SRX1527803 IJOWM9E_region1 SRP068327 david_shen_lpd Mice were fed a low protein diet or normal and gavaged with normal flora or ASF SRS1245615 IJOWM9E_region1_default SRS1245616 BLS031639_LPD1.baseline barcode primer SRS1245617 BLS031640_LPD2.baseline barcode primer SRS1245618 BLS031641_LPD3.baseline barcode primer SRS1245619 BLS031642_LPD4.baseline barcode primer SRS1245620 BLS031643_LPD5.baseline barcode primer SRS1245621 BLS031644_LPD6.baseline barcode primer SRS1245622 BLS031645_LPD7.baseline barcode primer SRS1245623 BLS031646_LPD8.baseline barcode primer SRS1245624 BLS031647_LPD9.baseline barcode primer SRS1245625 BLS031648_LPD10.baseline barcode primer SRS1245626 BLS031649_LPD1.beforeFMT.2wk barcode primer SRS1245627 BLS031650_LPD2.beforeFMT.2wk barcode primer SRS1245628 BLS031651_LPD3.beforeFMT.2wk barcode primer SRS1245629 BLS031652_LPD4.beforeFMT.2wk barcode primer SRS1245630 BLS031653_LPD5.beforeFMT.2wk barcode primer SRS1245631 BLS031654_LPD6.beforeFMT.2wk barcode primer SRS1245632 BLS031655_LPD7.beforeFMT.2wk barcode primer SRS1245633 BLS031656_LPD8.beforeFMT.2wk barcode primer SRS1245634 BLS031657_LPD9.beforeFMT.2wk barcode primer SRS1245635 BLS031658_LPD10.beforeFMT.2wk barcode primer SRS1245636 BLS031659_LPD1.postFMT.2wk barcode primer SRS1245637 BLS031660_LPD2.postFMT.2wk barcode primer SRS1245638 BLS031661_LPD3.postFMT.2wk barcode primer SRS1245639 BLS031662_LPD4.postFMT.2wk barcode primer SRS1245640 BLS031663_LPD5.postFMT.2wk barcode primer SRS1245641 BLS031664_LPD6.postFMT.2wk barcode primer SRS1245642 BLS031665_LPD7.postFMT.2wk barcode primer SRS1245643 BLS031666_LPD8.postFMT.2wk barcode primer SRS1245644 BLS031667_LPD9.postFMT.2wk barcode primer SRS1245645 BLS031668_LPD10.postFMT.2wk barcode primer SRS1245646 BLS031669_LPD1.postFMT.1mo barcode primer SRS1245647 BLS031670_LPD2.postFMT.1mo barcode primer SRS1245648 BLS031671_LPD3.postFMT.1mo barcode primer SRS1245649 BLS031672_LPD4.postFMT.1mo barcode primer SRS1245650 BLS031673_LPD5.postFMT.1mo barcode primer SRS1245651 BLS031674_LPD6.postFMT.1mo barcode primer SRS1245652 BLS031675_LPD8.postFMT.1mo barcode primer SRS1245653 BLS031676_LPD9.postFMT.1mo barcode primer SRS1245654 BLS031677_LPD10.postFMT.1mo barcode primer SRS1245655 BLS031678_LPD1.postFMT.2.5mo barcode primer SRS1245656 BLS031679_LPD2.postFMT.2.5mo barcode primer SRS1245657 BLS031680_LPD3.postFMT.2.5mo barcode primer SRS1245658 BLS031681_LPD4.postFMT.2.5mo barcode primer SRS1245659 BLS031682_LPD5.postFMT.2.5mo barcode primer SRS1245660 BLS031683_LPD6.postFMT.2.5mo barcode primer SRS1245661 BLS031684_LPD8.postFMT.2.5mo barcode primer SRS1245662 BLS031685_LPD9.postFMT.2.5mo barcode primer SRS1245663 BLS031686_LPD10.postFMT.2.5mo barcode primer IJOWM9E_region1 AMPLICON METAGENOMIC PCR Each amplicon library was constructed by amplifying the16S rRNA gene using the BSF8 and and the BSR357 primer pair. Primers contained DNA barcode sequences such as those described by Hamady et al. 2008, and the recommended 454 adapter sequences. Amplification conditions are described in McKenna et al 2007, with exception that the polymerase used was AccuPrime (Invitrogen, Carlsbad, CA, USA). 0 Technical Read Adapter TCAG 1 barcode Technical Read BarCode CATCAGGT CCAAGGTT CCATGGAT CCGGAATT CCGGATAT CCGGTAAT TTCGAAGC CCTAGGAT CGAACGTT CGAAGCTT TGCTCAAG TTAACCGG TTAACGCG TTAAGCCG TTCCAAGG TTCGAACG TTGCAAGC TTGCAACG AACGGCTT AAGCCGTT ACAGGTCT ACTGGACT AGACCTGT AGTCCAGT ATCGGCAT ATGCCGAT TTGGAACC CAACTGGT CAAGTCGT CAAGTGCT CGCGAATT CGCGTAAT CGGCAATT CGGCATAT CGGCTAAT CTACAGGT GAAGTCCT GATGACCT GCCGAATT TATAGGCC TCGTGAAC TGCTGAAC TGGACATC TGGACTAC TGGTCAAC TTAACGGC TTAAGCGC TTAAGGCC 2 primer Technical Read Primer CTGCTGCCTYCCGTA 3 Application Read Forward 454 GS Junior