SRX1446232 Illumina Sequencing for Streptomyces eurocidicus ATCC 27428 SRP066567 PRJNA291301 Illumina PE TruSeq libraries were prepared as described in the manufactures methods (Part# 15005180 RevA) following the low throughput protocol. In short, 3 ug of DNA was sheared to a size between ~200 bp and 1000bp by nebulization (nitrogen) for 1 min at 30psi. The sheared DNA was purified on a Qiagen Qiaquick Spin column (Qiagen). The sheared material was accessed for quantity with a Qubit broad range double stranded DNA assay (Life Technologies) and quality by visualization on an Agilent Bioanalyzer DNA 7500 chip (Agilent). One microgram of sheared DNA was used for library preparation following the manufactures method. Steps included end repair followed by an AMPure XP bead clean up, adenylation of 3’ ends and adapter ligation followed by a second AMPure XP bead clean up, purification of the library by gel selection, DNA enrichment with 10 cycles of amplificaiton and a final AMPure XP bead clean up. Libraries were validated by Qubit (Life Technologies) and visualized by Agilent Bioanalyzer for appearance and size determination. Samples were normalized using the Illumina’s Library dilution calculator to a 10nM stock. Sequencing was completed on a Illumina MiSeq with a v2 500 cycle MiSeq kit sequencing 2 x 251 with 8bp for the index. SRS1173982 SAMN03941210 Streptomyces eurocidicus ATCC 27428 WGS GENOMIC RANDOM 502 0 Application Read Forward 1 1 Application Read Reverse 252 Illumina MiSeq SRX1446234 PacBio RS II for Streptomyces eurocidicus ATCC 27428 SRP066567 Pacific Biosciences single molecule, real-time DNA sequencing was performed by the University of Maryland School of Medicine Genomics Resource Center using P4-C2 chemistry SRS1173982 PacBio RS II for Streptomyces eurocidicus ATCC 27428 WGS GENOMIC RANDOM 0 0 Application Read Forward 1 PacBio RS II