SRX807439 Phel_sswd_exposure SRP051104 Pycnopodia helianthoides (radius 160.5 +/- 30.9 mm) were collected from four sites in Washington State: Langley (LA) (48.038, -122.404); Dabob Bay (DB) (47.813, -122.820); Port Hadlock Marina (PH) (48.030, -122.745); Friday Harbor (FH) (48.545, -123.012). Sites had no reports of SSWD infection at time of collection. Individuals were inoculated with tissue homogenate prepared from the tube feet, dermal tissue, and coelomic fluid of three P. helianthoides with clinical signs of SSWD. The tissue was ground with a mortar and pestle, then placed in a Stomacher with 10 mL seawater, and centrifuged at 1000 x g for 5 min at 4°C. Organisms injected with the heat-killed homogenate comprised the control group. The treated group were injected with viral-sized fraction homogenate that was syringe-filtered through a 0.22 µm polyethylsulfone filter. Libraries were prepared using the Illumina TruSeq RNA Sample Preparation kit according to the manufacturer’s protocol SRS789009 SAMN03263886 RNA-Seq TRANSCRIPTOMIC PolyA 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 SRX894055 Treated_PH SRP051104 Pycnopodia helianthoides (radius 160.5 +/- 30.9 mm) were collected from four sites in Washington State: Langley (LA) (48.038, -122.404); Dabob Bay (DB) (47.813, -122.820); Port Hadlock Marina (PH) (48.030, -122.745); Friday Harbor (FH) (48.545, -123.012). Sites had no reports of SSWD infection at time of collection. Individuals were inoculated with tissue homogenate prepared from the tube feet, dermal tissue, and coelomic fluid of three P. helianthoides with clinical signs of SSWD. The tissue was ground with a mortar and pestle, then placed in a Stomacher with 10 mL seawater, and centrifuged at 1000 x g for 5 min at 4°C. Organisms injected with the heat-killed homogenate comprised the control group. The treated group were injected with viral-sized fraction homogenate that was syringe-filtered through a 0.22 µm polyethylsulfone filter. Libraries were prepared using the Illumina TruSeq RNA Sample Preparation kit according to the manufacturer’s protocol SRS860070 SAMN03263887 RNA-Seq TRANSCRIPTOMIC PolyA 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 SRX894056 Treated_L SRP051104 Pycnopodia helianthoides (radius 160.5 +/- 30.9 mm) were collected from four sites in Washington State: Langley (LA) (48.038, -122.404); Dabob Bay (DB) (47.813, -122.820); Port Hadlock Marina (PH) (48.030, -122.745); Friday Harbor (FH) (48.545, -123.012). Sites had no reports of SSWD infection at time of collection. Individuals were inoculated with tissue homogenate prepared from the tube feet, dermal tissue, and coelomic fluid of three P. helianthoides with clinical signs of SSWD. The tissue was ground with a mortar and pestle, then placed in a Stomacher with 10 mL seawater, and centrifuged at 1000 x g for 5 min at 4°C. Organisms injected with the heat-killed homogenate comprised the control group. The treated group were injected with viral-sized fraction homogenate that was syringe-filtered through a 0.22 µm polyethylsulfone filter. Libraries were prepared using the Illumina TruSeq RNA Sample Preparation kit according to the manufacturer’s protocol SRS860069 SAMN03263888 RNA-Seq TRANSCRIPTOMIC PolyA 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 SRX894057 Control_PH SRP051104 Pycnopodia helianthoides (radius 160.5 +/- 30.9 mm) were collected from four sites in Washington State: Langley (LA) (48.038, -122.404); Dabob Bay (DB) (47.813, -122.820); Port Hadlock Marina (PH) (48.030, -122.745); Friday Harbor (FH) (48.545, -123.012). Sites had no reports of SSWD infection at time of collection. Individuals were inoculated with tissue homogenate prepared from the tube feet, dermal tissue, and coelomic fluid of three P. helianthoides with clinical signs of SSWD. The tissue was ground with a mortar and pestle, then placed in a Stomacher with 10 mL seawater, and centrifuged at 1000 x g for 5 min at 4°C. Organisms injected with the heat-killed homogenate comprised the control group. The treated group were injected with viral-sized fraction homogenate that was syringe-filtered through a 0.22 µm polyethylsulfone filter. Libraries were prepared using the Illumina TruSeq RNA Sample Preparation kit according to the manufacturer’s protocol SRS860068 SAMN03263889 RNA-Seq TRANSCRIPTOMIC PolyA 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 SRX894058 Control_DB SRP051104 Pycnopodia helianthoides (radius 160.5 +/- 30.9 mm) were collected from four sites in Washington State: Langley (LA) (48.038, -122.404); Dabob Bay (DB) (47.813, -122.820); Port Hadlock Marina (PH) (48.030, -122.745); Friday Harbor (FH) (48.545, -123.012). Sites had no reports of SSWD infection at time of collection. Individuals were inoculated with tissue homogenate prepared from the tube feet, dermal tissue, and coelomic fluid of three P. helianthoides with clinical signs of SSWD. The tissue was ground with a mortar and pestle, then placed in a Stomacher with 10 mL seawater, and centrifuged at 1000 x g for 5 min at 4°C. Organisms injected with the heat-killed homogenate comprised the control group. The treated group were injected with viral-sized fraction homogenate that was syringe-filtered through a 0.22 µm polyethylsulfone filter. Libraries were prepared using the Illumina TruSeq RNA Sample Preparation kit according to the manufacturer’s protocol SRS860067 SAMN03263890 RNA-Seq TRANSCRIPTOMIC PolyA 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 SRX894059 Control_FHL SRP051104 Pycnopodia helianthoides (radius 160.5 +/- 30.9 mm) were collected from four sites in Washington State: Langley (LA) (48.038, -122.404); Dabob Bay (DB) (47.813, -122.820); Port Hadlock Marina (PH) (48.030, -122.745); Friday Harbor (FH) (48.545, -123.012). Sites had no reports of SSWD infection at time of collection. Individuals were inoculated with tissue homogenate prepared from the tube feet, dermal tissue, and coelomic fluid of three P. helianthoides with clinical signs of SSWD. The tissue was ground with a mortar and pestle, then placed in a Stomacher with 10 mL seawater, and centrifuged at 1000 x g for 5 min at 4°C. Organisms injected with the heat-killed homogenate comprised the control group. The treated group were injected with viral-sized fraction homogenate that was syringe-filtered through a 0.22 µm polyethylsulfone filter. Libraries were prepared using the Illumina TruSeq RNA Sample Preparation kit according to the manufacturer’s protocol SRS860066 SAMN03263891 RNA-Seq TRANSCRIPTOMIC PolyA 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000