SRX890258 Field1 SRP055488 Springtails were sampled from a field location in Zaandam, the Netherlands, and transferred to standardized culturing conditions in the lab. After four months, DNA was isolated from four replicate samples, each consisting of ten animals, and used for targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS857077 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX890264 Field2 SRP055488 Springtails were sampled from a field location in Zaandam, the Netherlands, and transferred to standardized culturing conditions in the lab. After four months, DNA was isolated from four replicate samples, each consisting of ten animals, and used for targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS857077 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX890265 Field 3 SRP055488 Springtails were sampled from a field location in Zaandam, the Netherlands, and transferred to standardized culturing conditions in the lab. After four months, DNA was isolated from four replicate samples, each consisting of ten animals, and used for targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS857077 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX890267 Field 4 SRP055488 Springtails were sampled from a field location in Zaandam, the Netherlands, and transferred to standardized culturing conditions in the lab. After four months, DNA was isolated from four replicate samples, each consisting of ten animals, and used for targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS857077 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX890270 Lab 1 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS857085 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX890339 Lab 2 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS857085 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX890370 Lab 3 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS857085 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX890393 Lab 4 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS857085 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX892839 Suppression 1 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. Each sample was amplified using two different approaches, “Suppression” and “Control”. The Suppression treatment consisted of a nested PCR approach to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS858980 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX892840 Suppression 2 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. Each sample was amplified using two different approaches, “Suppression” and “Control”. The Suppression treatment consisted of a nested PCR approach to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS858980 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX892842 Suppression 3 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. Each sample was amplified using two different approaches, “Suppression” and “Control”. The Suppression treatment consisted of a nested PCR approach to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS858980 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX892845 Suppression 4 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. Each sample was amplified using two different approaches, “Suppression” and “Control”. The Suppression treatment consisted of a nested PCR approach to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS858980 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX892847 Control 1 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. Each sample was amplified using two different approaches, “Suppression” and “Control”. The Control treatment consisted of a direct PCR with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS858985 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX892850 Control 2 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. Each sample was amplified using two different approaches, “Suppression” and “Control”. The Control treatment consisted of a direct PCR with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS858985 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX892858 Control 3 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. Each sample was amplified using two different approaches, “Suppression” and “Control”. The Control treatment consisted of a direct PCR with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS858985 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX892860 Control 4 SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. Each sample was amplified using two different approaches, “Suppression” and “Control”. The Control treatment consisted of a direct PCR with primers 357F and 518R, with appropriate barcodes and Illumina linkers. SRS858985 16S V3 AMPLICON METAGENOMIC PCR 302 0 Application Read Forward 1 1 Application Read Reverse 152 Illumina MiSeq SRX951291 Field1-Sanger SRP055488 Springtails were sampled from a field location in Zaandam, the Netherlands, and transferred to standardized culturing conditions in the lab. After four months, DNA was isolated from four replicate samples, each consisting of ten animals, and used for targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 1392R. The DNA fragments were then purified, inserted into plasmid pGEM-T and transformed into Escherichia coli XL-1 Blue. Positive transformants were screened by PCR to verify the insertion of the fragment and then subjected to RFLP analysis. Six to eight clones showing different RFLP patterns were chosen for sequencing. SRS857077 16S V3/V8 CLONE METAGENOMIC PCR 0 0 Application Read Forward 1 AB 3730xL Genetic Analyzer SRX951292 Field2-Sanger SRP055488 Springtails were sampled from a field location in Zaandam, the Netherlands, and transferred to standardized culturing conditions in the lab. After four months, DNA was isolated from four replicate samples, each consisting of ten animals, and used for targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 1392R. The DNA fragments were then purified, inserted into plasmid pGEM-T and transformed into Escherichia coli XL-1 Blue. Positive transformants were screened by PCR to verify the insertion of the fragment and then subjected to RFLP analysis. Six to eight clones showing different RFLP patterns were chosen for sequencing. SRS857077 16S V3/V8 CLONE METAGENOMIC PCR 0 0 Application Read Forward 1 AB 3730xL Genetic Analyzer SRX951293 Field3-Sanger SRP055488 Springtails were sampled from a field location in Zaandam, the Netherlands, and transferred to standardized culturing conditions in the lab. After four months, DNA was isolated from four replicate samples, each consisting of ten animals, and used for targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 1392R. The DNA fragments were then purified, inserted into plasmid pGEM-T and transformed into Escherichia coli XL-1 Blue. Positive transformants were screened by PCR to verify the insertion of the fragment and then subjected to RFLP analysis. Six to eight clones showing different RFLP patterns were chosen for sequencing. SRS857077 16S V3/V8 CLONE METAGENOMIC PCR 0 0 Application Read Forward 1 AB 3730xL Genetic Analyzer SRX951294 Field4-Sanger SRP055488 Springtails were sampled from a field location in Zaandam, the Netherlands, and transferred to standardized culturing conditions in the lab. After four months, DNA was isolated from four replicate samples, each consisting of ten animals, and used for targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 1392R. The DNA fragments were then purified, inserted into plasmid pGEM-T and transformed into Escherichia coli XL-1 Blue. Positive transformants were screened by PCR to verify the insertion of the fragment and then subjected to RFLP analysis. Six to eight clones showing different RFLP patterns were chosen for sequencing. SRS857077 16S V3/V8 CLONE METAGENOMIC PCR 0 0 Application Read Forward 1 AB 3730xL Genetic Analyzer SRX951295 Lab1-Sanger SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 1392R. The DNA fragments were then purified, inserted into plasmid pGEM-T and transformed into Escherichia coli XL-1 Blue. Positive transformants were screened by PCR to verify the insertion of the fragment and then subjected to RFLP analysis. Six to eight clones showing different RFLP patterns were chosen for sequencing. SRS857085 16S V3/V8 CLONE METAGENOMIC PCR 0 0 Application Read Forward 1 AB 3730xL Genetic Analyzer SRX951296 Lab2-Sanger SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 1392R. The DNA fragments were then purified, inserted into plasmid pGEM-T and transformed into Escherichia coli XL-1 Blue. Positive transformants were screened by PCR to verify the insertion of the fragment and then subjected to RFLP analysis. Six to eight clones showing different RFLP patterns were chosen for sequencing. SRS857085 16S V3/V8 CLONE METAGENOMIC PCR 0 0 Application Read Forward 1 AB 3730xL Genetic Analyzer SRX951298 Lab3-Sanger SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 1392R. The DNA fragments were then purified, inserted into plasmid pGEM-T and transformed into Escherichia coli XL-1 Blue. Positive transformants were screened by PCR to verify the insertion of the fragment and then subjected to RFLP analysis. Six to eight clones showing different RFLP patterns were chosen for sequencing. SRS857085 16S V3/V8 CLONE METAGENOMIC PCR 0 0 Application Read Forward 1 AB 3730xL Genetic Analyzer SRX951299 Lab4-Sanger SRP055488 Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 1392R. The DNA fragments were then purified, inserted into plasmid pGEM-T and transformed into Escherichia coli XL-1 Blue. Positive transformants were screened by PCR to verify the insertion of the fragment and then subjected to RFLP analysis. Six to eight clones showing different RFLP patterns were chosen for sequencing. SRS857085 16S V3/V8 CLONE METAGENOMIC PCR 0 0 Application Read Forward 1 AB 3730xL Genetic Analyzer