SRX1160347 GSM1858136 SRP062666 SRS1041373 GSM1858136 RNA-Seq TRANSCRIPTOMIC cDNA The gut tissue was dissected from ice-anesthetized third-instar larvae of Vip-Sel and Vip-Unsel subpopulations (25 larvae each) by cutting off the hindbody between the last two pairs of prolegs. Total RNA was extracted from gut samples (pools of five guts) using the total RNA isolation system (Promega, Madison, WI) according to manufacturer's instructions. RNA concentration was determined at 260 nm in a GeneQuant (Amersham Pharmacia, Amersham, UK) while the RNA quality was assessed by determining the 260 of 280 and 260 of 230 ratios. RNA pools of Vip-Sel and Vip-Unsel samples (five RNA preparations each) were prepared and used for the construction of SuperSAGE libraries HvR_GCCT and HvS_GCAC, respectively, according to the procedure described by Matsumura et al. [PLoS ONE (2010) vol 5: e12010). Illumina Genome Analyzer IIx gds 301858136 GEO Accession GSM1858136 SRX1160348 GSM1858137 SRP062666 SRS1041372 GSM1858137 RNA-Seq TRANSCRIPTOMIC cDNA The gut tissue was dissected from ice-anesthetized third-instar larvae of Vip-Sel and Vip-Unsel subpopulations (25 larvae each) by cutting off the hindbody between the last two pairs of prolegs. Total RNA was extracted from gut samples (pools of five guts) using the total RNA isolation system (Promega, Madison, WI) according to manufacturer's instructions. RNA concentration was determined at 260 nm in a GeneQuant (Amersham Pharmacia, Amersham, UK) while the RNA quality was assessed by determining the 260 of 280 and 260 of 230 ratios. RNA pools of Vip-Sel and Vip-Unsel samples (five RNA preparations each) were prepared and used for the construction of SuperSAGE libraries HvR_GCCT and HvS_GCAC, respectively, according to the procedure described by Matsumura et al. [PLoS ONE (2010) vol 5: e12010). Illumina Genome Analyzer IIx gds 301858137 GEO Accession GSM1858137