<?xml version="1.0" encoding="UTF-8"?>
<SAMPLE_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <SAMPLE alias="L.klu_aerobic_diauxic_anaerobic_transcriptome" accession="SRS823853">
    <IDENTIFIERS>
      <PRIMARY_ID>SRS823853</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioSample">SAMN03288813</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>Lachancea kluyveri transcriptome of ancestor and evolved strain in aerobic, post-diauxic and anerobic conditions</TITLE>
    <SAMPLE_NAME>
      <TAXON_ID>4934</TAXON_ID>
      <SCIENTIFIC_NAME>Saccharomyces kluyveri</SCIENTIFIC_NAME>
    </SAMPLE_NAME>
    <DESCRIPTION>A single colony from L. kluyveri (CBS 3082) was evolved by a long-term modified serial dilution transfer method (batch cultures) method in which bacteria were sequentially introduced to compete with yeasts under aerobic conditions. Both evolved and the ancestral strains were grown in duplicates in batch cultivation with synthetic minimal media. Batch cultures of evolved and ancestor were sampled for RNA extraction under both aerobic, post-diauxic and anaerobic conditions in fermeters to determine changes in expression of genes responsible for carbon utilization in the evolved strain. RNA was extracted from these samples. The cDNA libraries were constructed. Paired-end sequencing was performed on Illumina HiSeq2000.</DESCRIPTION>
    <SAMPLE_LINKS>
      <SAMPLE_LINK>
        <XREF_LINK>
          <DB>bioproject</DB>
          <ID>272976</ID>
          <LABEL>PRJNA272976</LABEL>
        </XREF_LINK>
      </SAMPLE_LINK>
    </SAMPLE_LINKS>
    <SAMPLE_ATTRIBUTES>
      <SAMPLE_ATTRIBUTE>
        <TAG>strain</TAG>
        <VALUE>CBS 3082</VALUE>
      </SAMPLE_ATTRIBUTE>
      <SAMPLE_ATTRIBUTE>
        <TAG>host</TAG>
        <VALUE>intestinal canal of Drosophila pinicola</VALUE>
      </SAMPLE_ATTRIBUTE>
      <SAMPLE_ATTRIBUTE>
        <TAG>collection_date</TAG>
        <VALUE>not applicable</VALUE>
      </SAMPLE_ATTRIBUTE>
      <SAMPLE_ATTRIBUTE>
        <TAG>geo_loc_name</TAG>
        <VALUE>Sweden: Lund</VALUE>
      </SAMPLE_ATTRIBUTE>
      <SAMPLE_ATTRIBUTE>
        <TAG>sample_type</TAG>
        <VALUE>Cell culture</VALUE>
      </SAMPLE_ATTRIBUTE>
      <SAMPLE_ATTRIBUTE>
        <TAG>type-material</TAG>
        <VALUE>type material of Saccharomyces kluyveri</VALUE>
      </SAMPLE_ATTRIBUTE>
      <SAMPLE_ATTRIBUTE>
        <TAG>BioSampleModel</TAG>
        <VALUE>Microbe, viral or environmental</VALUE>
      </SAMPLE_ATTRIBUTE>
    </SAMPLE_ATTRIBUTES>
  </SAMPLE>
</SAMPLE_SET>
