<?xml version="1.0" encoding="UTF-8"?>
<STUDY_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <STUDY center_name="GEO" alias="GSE74138" accession="SRP064968">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP064968</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject" label="primary">PRJNA299153</EXTERNAL_ID>
      <EXTERNAL_ID namespace="GEO">GSE74138</EXTERNAL_ID>
    </IDENTIFIERS>
    <DESCRIPTOR>
      <STUDY_TITLE>RNA-Seq analysis of 3 month old all Ret-positive (EGFP-labeled) dorsal root ganglia L345 or L6S1 neurons</STUDY_TITLE>
      <STUDY_TYPE existing_study_type="Transcriptome Analysis"/>
      <STUDY_ABSTRACT>The goal of this set is to build expression data using FACS isolated Ret-positive cells harvested from a Ret-EGFP reporter mouse (3 month old).  To enrich for bladder projecting neurons L6S1 DRGs were FACS isolated.  L3,4, 5 lumbar levels were also FACS isolated to aid in differential expression of Ret-positive populations from L6S1 and L345 and help enrich bladder Ret-positive neurons. The series further includes these populations from males and females to uncover sex based differences. Overall design: RNA was extracted from 3 month FACS isolated dorsal root ganglia dissected from Ret-EGFP C57BL/6J mice (EGFP knocked-in Ret locus-heterozyotes) and sequenced using Illumina Hiseq 2500 platform.</STUDY_ABSTRACT>
      <CENTER_PROJECT_NAME>GSE74138</CENTER_PROJECT_NAME>
    </DESCRIPTOR>
  </STUDY>
</STUDY_SET>
