SRX1528956 siol bacteria SRP068437 PRJNA308766 The field experiment was established in 2011 to investigate the effect on soil microbial communities of continuous application of farmyard manure, crop residues, and mineral fertilizer (NPK) in a winter wheat-summer maize rotation. Micro plot trial for maize (Zea mays L.) was carried out at the farm of Linquan Agriculture Research Institute (32°55′N/115°06′E), Anhui Province, China as a randomized complete block design with 17 treatments replicated three times (three blocks). These treatments are W1 (nofertilizer), W2 (100% NPK), W3 (pig manure 200kg and 70% NPK), W4 (cattle manure 200kg and 70% NPK), W5 (pig manure 400kg and 50% NPK), W6 (cattle manure 400kg and 50% NPK), W7 (return of wheat straw and 100% NPK), W8 (return of wheat straw, biological decomposing agents 10kg, and 100% NPK), W9 (return of wheat straw, biological decomposing agents 20kg, and 100% NPK), W10 (pig manure 200kg, return of wheat straw, and 70% NPK), W11 (cattle manure 200kg, return of wheat straw, and 70% NPK), W12 (pig manure 400kg, return of wheat straw, and 50% NPK), W13 (cattle manure 400kg, return of wheat straw, and 50% NPK), W14 (inorganic and organic pig manure admixture fertilizer 120 kg), W15 (inorganic and organic cattle manure admixture fertilizer 120 kg), W16 (inorganic and organic pig manure admixture fertilizer 240 kg), and W17 (inorganic and organic cattle manure admixture fertilizer 240 kg), respectively. Each plot is a rectangle with an area of 50 m2 (5×10 m2). The averages of the soils characteristics are as follows: pH 5.88, organic matter (12.64 g•Kg−1), total N (1.21 g•Kg−1), total P (0.36 g•Kg−1), and available P (16.92 mg of P kg−1). Soil samples for isolating DNA were collected at the summer maize harvest time in 2014. SRS1247834 SAMN04393623 OTHER OTHER PCR 1000 0 Application Read Forward 1 1 Application Read Reverse 501 Illumina MiSeq