SRS1099006 SAMN04145898 9606 Homo sapiens Male and female subjects aged 27-78 years (n =30) with a body mass index (BMI; in kg/m2) between 20 and 40 were selected on the basis of fasting serum total cholesterol (TC) between 5.0 and 8.0 mmol/L and LDL cholesterol (LDL-C) between 2.7 and 5.0 mmol/L. Potential volunteers were excluded using criteria including: intake of blood lipid lowering medications, herbal or nutritional supplements which are known to affect blood lipids and any dietary restrictions which would affect trial completion. No antibiotics intake was reported at the time of screening. A study physician evaluated the health history before the volunteers were accepted as subjects. The physician also provided medical supervision throughout the study. Written informed consent was obtained from all subjects. Of the 30 subjects in this study, a subset of 19 subjects who completed the entire protocol and provided fecal samples for all 4 study phases were selected for the microbiota profile analysis. The endpoint data for body weight, BMI, waist circumference, blood glucose and lipid profiles, and blood pressure for the 19 subjects included in the microbiome study extracted from previously reported data (Wang et al. J Nutr, underreview; Suppl. Table 1) and were reinterpreted in the context of their correlations with microbial changes evaluated in this research. isolate Manitoba Residents age 59 years biomaterial_provider Nancy Ames' Lab sex pooled male and female tissue Fecal samples BioSampleModel Human