SRX796015 SP1 SRP050522 PRJNA268141 A marketable commercial sweet potato (Ipomea batatas) root acquired in Barcelona was planted on soil to produce fresh tissue that was further propagated through cuttings at CRAG facilities. Presence of Sweet potato feathery mottle virus (SPFMV) in the original plant was confirmed by specific RT-PCR and sequencing of several genome fragments, including the complete P1 coding region where the existence of an embedded PISPO region was also confirmed. To boost accumulation of the potyvirus in the sweet potato plant, co-infection with an isolate of the crinivirus Sweet potato chlorotic stunt virus (SPCSV) was achieved by inoculation with 50 viroliferous Bemisia tabaci whiteflies, using 48 h acquisition and inoculation periods 1 . The Spanish isolate of SPCSV was kindly provided by Dr. Jesús Navas-Castillo (IHSM La Mayora, CSIC-UMA, Málaga, Spain) in an Ipomea setosa plant, also propagated through cuttings. Sweet potato virus C (SPVC) was found to be co-infecting the sweet potato plant after deep sequencing analysis. Symptomatic tissues of infected plants were collected for RNA extraction with Trizol reagent, following the provider instructions. Contaminating DNA from RNA extractions was removed with Turbo DNase (Ambion) and RNA was further purified using the RNeasy Mini kit (Quiagen).RNA libraries were constructed with the ScripSeq Complete Kit (epicentre) including barcoding elements to identify the different samples, according to provider protocols. Libraries were submitted to BGI for Illumina-Sequencing SRS779488 SAMN03203478 SP1 RNA-Seq TRANSCRIPTOMIC RANDOM 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 SRX796076 SP2 SRP050522 A marketable commercial sweet potato (Ipomea batatas) root acquired in Barcelona was planted on soil to produce fresh tissue that was further propagated through cuttings at CRAG facilities. Presence of Sweet potato feathery mottle virus (SPFMV) in the original plant was confirmed by specific RT-PCR and sequencing of several genome fragments, including the complete P1 coding region where the existence of an embedded PISPO region was also confirmed. To boost accumulation of the potyvirus in the sweet potato plant, co-infection with an isolate of the crinivirus Sweet potato chlorotic stunt virus (SPCSV) was achieved by inoculation with 50 viroliferous Bemisia tabaci whiteflies, using 48 h acquisition and inoculation periods 1 . The Spanish isolate of SPCSV was kindly provided by Dr. Jesús Navas-Castillo (IHSM La Mayora, CSIC-UMA, Málaga, Spain) in an Ipomea setosa plant, also propagated through cuttings. Sweet potato virus C (SPVC) was found to be co-infecting the sweet potato plant after deep sequencing analysis. Symptomatic tissues of infected plants were collected for RNA extraction with Trizol reagent, following the provider instructions. Contaminating DNA from RNA extractions was removed with Turbo DNase (Ambion) and RNA was further purified using the RNeasy Mini kit (Quiagen).RNA libraries were constructed with the ScripSeq Complete Kit (epicentre) including barcoding elements to identify the different samples, according to provider protocols. Libraries were submitted to BGI for Illumina-Sequencing. SRS779546 SP2 RNA-Seq TRANSCRIPTOMIC RANDOM 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 SRX796129 SP3 SRP050522 A marketable commercial sweet potato (Ipomea batatas) root acquired in Barcelona was planted on soil to produce fresh tissue that was further propagated through cuttings at CRAG facilities. Presence of Sweet potato feathery mottle virus (SPFMV) in the original plant was confirmed by specific RT-PCR and sequencing of several genome fragments, including the complete P1 coding region where the existence of an embedded PISPO region was also confirmed. To boost accumulation of the potyvirus in the sweet potato plant, co-infection with an isolate of the crinivirus Sweet potato chlorotic stunt virus (SPCSV) was achieved by inoculation with 50 viroliferous Bemisia tabaci whiteflies, using 48 h acquisition and inoculation periods 1 . The Spanish isolate of SPCSV was kindly provided by Dr. Jesús Navas-Castillo (IHSM La Mayora, CSIC-UMA, Málaga, Spain) in an Ipomea setosa plant, also propagated through cuttings. Sweet potato virus C (SPVC) was found to be co-infecting the sweet potato plant after deep sequencing analysis. Symptomatic tissues of infected plants were collected for RNA extraction with Trizol reagent, following the provider instructions. Contaminating DNA from RNA extractions was removed with Turbo DNase (Ambion) and RNA was further purified using the RNeasy Mini kit (Quiagen).RNA libraries were constructed with the ScripSeq Complete Kit (epicentre) including barcoding elements to identify the different samples, according to provider protocols. Libraries were submitted to BGI for Illumina-Sequencing SRS779594 SAMN03203482 SP3 RNA-Seq TRANSCRIPTOMIC RANDOM 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 SRX796133 SP4 SRP050522 A marketable commercial sweet potato (Ipomea batatas) root acquired in Barcelona was planted on soil to produce fresh tissue that was further propagated through cuttings at CRAG facilities. Presence of Sweet potato feathery mottle virus (SPFMV) in the original plant was confirmed by specific RT-PCR and sequencing of several genome fragments, including the complete P1 coding region where the existence of an embedded PISPO region was also confirmed. To boost accumulation of the potyvirus in the sweet potato plant, co-infection with an isolate of the crinivirus Sweet potato chlorotic stunt virus (SPCSV) was achieved by inoculation with 50 viroliferous Bemisia tabaci whiteflies, using 48 h acquisition and inoculation periods 1 . The Spanish isolate of SPCSV was kindly provided by Dr. Jesús Navas-Castillo (IHSM La Mayora, CSIC-UMA, Málaga, Spain) in an Ipomea setosa plant, also propagated through cuttings. Sweet potato virus C (SPVC) was found to be co-infecting the sweet potato plant after deep sequencing analysis. Symptomatic tissues of infected plants were collected for RNA extraction with Trizol reagent, following the provider instructions. Contaminating DNA from RNA extractions was removed with Turbo DNase (Ambion) and RNA was further purified using the RNeasy Mini kit (Quiagen).RNA libraries were constructed with the ScripSeq Complete Kit (epicentre) including barcoding elements to identify the different samples, according to provider protocols. Libraries were submitted to BGI for Illumina-Sequencing SRS779598 SAMN03203483 SP4 RNA-Seq TRANSCRIPTOMIC RANDOM 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000