SRX763494 GSM1550090 SRP050036 SRS747951 GSM1550090 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550090 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550090 gds 301550090 GEO Accession GSM1550090 SRX763495 GSM1550091 SRP050036 SRS747952 GSM1550091 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550091 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550091 gds 301550091 GEO Accession GSM1550091 SRX763496 GSM1550092 SRP050036 SRS747953 GSM1550092 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550092 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550092 gds 301550092 GEO Accession GSM1550092 SRX763497 GSM1550093 SRP050036 SRS747954 GSM1550093 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550093 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550093 gds 301550093 GEO Accession GSM1550093 SRX763498 GSM1550094 SRP050036 SRS747955 GSM1550094 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550094 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550094 gds 301550094 GEO Accession GSM1550094 SRX763499 GSM1550095 SRP050036 SRS747956 GSM1550095 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550095 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550095 gds 301550095 GEO Accession GSM1550095 SRX763500 GSM1550096 SRP050036 SRS747957 GSM1550096 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550096 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550096 gds 301550096 GEO Accession GSM1550096 SRX763501 GSM1550097 SRP050036 SRS747958 GSM1550097 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550097 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550097 gds 301550097 GEO Accession GSM1550097 SRX763502 GSM1550098 SRP050036 SRS747959 GSM1550098 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550098 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550098 gds 301550098 GEO Accession GSM1550098 SRX763503 GSM1550099 SRP050036 SRS747960 GSM1550099 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550099 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550099 gds 301550099 GEO Accession GSM1550099 SRX763504 GSM1550100 SRP050036 SRS747961 GSM1550100 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550100 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550100 gds 301550100 GEO Accession GSM1550100 SRX763505 GSM1550101 SRP050036 SRS747962 GSM1550101 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550101 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550101 gds 301550101 GEO Accession GSM1550101 SRX763506 GSM1550102 SRP050036 SRS747963 GSM1550102 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550102 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550102 gds 301550102 GEO Accession GSM1550102 SRX763507 GSM1550103 SRP050036 SRS747964 GSM1550103 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550103 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550103 gds 301550103 GEO Accession GSM1550103 SRX763508 GSM1550104 SRP050036 SRS747965 GSM1550104 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550104 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550104 gds 301550104 GEO Accession GSM1550104 SRX763509 GSM1550105 SRP050036 SRS747966 GSM1550105 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550105 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550105 gds 301550105 GEO Accession GSM1550105 SRX763510 GSM1550106 SRP050036 SRS747967 GSM1550106 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550106 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550106 gds 301550106 GEO Accession GSM1550106 SRX763511 GSM1550107 SRP050036 SRS747968 GSM1550107 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550107 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550107 gds 301550107 GEO Accession GSM1550107 SRX763512 GSM1550108 SRP050036 SRS747969 GSM1550108 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550108 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550108 gds 301550108 GEO Accession GSM1550108 SRX763513 GSM1550109 SRP050036 SRS747970 GSM1550109 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550109 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550109 gds 301550109 GEO Accession GSM1550109 SRX763514 GSM1550110 SRP050036 SRS747971 GSM1550110 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550110 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550110 gds 301550110 GEO Accession GSM1550110 SRX763515 GSM1550111 SRP050036 SRS747972 GSM1550111 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550111 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550111 gds 301550111 GEO Accession GSM1550111 SRX763516 GSM1550112 SRP050036 SRS747973 GSM1550112 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550112 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550112 gds 301550112 GEO Accession GSM1550112 SRX763517 GSM1550113 SRP050036 SRS747974 GSM1550113 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550113 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550113 gds 301550113 GEO Accession GSM1550113 SRX763518 GSM1550114 SRP050036 SRS747975 GSM1550114 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol following standard protocol. 1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform. Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation. This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol. Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol Illumina HiSeq 2000 GEO Sample GSM1550114 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550114 gds 301550114 GEO Accession GSM1550114