SRX1360095 GSM1914920 SRP065234 SRS1124201 GSM1914920 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914920 GEO Accession GSM1914920 SRX1360096 GSM1914921 SRP065234 SRS1124199 GSM1914921 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914921 GEO Accession GSM1914921 SRX1360097 GSM1914922 SRP065234 SRS1124198 GSM1914922 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914922 GEO Accession GSM1914922 SRX1360098 GSM1914923 SRP065234 SRS1124197 GSM1914923 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914923 GEO Accession GSM1914923 SRX1360099 GSM1914924 SRP065234 SRS1124196 GSM1914924 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914924 GEO Accession GSM1914924 SRX1360100 GSM1914925 SRP065234 SRS1124193 GSM1914925 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914925 GEO Accession GSM1914925 SRX1360101 GSM1914926 SRP065234 SRS1124188 GSM1914926 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914926 GEO Accession GSM1914926 SRX1360102 GSM1914927 SRP065234 SRS1124187 GSM1914927 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914927 GEO Accession GSM1914927 SRX1360103 GSM1914928 SRP065234 SRS1124186 GSM1914928 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914928 GEO Accession GSM1914928 SRX1360104 GSM1914929 SRP065234 SRS1124185 GSM1914929 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914929 GEO Accession GSM1914929 SRX1360105 GSM1914930 SRP065234 SRS1124184 GSM1914930 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914930 GEO Accession GSM1914930 SRX1360106 GSM1914931 SRP065234 SRS1124183 GSM1914931 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914931 GEO Accession GSM1914931 SRX1360107 GSM1914932 SRP065234 SRS1124182 GSM1914932 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914932 GEO Accession GSM1914932 SRX1360108 GSM1914933 SRP065234 SRS1124181 GSM1914933 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914933 GEO Accession GSM1914933 SRX1360109 GSM1914934 SRP065234 SRS1124180 GSM1914934 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914934 GEO Accession GSM1914934 SRX1360110 GSM1914935 SRP065234 SRS1124179 GSM1914935 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914935 GEO Accession GSM1914935 SRX1360111 GSM1914936 SRP065234 SRS1124178 GSM1914936 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914936 GEO Accession GSM1914936 SRX1360112 GSM1914937 SRP065234 SRS1124177 GSM1914937 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914937 GEO Accession GSM1914937 SRX1360113 GSM1914938 SRP065234 SRS1124176 GSM1914938 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914938 GEO Accession GSM1914938 SRX1360114 GSM1914939 SRP065234 SRS1124175 GSM1914939 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914939 GEO Accession GSM1914939 SRX1360115 GSM1914940 SRP065234 SRS1124174 GSM1914940 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914940 GEO Accession GSM1914940 SRX1360116 GSM1914941 SRP065234 SRS1124173 GSM1914941 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914941 GEO Accession GSM1914941 SRX1360117 GSM1914942 SRP065234 SRS1124172 GSM1914942 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914942 GEO Accession GSM1914942 SRX1360118 GSM1914943 SRP065234 SRS1124171 GSM1914943 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914943 GEO Accession GSM1914943 SRX1360119 GSM1914944 SRP065234 SRS1124170 GSM1914944 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914944 GEO Accession GSM1914944 SRX1360120 GSM1914945 SRP065234 SRS1124169 GSM1914945 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914945 GEO Accession GSM1914945 SRX1360121 GSM1914946 SRP065234 SRS1124168 GSM1914946 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914946 GEO Accession GSM1914946 SRX1360122 GSM1914947 SRP065234 SRS1124167 GSM1914947 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914947 GEO Accession GSM1914947 SRX1360123 GSM1914948 SRP065234 SRS1124166 GSM1914948 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914948 GEO Accession GSM1914948 SRX1360124 GSM1914949 SRP065234 SRS1124165 GSM1914949 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914949 GEO Accession GSM1914949 SRX1360125 GSM1914950 SRP065234 SRS1124164 GSM1914950 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914950 GEO Accession GSM1914950 SRX1360126 GSM1914951 SRP065234 SRS1124163 GSM1914951 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914951 GEO Accession GSM1914951 SRX1360127 GSM1914952 SRP065234 SRS1124162 GSM1914952 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914952 GEO Accession GSM1914952 SRX1360128 GSM1914953 SRP065234 SRS1124161 GSM1914953 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914953 GEO Accession GSM1914953 SRX1360129 GSM1914954 SRP065234 SRS1124160 GSM1914954 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914954 GEO Accession GSM1914954 SRX1360130 GSM1914955 SRP065234 SRS1124159 GSM1914955 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914955 GEO Accession GSM1914955 SRX1360131 GSM1914956 SRP065234 SRS1124158 GSM1914956 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914956 GEO Accession GSM1914956 SRX1360132 GSM1914957 SRP065234 SRS1124157 GSM1914957 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914957 GEO Accession GSM1914957 SRX1360133 GSM1914958 SRP065234 SRS1124156 GSM1914958 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914958 GEO Accession GSM1914958 SRX1360134 GSM1914959 SRP065234 SRS1124155 GSM1914959 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914959 GEO Accession GSM1914959 SRX1360135 GSM1914960 SRP065234 SRS1124154 GSM1914960 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914960 GEO Accession GSM1914960 SRX1360136 GSM1914961 SRP065234 SRS1124153 GSM1914961 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914961 GEO Accession GSM1914961 SRX1360137 GSM1914962 SRP065234 SRS1124194 GSM1914962 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914962 GEO Accession GSM1914962 SRX1360138 GSM1914963 SRP065234 SRS1124195 GSM1914963 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914963 GEO Accession GSM1914963 SRX1360139 GSM1914964 SRP065234 SRS1124192 GSM1914964 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914964 GEO Accession GSM1914964 SRX1360140 GSM1914965 SRP065234 SRS1124191 GSM1914965 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914965 GEO Accession GSM1914965 SRX1360141 GSM1914966 SRP065234 SRS1124189 GSM1914966 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914966 GEO Accession GSM1914966 SRX1360142 GSM1914967 SRP065234 SRS1124190 GSM1914967 OTHER METAGENOMIC other Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing. Illumina MiSeq gds 301914967 GEO Accession GSM1914967