SRX751567 GSM1537957 SRP049554 PRJNA266433 SRS737189 SAMN03164248 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min (2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301537957 GSM1537957 GEO Accession GSM1537957 SRX751568 GSM1537958 SRP049554 PRJNA266433 SRS737190 SAMN03164246 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min (2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301537958 GSM1537958 GEO Accession GSM1537958 SRX751569 GSM1537959 SRP049554 PRJNA266433 SRS737191 SAMN03164245 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min (2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301537959 GSM1537959 GEO Accession GSM1537959 SRX751570 GSM1537960 SRP049554 PRJNA266433 SRS737192 SAMN03164243 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min (2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301537960 GSM1537960 GEO Accession GSM1537960 SRX751571 GSM1537961 SRP049554 PRJNA266433 SRS737193 SAMN03164244 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min (2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301537961 GSM1537961 GEO Accession GSM1537961 SRX751572 GSM1537962 SRP049554 PRJNA266433 SRS737194 SAMN03164247 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min (2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301537962 GSM1537962 GEO Accession GSM1537962 SRX1050602 GSM1704739 SRP049554 PRJNA266433 SRS954806 SAMN03763721 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min (2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl CDK12 polyclonal antibody were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104) and eluted in TE buffer. DNA was end-repaired, adenylated, and ligated to TruSeq sequencing adaptors. DNA was purified by AMPure beads according to the DNA amount after each step. Library DNA was amplified by Phusion HF (NEB, Cat. M0530L). 2% agarose gel was used to select 200-500bp DNA fragments after PCR amplification. Illumina HiSeq 1500 gds 301704739 GSM1704739 GEO Accession GSM1704739 SRX1050603 GSM1704740 SRP049554 PRJNA266433 SRS954805 SAMN03763722 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min (2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl CDK12 polyclonal antibody were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104) and eluted in TE buffer. DNA was end-repaired, adenylated, and ligated to TruSeq sequencing adaptors. DNA was purified by AMPure beads according to the DNA amount after each step. Library DNA was amplified by Phusion HF (NEB, Cat. M0530L). 2% agarose gel was used to select 200-500bp DNA fragments after PCR amplification. Illumina HiSeq 1500 gds 301704740 GSM1704740 GEO Accession GSM1704740 SRX1167417 GSM1863193 SRP049554 PRJNA266433 SRS1047349 SAMN04017556 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min(2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104 ) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301863193 GSM1863193 GEO Accession GSM1863193 SRX1167418 GSM1863194 SRP049554 PRJNA266433 SRS1047348 SAMN04017557 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min(2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104 ) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301863194 GSM1863194 GEO Accession GSM1863194 SRX1167419 GSM1863195 SRP049554 PRJNA266433 SRS1047347 SAMN04017558 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min(2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104 ) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301863195 GSM1863195 GEO Accession GSM1863195 SRX1167420 GSM1863196 SRP049554 PRJNA266433 SRS1047346 SAMN04017559 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min(2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104 ) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301863196 GSM1863196 GEO Accession GSM1863196 SRX1167421 GSM1863197 SRP049554 PRJNA266433 SRS1047345 SAMN04017560 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min(2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104 ) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301863197 GSM1863197 GEO Accession GSM1863197 SRX1167422 GSM1863198 SRP049554 PRJNA266433 SRS1047344 SAMN04017561 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min(2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl HP1a polyclonal antibody or 3μl H3K9me2 antibody (Ab1220) were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104 ) and eluted in TE buffer. Libraries were constructed according to Hiseq 2500 rapid SR50 one flowcell sequencing instructions Illumina HiSeq 2500 gds 301863198 GSM1863198 GEO Accession GSM1863198 SRX1170639 GSM1863991 SRP049554 PRJNA266433 SRS1048956 SAMN04018168 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min (2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl CDK12 polyclonal antibody were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104) and eluted in TE buffer. DNA was end-repaired, adenylated, and ligated to TruSeq sequencing adaptors. DNA was purified by AMPure beads according to the DNA amount after each step. Library DNA was amplified by Phusion HF (NEB, Cat. M0530L). 2% agarose gel was used to select 200-500bp DNA fragments after PCR amplification. Illumina HiSeq 1500 gds 301863991 GSM1863991 GEO Accession GSM1863991 SRX1170640 GSM1863992 SRP049554 PRJNA266433 SRS1048955 SAMN04018169 ChIP-Seq GENOMIC ChIP Using a ceramic mortar and pestle chilled with liquid N2, frozen larvae were ground to a powder and transferred to a homogenizer in ice-cold PBS. At room temperature, the homogenized cell suspensions were cross-linked with 1% formaldehyde for 20 min, quenched with 125 mM glycine for 5 min and filtered through Miracloth. Chromatin pellets were collected and resuspended in ice-cold RIPA buffer, followed by sonicating in a Branson Digital Sonifer with 25% output for 10 min (2s on, 4 s off). For immunoprecipitation, Protein A Sepharose™ Fast Flow (GE Healthcare 17-5280-01) was pre-blocked, 4μl CDK12 polyclonal antibody were used for each immunoprecipitation. The input DNA and the IP DNA were purified with a PCR purification Kit (Qiagen28104) and eluted in TE buffer. DNA was end-repaired, adenylated, and ligated to TruSeq sequencing adaptors. DNA was purified by AMPure beads according to the DNA amount after each step. Library DNA was amplified by Phusion HF (NEB, Cat. M0530L). 2% agarose gel was used to select 200-500bp DNA fragments after PCR amplification. Illumina HiSeq 1500 gds 301863992 GSM1863992 GEO Accession GSM1863992