SRX766223 UT6684_shortReads SRP050173 Library preparationwas performed at Genotypic Technology’s Genomics facility followingNEXTFlex DNA library protocol outlined in “NEXTFlex DNA sample preparation guide (Cat # 5140-02).~3µg of genomic DNA was sonicatedusing Bioruptorto obtain 300 to 600 bp fragment size. The size distribution was checked by running an aliquot of the sample on Agilent HS DNA Chip. The resulting fragmented DNA was cleaned up using Agencourt AMPure XP SPRI beads (Beckman Coulter). Fragmented DNA was subjected to a series of enzymatic reactions that repair frayed ends, phosphorylate the fragments, and add a single nucleotide A overhang and ligate adaptors (NEXTFlex DNA Sequencing kit). Sample cleanup was done using AMPure SPRI beads. After ligation-cleanup, ~300–600bp fragmentswas size selected on 2% low melting agarose geland cleaned using MinElute column (QIAGEN). PCR (10 cycles) amplification of adaptor ligated fragments was done and cleaned up using AMPure SPRI beads. SRS750391 SAMN03200169 Ut_Ch6684_SI_ePCR1_IL_WGS WGS GENOMIC other 602 0 Application Read Forward 1 1 Application Read Reverse 302 Illumina MiSeq