SRX980531 D.gigas Beak Transcriptome SRP017243 PRJNA181271 RNA-Seq analysis was performed as previously described17,47. Briefly, total RNA from a D. gigas buccal mass (tissue that was in close proximity to the upper and lower beak were combined and used) was extracted with a Qiagen RNeasy mini kit. Poly-A mRNA was then enriched from 10 µg of total RNA with oligo dT beads (Invitrogen). The buccal mass sample was collected on R/V New Horizon (Scripps Institute of Oceanography, San Diego, CA, USA), June 2010, at Guayamas Basin, Mexico, with Permit No. DAPA/2/100510/1640. ~100 ng of recovered poly-A mRNA was used to construct an RNA-seq library (ScriptSeqTM mRNA-Seq library kit v1, Epicenter, Illumina) according to manufacturer’s instructions. Phusion PCR polymerase (Thermo Scientific) was used for the final library amplification (12 cycles). PCR cleanup was performed with the MinElute PCR purification kit (Qiagen) and the library quality was assessed with an Agilent 21000 Bioanalyzer. The library was then sequenced on an Illumina GA IIx and 2 lanes of 2x76 bp paired-end-reads were collected. SRS895470 RNA-Seq TRANSCRIPTOMIC PolyA 152 0 Application Read Forward 1 1 Application Read Reverse 77 Illumina Genome Analyzer IIx Trinity r2013-06-08