction kit (MoBio Carlsbad, CA USA) as per the manufacturer’s instructions with one exception; the lysis step was modified by increasing the heat lysis incubation period to 15 min with 1 min of vortexing every five minutes. 300 bp DNA fragments containing the V3 hyper-variable region of the 16S rRNA gene were amplified using a bar-coded primer set constructed for pyrosequencing (Table S1). PCR products were visualized on 2% agarose TAE gels and purified using the Qiaquick Gel Extraction Kit (QIAGEN, Inc). Purified PCR products were submitted to the Advanced Studies of Genomics, Proteomics, and Bioinformatics sequencing facility of the University of Hawaii at Manoa (http://asgpb.mhpcc.hawaii.edu/) for high-throughput sequencing on a Roche 454 Next Generation GSFLX system SRS722133 SAMN03078481 HO3 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium MacQIIME SRX733602 M. capitata microbial communities HO4 SRP047463 To compare the bacterial community structure of healthy red and orange fragments of M. capitata, total DNA from coral crushate was extracted using the MoBio PowerSoil DNA Extraction kit (MoBio Carlsbad, CA USA) as per the manufacturer’s instructions with one exception; the lysis step was modified by increasing the heat lysis incubation period to 15 min with 1 min of vortexing every five minutes. 300 bp DNA fragments containing the V3 hyper-variable region of the 16S rRNA gene were amplified using a bar-coded primer set constructed for pyrosequencing (Table S1). PCR products were visualized on 2% agarose TAE gels and purified using the Qiaquick Gel Extraction Kit (QIAGEN, Inc). Purified PCR products were submitted to the Advanced Studies of Genomics, Proteomics, and Bioinformatics sequencing facility of the University of Hawaii at Manoa (http://asgpb.mhpcc.hawaii.edu/) for high-throughput sequencing on a Roche 454 Next Generation GSFLX system SRS722134 SAMN03078482 HO4 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium MacQIIME SRX733603 M. capitata microbial communities HO5 SRP047463 To compare the bacterial community structure of healthy red and orange fragments of M. capitata, total DNA from coral crushate was extracted using the MoBio PowerSoil DNA Extraction kit (MoBio Carlsbad, CA USA) as per the manufacturer’s instructions with one exception; the lysis step was modified by increasing the heat lysis incubation period to 15 min with 1 min of vortexing every five minutes. 300 bp DNA fragments containing the V3 hyper-variable region of the 16S rRNA gene were amplified using a bar-coded primer set constructed for pyrosequencing (Table S1). PCR products were visualized on 2% agarose TAE gels and purified using the Qiaquick Gel Extraction Kit (QIAGEN, Inc). Purified PCR products were submitted to the Advanced Studies of Genomics, Proteomics, and Bioinformatics sequencing facility of the University of Hawaii at Manoa (http://asgpb.mhpcc.hawaii.edu/) for high-throughput sequencing on a Roche 454 Next Generation GSFLX system SRS722135 SAMN03078483 HO5 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium MacQIIME