SRX1341826 GSM1910282 SRP064912 SRS1117385 GSM1910282 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910282 GEO Accession GSM1910282 SRX1341827 GSM1910283 SRP064912 SRS1117384 GSM1910283 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910283 GEO Accession GSM1910283 SRX1341828 GSM1910284 SRP064912 SRS1117383 GSM1910284 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910284 GEO Accession GSM1910284 SRX1341829 GSM1910285 SRP064912 SRS1117382 GSM1910285 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910285 GEO Accession GSM1910285 SRX1341830 GSM1910286 SRP064912 SRS1117381 GSM1910286 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910286 GEO Accession GSM1910286 SRX1341831 GSM1910287 SRP064912 SRS1117380 GSM1910287 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910287 GEO Accession GSM1910287 SRX1341832 GSM1910288 SRP064912 SRS1117379 GSM1910288 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910288 GEO Accession GSM1910288 SRX1341833 GSM1910289 SRP064912 SRS1117378 GSM1910289 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910289 GEO Accession GSM1910289 SRX1341834 GSM1910290 SRP064912 SRS1117377 GSM1910290 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910290 GEO Accession GSM1910290 SRX1341835 GSM1910291 SRP064912 SRS1117375 GSM1910291 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910291 GEO Accession GSM1910291 SRX1341836 GSM1910292 SRP064912 SRS1117374 GSM1910292 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910292 GEO Accession GSM1910292 SRX1341837 GSM1910293 SRP064912 SRS1117373 GSM1910293 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910293 GEO Accession GSM1910293 SRX1341838 GSM1910294 SRP064912 SRS1117372 GSM1910294 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910294 GEO Accession GSM1910294 SRX1341839 GSM1910295 SRP064912 SRS1117371 GSM1910295 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910295 GEO Accession GSM1910295 SRX1341840 GSM1910296 SRP064912 SRS1117376 GSM1910296 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910296 GEO Accession GSM1910296 SRX1341841 GSM1910297 SRP064912 SRS1117370 GSM1910297 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910297 GEO Accession GSM1910297 SRX1341842 GSM1910298 SRP064912 SRS1117369 GSM1910298 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910298 GEO Accession GSM1910298 SRX1341843 GSM1910299 SRP064912 SRS1117367 GSM1910299 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910299 GEO Accession GSM1910299 SRX1341844 GSM1910300 SRP064912 SRS1117368 GSM1910300 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910300 GEO Accession GSM1910300 SRX1341845 GSM1910301 SRP064912 SRS1117366 GSM1910301 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910301 GEO Accession GSM1910301 SRX1341846 GSM1910302 SRP064912 SRS1117365 GSM1910302 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910302 GEO Accession GSM1910302 SRX1341847 GSM1910303 SRP064912 SRS1117363 GSM1910303 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910303 GEO Accession GSM1910303 SRX1341848 GSM1910304 SRP064912 SRS1117364 GSM1910304 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910304 GEO Accession GSM1910304 SRX1341849 GSM1910305 SRP064912 SRS1117362 GSM1910305 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910305 GEO Accession GSM1910305 SRX1341850 GSM1910306 SRP064912 SRS1117361 GSM1910306 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910306 GEO Accession GSM1910306 SRX1341851 GSM1910307 SRP064912 SRS1117360 GSM1910307 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910307 GEO Accession GSM1910307 SRX1341852 GSM1910308 SRP064912 SRS1117359 GSM1910308 RNA-Seq TRANSCRIPTOMIC cDNA Regulatory T cells were sorted by FACS from single cell suspensions. Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample. Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen). cDNA quality was assessed using 2100 BioAnalyzer (Agilent). SOLiD protocols were followed for library prepartion for 50bp sequencing. AB SOLiD 4 System gds 301910308 GEO Accession GSM1910308