SRX1522249 GSM2033155 SRP068231 SRS1240264 GSM2033155 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033155 GEO Accession GSM2033155 SRX1522250 GSM2033156 SRP068231 SRS1240263 GSM2033156 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033156 GEO Accession GSM2033156 SRX1522251 GSM2033157 SRP068231 SRS1240262 GSM2033157 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033157 GEO Accession GSM2033157 SRX1522252 GSM2033158 SRP068231 SRS1240261 GSM2033158 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033158 GEO Accession GSM2033158 SRX1522253 GSM2033159 SRP068231 SRS1240260 GSM2033159 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033159 GEO Accession GSM2033159 SRX1522254 GSM2033160 SRP068231 SRS1240259 GSM2033160 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033160 GEO Accession GSM2033160 SRX1522255 GSM2033161 SRP068231 SRS1240258 GSM2033161 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033161 GEO Accession GSM2033161 SRX1522256 GSM2033162 SRP068231 SRS1240257 GSM2033162 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033162 GEO Accession GSM2033162 SRX1522257 GSM2033163 SRP068231 SRS1240256 GSM2033163 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033163 GEO Accession GSM2033163 SRX1522258 GSM2033164 SRP068231 SRS1240255 GSM2033164 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033164 GEO Accession GSM2033164 SRX1522259 GSM2033165 SRP068231 SRS1240254 GSM2033165 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033165 GEO Accession GSM2033165 SRX1522260 GSM2033166 SRP068231 SRS1240253 GSM2033166 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033166 GEO Accession GSM2033166 SRX1522261 GSM2033168 SRP068231 SRS1240251 GSM2033168 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033168 GEO Accession GSM2033168 SRX1522262 GSM2033169 SRP068231 SRS1240252 GSM2033169 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033169 GEO Accession GSM2033169 SRX1522263 GSM2033170 SRP068231 SRS1240250 GSM2033170 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033170 GEO Accession GSM2033170 SRX1522264 GSM2033171 SRP068231 SRS1240249 GSM2033171 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033171 GEO Accession GSM2033171 SRX1522265 GSM2033172 SRP068231 SRS1240248 GSM2033172 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033172 GEO Accession GSM2033172 SRX1522266 GSM2033173 SRP068231 SRS1240247 GSM2033173 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033173 GEO Accession GSM2033173 SRX1522267 GSM2033174 SRP068231 SRS1240246 GSM2033174 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033174 GEO Accession GSM2033174 SRX1522268 GSM2033175 SRP068231 SRS1240245 GSM2033175 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033175 GEO Accession GSM2033175 SRX1522269 GSM2033176 SRP068231 SRS1240244 GSM2033176 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033176 GEO Accession GSM2033176 SRX1522270 GSM2033177 SRP068231 SRS1240243 GSM2033177 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033177 GEO Accession GSM2033177 SRX1522271 GSM2033178 SRP068231 SRS1240242 GSM2033178 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033178 GEO Accession GSM2033178 SRX1553159 GSM2033167 SRP068231 SRS1269069 SAMN04390646 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Total RNA was treated with RQ1 DNase (Promega, Madison, Wisconsin, USA) to remove DNA. For each sample, 10 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, California, USA) before use for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95 °C, followed by end repair and 5′ adaptor ligation. Reverse transcription was performed with the RT primers harboring the 3’ adaptor sequence and randomized hexamers. The cDNAs were purified and amplified. The PCR products corresponding to 200–500 bp were purified, quantified and then stored at –80 °C until used for sequencing. Total RNA (3 μg) was used for sRNA cDNA library preparation with the Balancer NGS Library Preparation Kit for sRNA (GnomeGen, San Diego, CA, USA) in accordance with the manufacture's instructions. In brief, RNAs were sequentially ligated to 3′- and 5′-end adaptors, reverse transcribed to cDNA and then amplified by PCR. Whole libraries were applied to 10% native PAGE gel electrophoresis; bands corresponding to mRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified sRNA libraries were quantified with a Qubit Fluorometer (Invitrogen, Carlsbad, California, USA) before cluster generation and 36 nt single-end sequencing analysis using the Illumina GAIIx (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions. Illumina Genome Analyzer IIx gds 302033167 GEO Accession GSM2033167