SRX1045004 GSM1701104 SRP058941 SRS950349 GSM1701104 RNA-Seq TRANSCRIPTOMIC cDNA commerically available Human Testes Total RNA - Ambion AM7972 Sperm RNA was isolated and purified using a modified RNeasy (Qiagen) protocol and finally the RNA quality assessed for DNA contamination as described in paper A total of 2 ng of unfractionated input RNA was reverse transcribed and the resulting cDNA amplified using SeqPlex RNA Amplification (Sigma-Aldrich Co.) allowing for the use of samples with low quantity of spermatozoa Amplified primer dimers were removed prior to library construction using Agencourt AMPure XP (Beckman Coulter) and libraries were constructed with 50 ng of amplified cDNA using Encore NGS Library System I (Nugen, Inc.). 4bp inline Illumina index primers allowed for multiplexing of samples Paired-end sequencing (2x51 + 6Index) was performed using an Illumina HiSeq 2500 sequencer Illumina HiSeq 2500 GEO Sample GSM1701104 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1701104 gds 301701104 GEO Accession GSM1701104 SRX1045005 GSM1701105 SRP058941 SRS950348 GSM1701105 RNA-Seq TRANSCRIPTOMIC cDNA commerically available Human Testes Total RNA - Ambion AM7972 Sperm RNA was isolated and purified using a modified RNeasy (Qiagen) protocol and finally the RNA quality assessed for DNA contamination as described in paper A total of 2 ng of unfractionated input RNA was reverse transcribed and the resulting cDNA amplified using SeqPlex RNA Amplification (Sigma-Aldrich Co.) allowing for the use of samples with low quantity of spermatozoa Amplified primer dimers were removed prior to library construction using Agencourt AMPure XP (Beckman Coulter) and libraries were constructed with 50 ng of amplified cDNA using Encore NGS Library System I (Nugen, Inc.). 4bp inline Illumina index primers allowed for multiplexing of samples Paired-end sequencing (2x51 + 6Index) was performed using an Illumina HiSeq 2500 sequencer Illumina HiSeq 2500 GEO Sample GSM1701105 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1701105 gds 301701105 GEO Accession GSM1701105