Solid-state acetic acid fermentation Days-1 genome V4 region of 16S rRNA sequencing

SRX1315889 Fermentation Days-1 Solid-state acetic acid fermentation Days-1 genome V4 region of 16S rRNA sequencing SRP064586 PCR was carried out using genomic DNA as template targeting the V4 region of 16S/18S rRNA genes, PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina MiSeq platform using paired-end sequencing. SRS1107558 WGS METAGENOMIC PCR 506 0 Application Read Forward 1 1 Application Read Reverse 254 Illumina MiSeq SRX1315893 Fermentation Days-3 Solid-state acetic acid fermentation Days-3 genome V4 region of 16S rRNA sequencing SRP064586 PCR was carried out using genomic DNA as template targeting the V4 region of 16S/18S rRNA genes, PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina MiSeq platform using paired-end sequencing. SRS1107563 WGS METAGENOMIC PCR 506 0 Application Read Forward 1 1 Application Read Reverse 254 Illumina MiSeq SRX1315898 Fermentation Days-5 Solid-state acetic acid fermentation Days-5 genome V4 region of 16S rRNA sequencing SRP064586 PCR was carried out using genomic DNA as template targeting the V4 region of 16S/18S rRNA genes, PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina MiSeq platform using paired-end sequencing. SRS1107566 WGS METAGENOMIC PCR 506 0 Application Read Forward 1 1 Application Read Reverse 254 Illumina MiSeq SRX1315899 Fermentation Days-7 Solid-state acetic acid fermentation Days-7 genome V4 region of 16S rRNA sequencing SRP064586 PCR was carried out using genomic DNA as template targeting the V4 region of 16S/18S rRNA genes, PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina MiSeq platform using paired-end sequencing. SRS1107571 SAMN04155584 WGS METAGENOMIC PCR 506 0 Application Read Forward 1 1 Application Read Reverse 254 Illumina MiSeq SRX1316005 Fermentation Days-9 Solid-state acetic acid fermentation Days-9 genome V4 region of 16S rRNA sequencing SRP064586 PCR was carried out using genomic DNA as template targeting the V4 region of 16S/18S rRNA genes, PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina MiSeq platform using paired-end sequencing. SRS1107585 SAMN04155585 WGS METAGENOMIC PCR 506 0 Application Read Forward 1 1 Application Read Reverse 254 Illumina MiSeq SRX1316007 Fermentation Days-11 Solid-state acetic acid fermentation Days-11 genome V4 region of 16S rRNA sequencing SRP064586 PCR was carried out using genomic DNA as template targeting the V4 region of 16S/18S rRNA genes, PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina MiSeq platform using paired-end sequencing. SRS1107588 SAMN04155586 WGS METAGENOMIC PCR 506 0 Application Read Forward 1 1 Application Read Reverse 254 Illumina MiSeq SRX1316009 Fermentation Days-13 Solid-state acetic acid fermentation Days-13 genome V4 region of 16S rRNA sequencing SRP064586 PCR was carried out using genomic DNA as template targeting the V4 region of 16S/18S rRNA genes, PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina MiSeq platform using paired-end sequencing. SRS1107589 SAMN04155587 WGS METAGENOMIC PCR 506 0 Application Read Forward 1 1 Application Read Reverse 254 Illumina MiSeq SRX1316010 Fermentation Days-15 Solid-state acetic acid fermentation Days-15 genome V4 region of 16S rRNA sequencing SRP064586 PCR was carried out using genomic DNA as template targeting the V4 region of 16S/18S rRNA genes, PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina MiSeq platform using paired-end sequencing. SRS1107591 SAMN04155588 WGS METAGENOMIC PCR 506 0 Application Read Forward 1 1 Application Read Reverse 254 Illumina MiSeq SRX1316012 Fermentation Days-17 Solid-state acetic acid fermentation Days-17 genome V4 region of 16S rRNA sequencing SRP064586 PCR was carried out using genomic DNA as template targeting the V4 region of 16S/18S rRNA genes, PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina MiSeq platform using paired-end sequencing. SRS1107594 SAMN04155589 WGS METAGENOMIC PCR 506 0 Application Read Forward 1 1 Application Read Reverse 254 Illumina MiSeq SRX1316013 Fermentation Days-19 Solid-state acetic acid fermentation Days-19 genome V4 region of 16S rRNA sequencing SRP064586 PCR was carried out using genomic DNA as template targeting the V4 region of 16S/18S rRNA genes, PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina MiSeq platform using paired-end sequencing. SRS1107593 SAMN04155590 WGS METAGENOMIC PCR 506 0 Application Read Forward 1 1 Application Read Reverse 254 Illumina MiSeq