SRX1287950 Mixed Microbial Reference Sample SRP064204 1. Quantify Samples using Qubit. 2. Prepare 0.2 ng/uL dilutions of each 3. Follow Nextera XT protocol a. Tagmentation reaction following recommended procedure b. PCR indexing (N701 and N502, N503) c. PCR cleanup i. Two washes of Buffer PE ii. Two elutions of Buffer EB (20 uL each) d. Quantify using Qubit 4. Library Normalization a. Follow recommendations b. Qubit 5. Library pooling a. Use 12 uL of each normalized sample (approximately 13.4 pmol/L) b. Assume 500 bases as average size (did not measure) SRS1094331 SAMN04113077 MixedMicrobes WGS GENOMIC unspecified 600 0 Application Read Forward 1 1 Application Read Reverse 301 Illumina MiSeq SRX1291986 Mixed Microbial Reference Sample 1 SRP064204 1. Quantify Samples using Qubit. 2. Prepare 0.2 ng/uL dilutions of each 3. Follow Nextera XT protocol a. Tagmentation reaction following recommended procedure b. PCR indexing (N701 and N502, N503) c. PCR cleanup i. Two washes of Buffer PE ii. Two elutions of Buffer EB (20 uL each) d. Quantify using Qubit 4. Library Normalization a. Follow recommendations b. Qubit 5. Library pooling a. Use 12 uL of each normalized sample (approximately 13.4 pmol/L) b. Assume 500 bases as average size (did not measure) 6. Sequence using MiSeq SRS1094330 SAMN04113076 MixedMicrobial1 WGS GENOMIC unspecified 600 0 Application Read Forward 1 1 Application Read Reverse 301 Illumina MiSeq