<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT alias="GSM1855720" accession="SRX1155632">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1155632</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM1855720</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM1855720: WT_P1; Fusarium graminearum PH-1; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP062498" refname="GSE72124">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP062498</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS1037829">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1037829</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM1855720</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>RNA isolation was performed using RNeasy Plant Mini Kit (Qiagen, 74904) following the instructions of the manufacturer with one modification and one extension: Instead of eluting RNA once in 30 µL RNase- free water, elution was performed in 2x 20 µL RNase- free water with a 3 minute standing period at room temperature after each water application to the column. Additionally on-column DNase digestion was done during the extraction protocol by using RNase-Free DNase Set (Qiagen, 79254). RNA quantity was first checked on the NanoDrop 2000c Spectrophotometer (Thermo Scientific). Afterwards RNA quantity and integrity was validated by using the Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer machine following the instructions of the provider. The cDNA Synthesis was done with the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, # K1632) using random hexamer primer and following the manufacturer´s protocol. Illumina sequencing libraries were made from RNA samples according to TruSeq RNA Sample prep kit v2 (Illumina) following the manufacturers protocol with 1µg total RNA input. 50 bp single end sequencing was performed using a HiSeq Illumina sequencer. Obtained sequences were de-multiplexed, quality controlled and mapped on the Aspergillus nidulans genome assembly (A_nidulans_FGSC_A4_version_s10-m03-r07).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>301855720</ID>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM1855720</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM1855721" accession="SRX1155633">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1155633</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM1855721</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM1855721: WT_P2; Fusarium graminearum PH-1; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP062498" refname="GSE72124">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP062498</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS1037828">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1037828</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM1855721</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>RNA isolation was performed using RNeasy Plant Mini Kit (Qiagen, 74904) following the instructions of the manufacturer with one modification and one extension: Instead of eluting RNA once in 30 µL RNase- free water, elution was performed in 2x 20 µL RNase- free water with a 3 minute standing period at room temperature after each water application to the column. Additionally on-column DNase digestion was done during the extraction protocol by using RNase-Free DNase Set (Qiagen, 79254). RNA quantity was first checked on the NanoDrop 2000c Spectrophotometer (Thermo Scientific). Afterwards RNA quantity and integrity was validated by using the Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer machine following the instructions of the provider. The cDNA Synthesis was done with the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, # K1632) using random hexamer primer and following the manufacturer´s protocol. Illumina sequencing libraries were made from RNA samples according to TruSeq RNA Sample prep kit v2 (Illumina) following the manufacturers protocol with 1µg total RNA input. 50 bp single end sequencing was performed using a HiSeq Illumina sequencer. Obtained sequences were de-multiplexed, quality controlled and mapped on the Aspergillus nidulans genome assembly (A_nidulans_FGSC_A4_version_s10-m03-r07).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>301855721</ID>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM1855721</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM1855722" accession="SRX1155634">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1155634</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM1855722</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM1855722: WT_P3; Fusarium graminearum PH-1; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP062498" refname="GSE72124">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP062498</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS1037826">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1037826</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM1855722</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>RNA isolation was performed using RNeasy Plant Mini Kit (Qiagen, 74904) following the instructions of the manufacturer with one modification and one extension: Instead of eluting RNA once in 30 µL RNase- free water, elution was performed in 2x 20 µL RNase- free water with a 3 minute standing period at room temperature after each water application to the column. Additionally on-column DNase digestion was done during the extraction protocol by using RNase-Free DNase Set (Qiagen, 79254). RNA quantity was first checked on the NanoDrop 2000c Spectrophotometer (Thermo Scientific). Afterwards RNA quantity and integrity was validated by using the Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer machine following the instructions of the provider. The cDNA Synthesis was done with the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, # K1632) using random hexamer primer and following the manufacturer´s protocol. Illumina sequencing libraries were made from RNA samples according to TruSeq RNA Sample prep kit v2 (Illumina) following the manufacturers protocol with 1µg total RNA input. 50 bp single end sequencing was performed using a HiSeq Illumina sequencer. Obtained sequences were de-multiplexed, quality controlled and mapped on the Aspergillus nidulans genome assembly (A_nidulans_FGSC_A4_version_s10-m03-r07).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>301855722</ID>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM1855722</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM1855723" accession="SRX1155635">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1155635</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM1855723</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM1855723: WT_S1; Fusarium graminearum PH-1; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP062498" refname="GSE72124">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP062498</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS1037827">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1037827</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM1855723</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>RNA isolation was performed using RNeasy Plant Mini Kit (Qiagen, 74904) following the instructions of the manufacturer with one modification and one extension: Instead of eluting RNA once in 30 µL RNase- free water, elution was performed in 2x 20 µL RNase- free water with a 3 minute standing period at room temperature after each water application to the column. Additionally on-column DNase digestion was done during the extraction protocol by using RNase-Free DNase Set (Qiagen, 79254). RNA quantity was first checked on the NanoDrop 2000c Spectrophotometer (Thermo Scientific). Afterwards RNA quantity and integrity was validated by using the Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer machine following the instructions of the provider. The cDNA Synthesis was done with the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, # K1632) using random hexamer primer and following the manufacturer´s protocol. Illumina sequencing libraries were made from RNA samples according to TruSeq RNA Sample prep kit v2 (Illumina) following the manufacturers protocol with 1µg total RNA input. 50 bp single end sequencing was performed using a HiSeq Illumina sequencer. Obtained sequences were de-multiplexed, quality controlled and mapped on the Aspergillus nidulans genome assembly (A_nidulans_FGSC_A4_version_s10-m03-r07).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>301855723</ID>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM1855723</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM1855724" accession="SRX1155636">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1155636</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM1855724</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM1855724: WT_S2; Fusarium graminearum PH-1; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP062498" refname="GSE72124">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP062498</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS1037825">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1037825</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM1855724</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>RNA isolation was performed using RNeasy Plant Mini Kit (Qiagen, 74904) following the instructions of the manufacturer with one modification and one extension: Instead of eluting RNA once in 30 µL RNase- free water, elution was performed in 2x 20 µL RNase- free water with a 3 minute standing period at room temperature after each water application to the column. Additionally on-column DNase digestion was done during the extraction protocol by using RNase-Free DNase Set (Qiagen, 79254). RNA quantity was first checked on the NanoDrop 2000c Spectrophotometer (Thermo Scientific). Afterwards RNA quantity and integrity was validated by using the Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer machine following the instructions of the provider. The cDNA Synthesis was done with the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, # K1632) using random hexamer primer and following the manufacturer´s protocol. Illumina sequencing libraries were made from RNA samples according to TruSeq RNA Sample prep kit v2 (Illumina) following the manufacturers protocol with 1µg total RNA input. 50 bp single end sequencing was performed using a HiSeq Illumina sequencer. Obtained sequences were de-multiplexed, quality controlled and mapped on the Aspergillus nidulans genome assembly (A_nidulans_FGSC_A4_version_s10-m03-r07).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>301855724</ID>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM1855724</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM1855725" accession="SRX1155637">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1155637</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM1855725</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM1855725: WT_S3; Fusarium graminearum PH-1; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP062498" refname="GSE72124">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP062498</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS1037824">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1037824</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM1855725</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>RNA isolation was performed using RNeasy Plant Mini Kit (Qiagen, 74904) following the instructions of the manufacturer with one modification and one extension: Instead of eluting RNA once in 30 µL RNase- free water, elution was performed in 2x 20 µL RNase- free water with a 3 minute standing period at room temperature after each water application to the column. Additionally on-column DNase digestion was done during the extraction protocol by using RNase-Free DNase Set (Qiagen, 79254). RNA quantity was first checked on the NanoDrop 2000c Spectrophotometer (Thermo Scientific). Afterwards RNA quantity and integrity was validated by using the Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer machine following the instructions of the provider. The cDNA Synthesis was done with the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, # K1632) using random hexamer primer and following the manufacturer´s protocol. Illumina sequencing libraries were made from RNA samples according to TruSeq RNA Sample prep kit v2 (Illumina) following the manufacturers protocol with 1µg total RNA input. 50 bp single end sequencing was performed using a HiSeq Illumina sequencer. Obtained sequences were de-multiplexed, quality controlled and mapped on the Aspergillus nidulans genome assembly (A_nidulans_FGSC_A4_version_s10-m03-r07).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>301855725</ID>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM1855725</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM1855726" accession="SRX1155638">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1155638</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM1855726</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM1855726: WT_A1; Fusarium graminearum PH-1; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP062498" refname="GSE72124">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP062498</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS1037822">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1037822</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM1855726</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>RNA isolation was performed using RNeasy Plant Mini Kit (Qiagen, 74904) following the instructions of the manufacturer with one modification and one extension: Instead of eluting RNA once in 30 µL RNase- free water, elution was performed in 2x 20 µL RNase- free water with a 3 minute standing period at room temperature after each water application to the column. Additionally on-column DNase digestion was done during the extraction protocol by using RNase-Free DNase Set (Qiagen, 79254). RNA quantity was first checked on the NanoDrop 2000c Spectrophotometer (Thermo Scientific). Afterwards RNA quantity and integrity was validated by using the Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer machine following the instructions of the provider. The cDNA Synthesis was done with the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, # K1632) using random hexamer primer and following the manufacturer´s protocol. Illumina sequencing libraries were made from RNA samples according to TruSeq RNA Sample prep kit v2 (Illumina) following the manufacturers protocol with 1µg total RNA input. 50 bp single end sequencing was performed using a HiSeq Illumina sequencer. Obtained sequences were de-multiplexed, quality controlled and mapped on the Aspergillus nidulans genome assembly (A_nidulans_FGSC_A4_version_s10-m03-r07).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>301855726</ID>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM1855726</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM1855727" accession="SRX1155639">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1155639</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM1855727</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM1855727: WT_A2; Fusarium graminearum PH-1; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP062498" refname="GSE72124">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP062498</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS1037823">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1037823</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM1855727</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>RNA isolation was performed using RNeasy Plant Mini Kit (Qiagen, 74904) following the instructions of the manufacturer with one modification and one extension: Instead of eluting RNA once in 30 µL RNase- free water, elution was performed in 2x 20 µL RNase- free water with a 3 minute standing period at room temperature after each water application to the column. Additionally on-column DNase digestion was done during the extraction protocol by using RNase-Free DNase Set (Qiagen, 79254). RNA quantity was first checked on the NanoDrop 2000c Spectrophotometer (Thermo Scientific). Afterwards RNA quantity and integrity was validated by using the Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer machine following the instructions of the provider. The cDNA Synthesis was done with the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, # K1632) using random hexamer primer and following the manufacturer´s protocol. Illumina sequencing libraries were made from RNA samples according to TruSeq RNA Sample prep kit v2 (Illumina) following the manufacturers protocol with 1µg total RNA input. 50 bp single end sequencing was performed using a HiSeq Illumina sequencer. Obtained sequences were de-multiplexed, quality controlled and mapped on the Aspergillus nidulans genome assembly (A_nidulans_FGSC_A4_version_s10-m03-r07).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>301855727</ID>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM1855727</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
</EXPERIMENT_SET>
