SRX763884 GSM1550372 SRP050048 SRS748259 GSM1550372 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA from BMDMs was isolated with TRIzol reagent (Life Technologies) according to the manufacturer protocol. Ribosomal RNA was depleted using Ribo-Zero rRNA removal kit (Epicenter). RNA was fragmented using RNA fragmentation reagent (Ambion). Enzymatically 5’-pre-adenylated RNA adaptor was ligated to RNA 3’-end. cDNA was produced using primer specific to 3’-adaptor sequence. cDNA was size selected (200-300 nt) using non-denaturing gel, recovered and circularized using Circligase II (Epicenter). DNA oligo complementary to BamHI site was annealed and digested with BamHI (ThermoScientific) in order to linearize cDNA. cDNA was the PCR-amplified with primers specific for linker regions. Illumina HiSeq 2000 GEO Sample GSM1550372 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550372 gds 301550372 GEO Accession GSM1550372 SRX763885 GSM1550373 SRP050048 SRS748258 GSM1550373 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA from BMDMs was isolated with TRIzol reagent (Life Technologies) according to the manufacturer protocol. Ribosomal RNA was depleted using Ribo-Zero rRNA removal kit (Epicenter). RNA was fragmented using RNA fragmentation reagent (Ambion). Enzymatically 5’-pre-adenylated RNA adaptor was ligated to RNA 3’-end. cDNA was produced using primer specific to 3’-adaptor sequence. cDNA was size selected (200-300 nt) using non-denaturing gel, recovered and circularized using Circligase II (Epicenter). DNA oligo complementary to BamHI site was annealed and digested with BamHI (ThermoScientific) in order to linearize cDNA. cDNA was the PCR-amplified with primers specific for linker regions. Illumina HiSeq 2000 GEO Sample GSM1550373 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1550373 gds 301550373 GEO Accession GSM1550373