SRX1736086 GSM2137056 SRP074147 PRJNA319983 SRS1416933 SAMN04914062 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137056 GSM2137056 GEO Accession GSM2137056 SRX1736087 GSM2137057 SRP074147 PRJNA319983 SRS1416934 SAMN04914063 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137057 GSM2137057 GEO Accession GSM2137057 SRX1736088 GSM2137058 SRP074147 PRJNA319983 SRS1416935 SAMN04914064 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137058 GSM2137058 GEO Accession GSM2137058 SRX1736089 GSM2137059 SRP074147 PRJNA319983 SRS1416936 SAMN04914065 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137059 GSM2137059 GEO Accession GSM2137059 SRX1736090 GSM2137060 SRP074147 PRJNA319983 SRS1416937 SAMN04914066 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137060 GSM2137060 GEO Accession GSM2137060 SRX1736091 GSM2137061 SRP074147 PRJNA319983 SRS1416938 SAMN04914067 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137061 GSM2137061 GEO Accession GSM2137061 SRX1736092 GSM2137062 SRP074147 PRJNA319983 SRS1416939 SAMN04914068 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137062 GSM2137062 GEO Accession GSM2137062 SRX1736093 GSM2137063 SRP074147 PRJNA319983 SRS1416940 SAMN04914069 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137063 GSM2137063 GEO Accession GSM2137063 SRX1736094 GSM2137064 SRP074147 PRJNA319983 SRS1416941 SAMN04914070 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137064 GSM2137064 GEO Accession GSM2137064 SRX1736095 GSM2137065 SRP074147 PRJNA319983 SRS1416942 SAMN04914071 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137065 GSM2137065 GEO Accession GSM2137065 SRX1736096 GSM2137066 SRP074147 PRJNA319983 SRS1416943 SAMN04914072 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137066 GSM2137066 GEO Accession GSM2137066 SRX1736097 GSM2137067 SRP074147 PRJNA319983 SRS1416944 SAMN04914073 RNA-Seq TRANSCRIPTOMIC cDNA For cell lysis, the FastRNA Pro Blue kit (MP Bio) was used whilst RNA was isolated and cleaned up using the Total RNA Purification Plus kit (Norgen). Cell pellets were resuspended in 1ml of the phenol based lysis solution and added to the supplied bead matrix. The samples were disrupted twice for 20 seconds using the Precellys 24 homogeniser (Bertin Technologies). After centrifugation, the supernatant (750 μl) was added to 300 μl of chloroform. After a 5 minute incubation at room temperature, the samples were centrifuged at full speed at 4 ᵒC. The resultant aqueous phase was added to an equal volume of the Norgen lysis buffer and the RNA was purified as per the exact instructions supplied with the Total RNA Purification Plus kit. The quality and concentration of the total RNA was determined an Agilent Bioanalyser 2100 using the RNA 6000 Nano kit. Sequencing was performed by Vertis Biotechnologie AG (Germany). From the total RNA samples, ribosomal RNA molecules were depleted using Ribo-Zero rRNA Removal Kit for bacteria (Epicentre). RNA samples were fragmented using RNaseIII. The samples were then poly(A)-tailed using poly(A) polymerase. The samples were then treated with RNA 5' Polyphosphatase (+5'PP, Epicentre) in order to convert 5'PPP structures into 5' monophosphate ends. Afterwards, an RNA adapter was ligated to the 5'-monophosphate of the RNA. First- strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a high fidelity DNA polymerase. NextSeq 500 gds 302137067 GSM2137067 GEO Accession GSM2137067