<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT alias="PBMC rep2" accession="SRX1002654" center_name="university of surrey">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1002654</PRIMARY_ID>
      <SUBMITTER_ID namespace="university of surrey">PBMC rep2</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>Raw reads for M. tubercuslosis Mariner transposon library passaged though dendritic cell derived from PBMC from donor 2</TITLE>
    <STUDY_REF accession="SRP057496">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP057496</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>M. tuberculosis GC1237 was mutagenised with a Mariner transposon to give a library of 2e5. This was passaged through PBMC derived dendritic cells from donors. Transposon junctions from surviving isolates were amplified and sequenced.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS921067">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS921067</PRIMARY_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME/>
        <LIBRARY_STRATEGY>Tn-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
      <SPOT_DESCRIPTOR>
        <SPOT_DECODE_SPEC>
          <SPOT_LENGTH>101</SPOT_LENGTH>
          <READ_SPEC>
            <READ_INDEX>0</READ_INDEX>
            <READ_CLASS>Application Read</READ_CLASS>
            <READ_TYPE>Forward</READ_TYPE>
            <BASE_COORD>1</BASE_COORD>
          </READ_SPEC>
        </SPOT_DECODE_SPEC>
      </SPOT_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <PROCESSING/>
  </EXPERIMENT>
</EXPERIMENT_SET>
