SRX980997 Psp_24MN13_PGM_reads SRP055906 A 400 bp size fractionated library was constructed using Ion Plus Fragment Library Kit, templates with the Ion OT2 400 bp Template Kit, and sequenced on a 318v2 chip using Ion 400 bp Sequencing kit. SRS895858 WGS GENOMIC RANDOM 0 0 Technical Read Adapter 1 1 Application Read Forward 5 Ion Torrent PGM SRX980998 Psp_24MN13_Illumina_reads SRP055906 Library was constructing using NEBNext library prep, cleaned up with QIAQuick purification kit, and size fractionated using E-Gel size selection system. SRS895858 WGS GENOMIC RANDOM 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina Genome Analyzer IIx SRX982204 Psp24MN13_RNA SRP055906 For the purpose of improving gene prediction, we generated RNA-seq data using the Ion Torrent PGM. Total RNA was isolated from liquid shaking culture using TriZol, treated with DNaseI, and poly-adenelated RNA was selected using a Dynabeads mRNA purification kit. Sequencing libraries were constructed from mRNA using Ion Total RNA-Seq v2 kit and multiplexed on a 318v2 chip on the PGM. SRS895858 RNA-Seq TRANSCRIPTOMIC size fractionation 0 0 Technical Read Adapter 1 1 Application Read Forward 5 Ion Torrent PGM