SRS918866 SAMN03565584 GSM1665397 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name FACS Corin+ cells from ventral midbrain cells from ES cells d14 protocol H9 cells were differentiated towards a ventral midbrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience.In addition, cells were further ventralized by adding Purmophamine to the media (StemGent, 5uM) during day0-11 of differentiation. On day 14 the cells were stained for Corin (R&D 1:200) and FACS sorted. cell line H9 cell type FACS Corin+ cells from ventral midbrain cells from ES cells d14 SRS918872 SAMN03565582 GSM1665395 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name FACS Corin+ cells from ventral midbrain cells from ES cells d14 protocol H9 cells were differentiated towards a ventral midbrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience.In addition, cells were further ventralized by adding Purmophamine to the media (StemGent, 5uM) during day0-11 of differentiation. On day 14 the cells were stained for Corin (R&D 1:200) and FACS sorted. cell line H9 cell type FACS Corin+ cells from ventral midbrain cells from ES cells d14 SRS918874 SAMN03565571 GSM1665393 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name FACS Corin+ cells from ventral midbrain cells from ES cells d14 protocol H9 cells were differentiated towards a ventral midbrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience.In addition, cells were further ventralized by adding Purmophamine to the media (StemGent, 5uM) during day0-11 of differentiation. On day 14 the cells were stained for Corin (R&D 1:200) and FACS sorted. cell line H9 cell type FACS Corin+ cells from ventral midbrain cells from ES cells d14 SRS918875 SAMN03565580 GSM1665392 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name FACS Corin+ cells from ventral midbrain cells from ES cells d14 protocol H9 cells were differentiated towards a ventral midbrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience.In addition, cells were further ventralized by adding Purmophamine to the media (StemGent, 5uM) during day0-11 of differentiation. On day 14 the cells were stained for Corin (R&D 1:200) and FACS sorted. cell line H9 cell type FACS Corin+ cells from ventral midbrain cells from ES cells d14 SRS918877 SAMN03565578 GSM1665390 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated hindbrain cells from ES cells d14 protocol H9 cells were differentiated towards a hindbrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated hindbrain cells from ES cells d14 SRS918878 SAMN03565577 GSM1665389 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated hindbrain cells from ES cells d14 protocol H9 cells were differentiated towards a hindbrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated hindbrain cells from ES cells d14 SRS918880 SAMN03565575 GSM1665387 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated midbrain cells from ES cells d14 protocol H9 cells were differentiated towards midbrain according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated midbrain cells from ES cells d14 SRS918881 SAMN03565574 GSM1665386 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated midbrain cells from ES cells d14 protocol H9 cells were differentiated towards midbrain according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated midbrain cells from ES cells d14 SRS918883 SAMN03565572 GSM1665384 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated midbrain cells from ES cells d14 protocol H9 cells were differentiated towards midbrain according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated midbrain cells from ES cells d14 SRS918884 SAMN03565570 GSM1665383 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated mesenchymal cells from ES cells d14 protocol The media on confluent H9 cells was changed to RPMI (Mediatech) complemented with Pen/strep, glutamate and Activin A (R&D) for one day. On the sencond day the media was further supplemented with fetal bovine serum. cell line H9 cell type Differentiated mesenchymal cells from ES cells d14 SRS918886 SAMN03565568 GSM1665381 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated mesenchymal cells from ES cells d14 protocol The media on confluent H9 cells was changed to RPMI (Mediatech) complemented with Pen/strep, glutamate and Activin A (R&D) for one day. On the sencond day the media was further supplemented with fetal bovine serum. cell line H9 cell type Differentiated mesenchymal cells from ES cells d14 SRS918887 SAMN03565567 GSM1665380 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated mesenchymal cells from ES cells d14 protocol The media on confluent H9 cells was changed to RPMI (Mediatech) complemented with Pen/strep, glutamate and Activin A (R&D) for one day. On the sencond day the media was further supplemented with fetal bovine serum. cell line H9 cell type Differentiated mesenchymal cells from ES cells d14 SRS918889 SAMN03565565 GSM1665378 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated forebrain cells from ES cells d14 protocol H9 cells were differentiated towards a forebrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated forebrain cells from ES cells d14 SRS918890 SAMN03565564 GSM1665377 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated forebrain cells from ES cells d14 protocol H9 cells were differentiated towards a forebrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated forebrain cells from ES cells d14 SRS918892 SAMN03565562 GSM1665375 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Embryonic stem cells protocol H9 cells were expanded and maintained to gamma-irradiated mouse fibroblasts in DMEM/F12, 20%KSR, 0.05 mM 2-merkaptoethanom, 0.5% Pen/Strep, 0.5% glutamine and 10ng/ml bFGF. Cells were passaged once weeksly with 0.5mM EDTA. cell line H9 cell type Embryonic stem cells SRS918893 SAMN03565561 GSM1665374 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Embryonic stem cells protocol H9 cells were expanded and maintained to gamma-irradiated mouse fibroblasts in DMEM/F12, 20%KSR, 0.05 mM 2-merkaptoethanom, 0.5% Pen/Strep, 0.5% glutamine and 10ng/ml bFGF. Cells were passaged once weeksly with 0.5mM EDTA. cell line H9 cell type Embryonic stem cells SRS918895 SAMN03565559 GSM1665372 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Embryonic stem cells protocol H9 cells were expanded and maintained to gamma-irradiated mouse fibroblasts in DMEM/F12, 20%KSR, 0.05 mM 2-merkaptoethanom, 0.5% Pen/Strep, 0.5% glutamine and 10ng/ml bFGF. Cells were passaged once weeksly with 0.5mM EDTA. cell line H9 cell type Embryonic stem cells SRS918896 SAMN03565558 GSM1665371 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Lung fibroblast protocol Human Fetal Lung Fibroblasts (hFL1s) were purchased from American Type Culture Collection (ATCC). Passage 12 – 16 hFL1s were expanded in DMEM supplemented with 10% FBS, 0.5% Pen/Strep, 0.5% glutamine. cell type Lung fibroblast SRS918898 SAMN03565556 GSM1665369 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Lung fibroblast protocol Human Fetal Lung Fibroblasts (hFL1s) were purchased from American Type Culture Collection (ATCC). Passage 12 – 16 hFL1s were expanded in DMEM supplemented with 10% FBS, 0.5% Pen/Strep, 0.5% glutamine. cell type Lung fibroblast SRS918899 SAMN03565555 GSM1665368 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Lung fibroblast protocol Human Fetal Lung Fibroblasts (hFL1s) were purchased from American Type Culture Collection (ATCC). Passage 12 – 16 hFL1s were expanded in DMEM supplemented with 10% FBS, 0.5% Pen/Strep, 0.5% glutamine. cell type Lung fibroblast SRS918901 SAMN03565553 GSM1665366 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Neural stem cells protocol Subdissected human fetal ventral midbrai was plated at 12,500cells/cm2 onto plastic coated with polyornithine, fibornectin and laminin. Neurobasal medium was supplemented with B27, Glutamine, BDNF, GDNF and ascorbic acid and was exchanged every 2-3days. cell type Neural stem cells origin Subdissection from embryonic human tissue SRS918902 SAMN03565552 GSM1665365 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Neural stem cells protocol Subdissected human fetal ventral midbrai was plated at 12,500cells/cm2 onto plastic coated with polyornithine, fibornectin and laminin. Neurobasal medium was supplemented with B27, Glutamine, BDNF, GDNF and ascorbic acid and was exchanged every 2-3days. cell type Neural stem cells origin Subdissection from embryonic human tissue SRS918865 SAMN03565585 GSM1665398 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name FACS Corin+ cells from ventral midbrain cells from ES cells d14 protocol H9 cells were differentiated towards a ventral midbrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience.In addition, cells were further ventralized by adding Purmophamine to the media (StemGent, 5uM) during day0-11 of differentiation. On day 14 the cells were stained for Corin (R&D 1:200) and FACS sorted. cell line H9 cell type FACS Corin+ cells from ventral midbrain cells from ES cells d14 SRS918871 SAMN03565583 GSM1665396 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name FACS Corin+ cells from ventral midbrain cells from ES cells d14 protocol H9 cells were differentiated towards a ventral midbrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience.In addition, cells were further ventralized by adding Purmophamine to the media (StemGent, 5uM) during day0-11 of differentiation. On day 14 the cells were stained for Corin (R&D 1:200) and FACS sorted. cell line H9 cell type FACS Corin+ cells from ventral midbrain cells from ES cells d14 SRS918873 SAMN03565581 GSM1665394 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name FACS Corin+ cells from ventral midbrain cells from ES cells d14 protocol H9 cells were differentiated towards a ventral midbrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience.In addition, cells were further ventralized by adding Purmophamine to the media (StemGent, 5uM) during day0-11 of differentiation. On day 14 the cells were stained for Corin (R&D 1:200) and FACS sorted. cell line H9 cell type FACS Corin+ cells from ventral midbrain cells from ES cells d14 SRS918876 SAMN03565579 GSM1665391 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated hindbrain cells from ES cells d14 protocol H9 cells were differentiated towards a hindbrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated hindbrain cells from ES cells d14 SRS918879 SAMN03565576 GSM1665388 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated hindbrain cells from ES cells d14 protocol H9 cells were differentiated towards a hindbrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated hindbrain cells from ES cells d14 SRS918882 SAMN03565573 GSM1665385 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated midbrain cells from ES cells d14 protocol H9 cells were differentiated towards midbrain according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated midbrain cells from ES cells d14 SRS918885 SAMN03565569 GSM1665382 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated mesenchymal cells from ES cells d14 protocol The media on confluent H9 cells was changed to RPMI (Mediatech) complemented with Pen/strep, glutamate and Activin A (R&D) for one day. On the sencond day the media was further supplemented with fetal bovine serum. cell line H9 cell type Differentiated mesenchymal cells from ES cells d14 SRS918888 SAMN03565566 GSM1665379 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated forebrain cells from ES cells d14 protocol H9 cells were differentiated towards a forebrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated forebrain cells from ES cells d14 SRS918891 SAMN03565563 GSM1665376 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Differentiated forebrain cells from ES cells d14 protocol H9 cells were differentiated towards a forebrain fate according to the protocol in Kirkeby et al 2012, Frontiers in Cellular Neuroscience. cell line H9 cell type Differentiated forebrain cells from ES cells d14 SRS918894 SAMN03565560 GSM1665373 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Embryonic stem cells protocol H9 cells were expanded and maintained to gamma-irradiated mouse fibroblasts in DMEM/F12, 20%KSR, 0.05 mM 2-merkaptoethanom, 0.5% Pen/Strep, 0.5% glutamine and 10ng/ml bFGF. Cells were passaged once weeksly with 0.5mM EDTA. cell line H9 cell type Embryonic stem cells SRS918897 SAMN03565557 GSM1665370 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Lung fibroblast protocol Human Fetal Lung Fibroblasts (hFL1s) were purchased from American Type Culture Collection (ATCC). Passage 12 – 16 hFL1s were expanded in DMEM supplemented with 10% FBS, 0.5% Pen/Strep, 0.5% glutamine. cell type Lung fibroblast SRS918900 SAMN03565554 GSM1665367 9606 Homo sapiens bioproject 282001 PRJNA282001 source_name Neural stem cells protocol Subdissected human fetal ventral midbrai was plated at 12,500cells/cm2 onto plastic coated with polyornithine, fibornectin and laminin. Neurobasal medium was supplemented with B27, Glutamine, BDNF, GDNF and ascorbic acid and was exchanged every 2-3days. cell type Neural stem cells origin Subdissection from embryonic human tissue