SRX1492211 Mucus_metatranscriptome SRP067591 PRJNA305925 RNA was isolated using the MasterPure RNA purification kit and Dnase treated with Turbo DNA-free kit. mRNA was enriched using the Ribo-Zero Magnetic Kit (Gram-Negative Bacteria). 250 ng of the enriched mRNA was ethanol precipitated and eluted in Elute, Prime, Fragment mix. The eluted RNA was then processed using the TruSeq RNA Sample Prep Kit v2, and sequenced at 2 x 250 bp. SRS1213699 SAMN04335670 Mucus_metatranscriptome RNA-Seq METATRANSCRIPTOMIC RT-PCR Illumina MiSeq