<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT alias="GSM2036558" accession="SRX1527116">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1527116</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM2036558</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM2036558: Muscle.Treg.IL33; Mus musculus; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP068306" refname="GSE76722">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP068306</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS1244923">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1244923</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM2036558</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>RNA was extracted from Trizol using chloroform. RNA was purified using RNA-SPRI beads. Poly(A)-mRNA was converted to cDNA and then amplified. cDNA was subject to transposon-based fragmentation  that used dual-indexing to barcode each fragment of each converted transcript with a combination of barcodes specific to each sample. Sequencing was carried out as paired end 2x25bp with an additional 8 cycles for each index.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>302036558</ID>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM2036558</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM2036559" accession="SRX1527117">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1527117</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM2036559</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM2036559: Muscle.Treg.PBS; Mus musculus; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP068306" refname="GSE76722">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP068306</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS1244922">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1244922</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM2036559</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>RNA was extracted from Trizol using chloroform. RNA was purified using RNA-SPRI beads. Poly(A)-mRNA was converted to cDNA and then amplified. cDNA was subject to transposon-based fragmentation  that used dual-indexing to barcode each fragment of each converted transcript with a combination of barcodes specific to each sample. Sequencing was carried out as paired end 2x25bp with an additional 8 cycles for each index.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>302036559</ID>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM2036559</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
</EXPERIMENT_SET>
