SRX818547 GSM1569554 SRP051380 SRS798601 GSM1569554 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569554 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569554 gds 301569554 GEO Accession GSM1569554 SRX818548 GSM1569555 SRP051380 SRS798602 GSM1569555 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569555 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569555 gds 301569555 GEO Accession GSM1569555 SRX818549 GSM1569556 SRP051380 SRS798603 GSM1569556 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569556 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569556 gds 301569556 GEO Accession GSM1569556 SRX818550 GSM1569557 SRP051380 SRS798604 GSM1569557 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569557 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569557 gds 301569557 GEO Accession GSM1569557 SRX818551 GSM1569558 SRP051380 SRS798605 GSM1569558 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569558 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569558 gds 301569558 GEO Accession GSM1569558 SRX818552 GSM1569559 SRP051380 SRS798606 GSM1569559 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569559 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569559 gds 301569559 GEO Accession GSM1569559 SRX818553 GSM1569560 SRP051380 SRS798607 GSM1569560 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569560 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569560 gds 301569560 GEO Accession GSM1569560 SRX818554 GSM1569561 SRP051380 SRS798608 GSM1569561 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569561 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569561 gds 301569561 GEO Accession GSM1569561 SRX818555 GSM1569562 SRP051380 SRS798609 GSM1569562 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569562 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569562 gds 301569562 GEO Accession GSM1569562 SRX818556 GSM1569563 SRP051380 SRS798610 GSM1569563 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569563 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569563 gds 301569563 GEO Accession GSM1569563 SRX818557 GSM1569564 SRP051380 SRS798611 GSM1569564 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569564 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569564 gds 301569564 GEO Accession GSM1569564 SRX818558 GSM1569565 SRP051380 SRS798612 GSM1569565 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569565 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569565 gds 301569565 GEO Accession GSM1569565 SRX818559 GSM1569566 SRP051380 SRS798613 GSM1569566 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569566 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569566 gds 301569566 GEO Accession GSM1569566 SRX818560 GSM1569567 SRP051380 SRS798614 GSM1569567 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569567 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569567 gds 301569567 GEO Accession GSM1569567 SRX818561 GSM1569568 SRP051380 SRS798615 GSM1569568 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569568 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569568 gds 301569568 GEO Accession GSM1569568 SRX818562 GSM1569569 SRP051380 SRS798616 GSM1569569 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569569 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569569 gds 301569569 GEO Accession GSM1569569 SRX818563 GSM1569570 SRP051380 SRS798617 GSM1569570 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569570 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569570 gds 301569570 GEO Accession GSM1569570 SRX818564 GSM1569571 SRP051380 SRS798618 GSM1569571 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569571 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569571 gds 301569571 GEO Accession GSM1569571 SRX818565 GSM1569572 SRP051380 SRS798619 GSM1569572 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569572 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569572 gds 301569572 GEO Accession GSM1569572 SRX818566 GSM1569573 SRP051380 SRS798620 GSM1569573 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569573 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569573 gds 301569573 GEO Accession GSM1569573 SRX818567 GSM1569574 SRP051380 SRS798621 GSM1569574 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569574 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569574 gds 301569574 GEO Accession GSM1569574 SRX818568 GSM1569575 SRP051380 SRS798622 GSM1569575 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569575 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569575 gds 301569575 GEO Accession GSM1569575 SRX818569 GSM1569576 SRP051380 SRS798623 GSM1569576 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569576 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569576 gds 301569576 GEO Accession GSM1569576 SRX818570 GSM1569577 SRP051380 SRS798624 GSM1569577 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569577 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569577 gds 301569577 GEO Accession GSM1569577 SRX818571 GSM1569578 SRP051380 SRS798625 GSM1569578 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569578 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569578 gds 301569578 GEO Accession GSM1569578 SRX818572 GSM1569579 SRP051380 SRS798626 GSM1569579 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569579 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569579 gds 301569579 GEO Accession GSM1569579 SRX818573 GSM1569580 SRP051380 SRS798627 GSM1569580 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569580 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569580 gds 301569580 GEO Accession GSM1569580 SRX818574 GSM1569581 SRP051380 SRS798628 GSM1569581 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569581 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569581 gds 301569581 GEO Accession GSM1569581 SRX818575 GSM1569582 SRP051380 SRS798629 GSM1569582 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569582 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569582 gds 301569582 GEO Accession GSM1569582 SRX818576 GSM1569583 SRP051380 SRS798630 GSM1569583 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569583 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569583 gds 301569583 GEO Accession GSM1569583 SRX818577 GSM1569584 SRP051380 SRS798631 GSM1569584 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569584 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569584 gds 301569584 GEO Accession GSM1569584 SRX818578 GSM1569585 SRP051380 SRS798632 GSM1569585 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569585 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569585 gds 301569585 GEO Accession GSM1569585 SRX818579 GSM1569586 SRP051380 SRS798633 GSM1569586 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569586 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569586 gds 301569586 GEO Accession GSM1569586 SRX818580 GSM1569587 SRP051380 SRS798634 GSM1569587 ChIP-Seq GENOMIC ChIP Total RNA was extracted by using Qiagen RNA extraction kit according to manufacturer’s manual, and then 10ug total RNA was used for downstream library construction. For ChIP asssy, approximately 1×107 cells were fixed with 1% formaldehyde and quenched by glycine. The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100). DNA was digested to 150-300 bp by MNase (SIGMA) before extensive centrifugation. Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS) was added to the supernatant. The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night. The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 20 µg/ml proteinase K). The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (TIANGEN). RNA-Seq library construction was performed by using Illumina TruSeq kit, briefly, 10ug total RNA as initiation, and then mRNA was purification by oligo-dT magnetic beads. mRNA was fragmented into 200-400bp and revers transcript into dscDNA for library construction. For ChIP-Seq library construction, cross-linked chromatin was fragmented into 150-300bp, and then fragmented DNA was used for library construction and sequencing by Illumina Hiseq2000. Illumina HiSeq 2000 GEO Sample GSM1569587 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1569587 gds 301569587 GEO Accession GSM1569587