SRX1041846 GSM1698086 SRP058819 SRS948188 GSM1698086 RNA-Seq TRANSCRIPTOMIC cDNA RNA from Drosophila S2 cells was extracted using Promega's SV Total RNA Isolation System (Z3100) according to manufacturer’s protocol. The Illumina® mRNA-Seq Sample Prep Kit was used to process the sample(s). The sample preparation was performed according the Illumina protocol "Preparing Samples for Sequencing of mRNA" (1004898 Rev. D). Briefly, mRNA was isolated from total RNA using the poly-T-oligo-attached magnetic beads. After fragementation of the mRNA, a cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplicification of the resulting product. The quality and yield after sample preparation was measured with a DNA 1000 Lab-on-a-Chip (see appendices). The size of the resulting products was consistent with the expected size of approximately 300 bp. Clustering and DNA sequencing using the Illumina HiSeq 2000 (Solexa) were performed according manufacturer’s protocols. A total of 6.5 pmol of DNA was used. Two sequencing reads of 100 cycles each using the Read 1 sequencing and Read 2 sequencing primers were performed with the flow cell. Illumina HiSeq 2000 GEO Sample GSM1698086 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1698086 gds 301698086 GEO Accession GSM1698086 SRX1041847 GSM1698087 SRP058819 SRS948187 GSM1698087 RNA-Seq TRANSCRIPTOMIC cDNA RNA from Drosophila S2 cells was extracted using Promega's SV Total RNA Isolation System (Z3100) according to manufacturer’s protocol. The Illumina® mRNA-Seq Sample Prep Kit was used to process the sample(s). The sample preparation was performed according the Illumina protocol "Preparing Samples for Sequencing of mRNA" (1004898 Rev. D). Briefly, mRNA was isolated from total RNA using the poly-T-oligo-attached magnetic beads. After fragementation of the mRNA, a cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplicification of the resulting product. The quality and yield after sample preparation was measured with a DNA 1000 Lab-on-a-Chip (see appendices). The size of the resulting products was consistent with the expected size of approximately 300 bp. Clustering and DNA sequencing using the Illumina HiSeq 2000 (Solexa) were performed according manufacturer’s protocols. A total of 6.5 pmol of DNA was used. Two sequencing reads of 100 cycles each using the Read 1 sequencing and Read 2 sequencing primers were performed with the flow cell. Illumina HiSeq 2000 GEO Sample GSM1698087 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1698087 gds 301698087 GEO Accession GSM1698087 SRX1041848 GSM1698088 SRP058819 SRS948186 GSM1698088 RNA-Seq TRANSCRIPTOMIC cDNA RNA from Drosophila S2 cells was extracted using Promega's SV Total RNA Isolation System (Z3100) according to manufacturer’s protocol. The Illumina® mRNA-Seq Sample Prep Kit was used to process the sample(s). The sample preparation was performed according the Illumina protocol "Preparing Samples for Sequencing of mRNA" (1004898 Rev. D). Briefly, mRNA was isolated from total RNA using the poly-T-oligo-attached magnetic beads. After fragementation of the mRNA, a cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplicification of the resulting product. The quality and yield after sample preparation was measured with a DNA 1000 Lab-on-a-Chip (see appendices). The size of the resulting products was consistent with the expected size of approximately 300 bp. Clustering and DNA sequencing using the Illumina HiSeq 2000 (Solexa) were performed according manufacturer’s protocols. A total of 6.5 pmol of DNA was used. Two sequencing reads of 100 cycles each using the Read 1 sequencing and Read 2 sequencing primers were performed with the flow cell. Illumina HiSeq 2000 GEO Sample GSM1698088 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1698088 gds 301698088 GEO Accession GSM1698088 SRX1041849 GSM1698089 SRP058819 SRS948185 GSM1698089 RNA-Seq TRANSCRIPTOMIC cDNA RNA from Drosophila S2 cells was extracted using Promega's SV Total RNA Isolation System (Z3100) according to manufacturer’s protocol. The Illumina® mRNA-Seq Sample Prep Kit was used to process the sample(s). The sample preparation was performed according the Illumina protocol "Preparing Samples for Sequencing of mRNA" (1004898 Rev. D). Briefly, mRNA was isolated from total RNA using the poly-T-oligo-attached magnetic beads. After fragementation of the mRNA, a cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplicification of the resulting product. The quality and yield after sample preparation was measured with a DNA 1000 Lab-on-a-Chip (see appendices). The size of the resulting products was consistent with the expected size of approximately 300 bp. Clustering and DNA sequencing using the Illumina HiSeq 2000 (Solexa) were performed according manufacturer’s protocols. A total of 6.5 pmol of DNA was used. Two sequencing reads of 100 cycles each using the Read 1 sequencing and Read 2 sequencing primers were performed with the flow cell. Illumina HiSeq 2000 GEO Sample GSM1698089 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1698089 gds 301698089 GEO Accession GSM1698089