SRX833701
20150108
16S rDNA diversity in phyllosphere
SRP051798
SRS811894
CLONE
METAGENOMIC
PCR
600
0
Application Read
Forward
1
1
Application Read
Reverse
301
Illumina MiSeq
SRX834707
20150109
Genetic Diversity and Abundance of Nitrogenase (nifH) and 16S rDNA Gene in the Phyllosphere Samples of an Orchard in North China
SRP051798
PRJNA271827
16S rDNA were amplified using specific primers 515F(5’-GTGCCAGCMGCCGCGG-3’) and 907R (5’-CCGTCAATTCMTTTRAGTTT-3’), targeting the V4-V5 hypervariable regions (392bp)[49]. PCR was carried out with the following cycle conditions: denaturation at 95 °C for 3 min, 27 cycles of 30 s at 95 °C, 30 s at 55 °C and 45 s at 72 °C, then 72 °C for 10 min. The reaction system was composed of 4 µL 5×FastPfu Buffer, 2 µL 2.5 mM dNTPs, 0.8 µL of both forward and reverse primer, 0.4 µL FastPfu Polymerase, 10 ng template and Nuclease-Free Water to a final volume of 20 µL. Amplicons were purified using AxyPrepDNA Gel Extraction Kit (AXYGEN, USA), and quantified using a fluorometric kit (QuantIT PicoGreen, Invitrogen). The purified amplicons were then pooled in equimolar and subject to Illumuna MiSeq sequencing.
SRS812708
SAMN03278123
X
CLONE
METAGENOMIC
PCR
600
0
Application Read
Forward
1
1
Application Read
Reverse
301
Illumina MiSeq
SRX834709
201501092
Genetic Diversity and Abundance of nifH and 16S rDNA in the Phyllosphere of Pyrus sorotina, Prunus armeniaca, Prunus avium and Vitis vinifera
SRP051798
16S rDNA were amplified using specific primers 515F(5’-GTGCCAGCMGCCGCGG-3’) and 907R (5’-CCGTCAATTCMTTTRAGTTT-3’), targeting the V4-V5 hypervariable regions (392bp)[49]. PCR was carried out with the following cycle conditions: denaturation at 95 °C for 3 min, 27 cycles of 30 s at 95 °C, 30 s at 55 °C and 45 s at 72 °C, then 72 °C for 10 min. The reaction system was composed of 4 µL 5×FastPfu Buffer, 2 µL 2.5 mM dNTPs, 0.8 µL of both forward and reverse primer, 0.4 µL FastPfu Polymerase, 10 ng template and Nuclease-Free Water to a final volume of 20 µL. Amplicons were purified using AxyPrepDNA Gel Extraction Kit (AXYGEN, USA), and quantified using a fluorometric kit (QuantIT PicoGreen, Invitrogen). The purified amplicons were then pooled in equimolar and subject to Illumuna MiSeq sequencing.
SRS812710
SAMN03278125
P
CLONE
METAGENOMIC
PCR
600
0
Application Read
Forward
1
1
Application Read
Reverse
301
Illumina MiSeq
SRX834710
201501093
Genetic Diversity and Abundance of nifH and 16S rDNA in the Phyllosphere of Pyrus sorotina, Prunus armeniaca, Prunus avium and Vitis vinifera
SRP051798
PRJNA271827
16S rDNA were amplified using specific primers 515F(5’-GTGCCAGCMGCCGCGG-3’) and 907R (5’-CCGTCAATTCMTTTRAGTTT-3’), targeting the V4-V5 hypervariable regions (392bp)[49]. PCR was carried out with the following cycle conditions: denaturation at 95 °C for 3 min, 27 cycles of 30 s at 95 °C, 30 s at 55 °C and 45 s at 72 °C, then 72 °C for 10 min. The reaction system was composed of 4 µL 5×FastPfu Buffer, 2 µL 2.5 mM dNTPs, 0.8 µL of both forward and reverse primer, 0.4 µL FastPfu Polymerase, 10 ng template and Nuclease-Free Water to a final volume of 20 µL. Amplicons were purified using AxyPrepDNA Gel Extraction Kit (AXYGEN, USA), and quantified using a fluorometric kit (QuantIT PicoGreen, Invitrogen). The purified amplicons were then pooled in equimolar and subject to Illumuna MiSeq sequencing.
SRS812709
SAMN03278124
Y
CLONE
METAGENOMIC
PCR
600
0
Application Read
Forward
1
1
Application Read
Reverse
301
Illumina MiSeq