SRX767025 GSM1553164 SRP050194 SRS751160 GSM1553164 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Ion Torrent PGM GEO Sample GSM1553164 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1553164 gds 301553164 GEO Accession GSM1553164 SRX767026 GSM1553165 SRP050194 SRS751161 GSM1553165 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Ion Torrent PGM GEO Sample GSM1553165 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1553165 gds 301553165 GEO Accession GSM1553165 SRX767027 GSM1553166 SRP050194 SRS751162 GSM1553166 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Ion Torrent PGM GEO Sample GSM1553166 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1553166 gds 301553166 GEO Accession GSM1553166 SRX767028 GSM1553167 SRP050194 SRS751163 GSM1553167 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Ion Torrent PGM GEO Sample GSM1553167 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1553167 gds 301553167 GEO Accession GSM1553167 SRX767029 GSM1553168 SRP050194 SRS751164 GSM1553168 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Ion Torrent PGM GEO Sample GSM1553168 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1553168 gds 301553168 GEO Accession GSM1553168 SRX767030 GSM1553169 SRP050194 SRS751165 GSM1553169 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Ion Torrent PGM GEO Sample GSM1553169 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1553169 gds 301553169 GEO Accession GSM1553169 SRX767031 GSM1553170 SRP050194 SRS751166 GSM1553170 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Ion Torrent PGM GEO Sample GSM1553170 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1553170 gds 301553170 GEO Accession GSM1553170 SRX1077036 GSM1726485 SRP050194 SRS975921 GSM1726485 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Okazaki-fragment (OF) sequencing Ion Torrent PGM gds 301726485 GEO Accession GSM1726485 SRX1077037 GSM1726486 SRP050194 SRS975924 GSM1726486 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Okazaki-fragment (OF) sequencing Ion Torrent PGM gds 301726486 GEO Accession GSM1726486 SRX1077038 GSM1726487 SRP050194 SRS975923 GSM1726487 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Okazaki-fragment (OF) sequencing Ion Torrent PGM gds 301726487 GEO Accession GSM1726487 SRX1077039 GSM1726488 SRP050194 SRS975914 GSM1726488 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Okazaki-fragment (OF) sequencing Ion Torrent PGM gds 301726488 GEO Accession GSM1726488 SRX1077040 GSM1726489 SRP050194 SRS975915 GSM1726489 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Okazaki-fragment (OF) sequencing Ion Torrent PGM gds 301726489 GEO Accession GSM1726489 SRX1077041 GSM1726490 SRP050194 SRS975918 GSM1726490 OTHER GENOMIC other Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol Okazaki-fragment (OF) sequencing Ion Torrent PGM gds 301726490 GEO Accession GSM1726490