SRX764798 GSM1551308 SRP050089 SRS749157 GSM1551308 RNA-Seq TRANSCRIPTOMIC cDNA Tissue blocks were provided from NICHD brain bank. Total RNA was extracted from flash frozen tissue samples using Trizol reagent. Then the total RNA was treated with RNase-Free DNase (Qiagen) followed by purification with RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions. 2 µg of total RNA each sample was subject to RNA-seq library preparation with ScriptSeq™ Complete Gold Kit from Epicentre (Cat. # SCL24EP, Madison, WI) following the manufacturer’s instructions. In brief, ribosomal RNA was depleted from total RNA using Ribo-Zero magnetic beads, then the ribosomal RNA depleted RNA was purified and fragmented. Random primer tailed with Illumina adaptor was used to perform reverse transcription to get cDNA library. Adaptor sequence was added to the other end of cDNA library with a Terminal-Tagging step. cDNA library was amplified with Illumina primers provided with this kit. The product was size selected (350~500 bp) from 2% agarose E-gels (Invitrogen) and sequenced in 1 lane per sample on Illumina’s HiSeq 2000 platform. Illumina HiSeq 2000 GEO Sample GSM1551308 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1551308 gds 301551308 GEO Accession GSM1551308 SRX764799 GSM1551309 SRP050089 SRS749158 GSM1551309 RNA-Seq TRANSCRIPTOMIC cDNA Tissue blocks were provided from NICHD brain bank. Total RNA was extracted from flash frozen tissue samples using Trizol reagent. Then the total RNA was treated with RNase-Free DNase (Qiagen) followed by purification with RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions. 2 µg of total RNA each sample was subject to RNA-seq library preparation with ScriptSeq™ Complete Gold Kit from Epicentre (Cat. # SCL24EP, Madison, WI) following the manufacturer’s instructions. In brief, ribosomal RNA was depleted from total RNA using Ribo-Zero magnetic beads, then the ribosomal RNA depleted RNA was purified and fragmented. Random primer tailed with Illumina adaptor was used to perform reverse transcription to get cDNA library. Adaptor sequence was added to the other end of cDNA library with a Terminal-Tagging step. cDNA library was amplified with Illumina primers provided with this kit. The product was size selected (350~500 bp) from 2% agarose E-gels (Invitrogen) and sequenced in 1 lane per sample on Illumina’s HiSeq 2000 platform. Illumina HiSeq 2000 GEO Sample GSM1551309 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1551309 gds 301551309 GEO Accession GSM1551309 SRX764800 GSM1551310 SRP050089 SRS749159 GSM1551310 RNA-Seq TRANSCRIPTOMIC cDNA Tissue blocks were provided from NICHD brain bank. Total RNA was extracted from flash frozen tissue samples using Trizol reagent. Then the total RNA was treated with RNase-Free DNase (Qiagen) followed by purification with RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions. 2 µg of total RNA each sample was subject to RNA-seq library preparation with ScriptSeq™ Complete Gold Kit from Epicentre (Cat. # SCL24EP, Madison, WI) following the manufacturer’s instructions. In brief, ribosomal RNA was depleted from total RNA using Ribo-Zero magnetic beads, then the ribosomal RNA depleted RNA was purified and fragmented. Random primer tailed with Illumina adaptor was used to perform reverse transcription to get cDNA library. Adaptor sequence was added to the other end of cDNA library with a Terminal-Tagging step. cDNA library was amplified with Illumina primers provided with this kit. The product was size selected (350~500 bp) from 2% agarose E-gels (Invitrogen) and sequenced in 1 lane per sample on Illumina’s HiSeq 2000 platform. Illumina HiSeq 2000 GEO Sample GSM1551310 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1551310 gds 301551310 GEO Accession GSM1551310