SRX1761415 s45t1_12 <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437138"> <IDENTIFIERS> <PRIMARY_ID>SRS1437138</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999935</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s45t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761416" alias="s19t1_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761416</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s19t1_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437139"> <IDENTIFIERS> <PRIMARY_ID>SRS1437139</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999936</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s19t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761417" alias="s40t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761417</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s40t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437140"> <IDENTIFIERS> <PRIMARY_ID>SRS1437140</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999945</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s40t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761418" alias="s27t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761418</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s27t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437141"> <IDENTIFIERS> <PRIMARY_ID>SRS1437141</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999946</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s27t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761419" alias="s28t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761419</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s28t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437142"> <IDENTIFIERS> <PRIMARY_ID>SRS1437142</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999947</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s28t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761420" alias="s45t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761420</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s45t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437143"> <IDENTIFIERS> <PRIMARY_ID>SRS1437143</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999948</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s45t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761421" alias="s19t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761421</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s19t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437144"> <IDENTIFIERS> <PRIMARY_ID>SRS1437144</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999949</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s19t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761422" alias="s24t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761422</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s24t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437145"> <IDENTIFIERS> <PRIMARY_ID>SRS1437145</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999950</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s24t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761423" alias="s41t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761423</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s41t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437146"> <IDENTIFIERS> <PRIMARY_ID>SRS1437146</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999951</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s41t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761424" alias="s20t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761424</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s20t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437147"> <IDENTIFIERS> <PRIMARY_ID>SRS1437147</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999952</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s20t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761425" alias="s27t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761425</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s27t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437149"> <IDENTIFIERS> <PRIMARY_ID>SRS1437149</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999953</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s27t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761426" alias="s44t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761426</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s44t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437148"> <IDENTIFIERS> <PRIMARY_ID>SRS1437148</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999954</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s44t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761427" alias="s46t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761427</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s46t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437150"> <IDENTIFIERS> <PRIMARY_ID>SRS1437150</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999937</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s46t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761428" alias="s20t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761428</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s20t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437151"> <IDENTIFIERS> <PRIMARY_ID>SRS1437151</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999955</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s20t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761429" alias="s28t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761429</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s28t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437152"> <IDENTIFIERS> <PRIMARY_ID>SRS1437152</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999956</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s28t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761430" alias="s44t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761430</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s44t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437153"> <IDENTIFIERS> <PRIMARY_ID>SRS1437153</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999957</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s44t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761431" alias="s40t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761431</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s40t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437155"> <IDENTIFIERS> <PRIMARY_ID>SRS1437155</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999958</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s40t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761432" alias="s45t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761432</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s45t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437154"> <IDENTIFIERS> <PRIMARY_ID>SRS1437154</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999959</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s45t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761433" alias="s19t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761433</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s19t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437156"> <IDENTIFIERS> <PRIMARY_ID>SRS1437156</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999960</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s19t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761434" alias="s46t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761434</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s46t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437157"> <IDENTIFIERS> <PRIMARY_ID>SRS1437157</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999961</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s46t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761435" alias="s36t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761435</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s36t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437158"> <IDENTIFIERS> <PRIMARY_ID>SRS1437158</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999962</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s36t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761436" alias="s36t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761436</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s36t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437159"> <IDENTIFIERS> <PRIMARY_ID>SRS1437159</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999963</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s36t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761437" alias="s37t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761437</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s37t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437160"> <IDENTIFIERS> <PRIMARY_ID>SRS1437160</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999964</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s37t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761438" alias="s36t1_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761438</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s36t1_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437161"> <IDENTIFIERS> <PRIMARY_ID>SRS1437161</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999938</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s36t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761439" alias="s37t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761439</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s37t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437162"> <IDENTIFIERS> <PRIMARY_ID>SRS1437162</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999965</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s37t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761440" alias="s24t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761440</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s24t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437163"> <IDENTIFIERS> <PRIMARY_ID>SRS1437163</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999966</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s24t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761441" alias="s46t1_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761441</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s46t1_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437164"> <IDENTIFIERS> <PRIMARY_ID>SRS1437164</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999967</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s46t1_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761442" alias="s41t2_13"> <IDENTIFIERS> <PRIMARY_ID>SRX1761442</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s41t2_13</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437165"> <IDENTIFIERS> <PRIMARY_ID>SRS1437165</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999968</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s41t2_13</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761443" alias="oli1"> <IDENTIFIERS> <PRIMARY_ID>SRX1761443</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">oli1</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level 20-30 cm from peat surface. Peat was stored at -80 degrees Celsius. RNA was extracted using a phenol-chloroform method. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437166"> <IDENTIFIERS> <PRIMARY_ID>SRS1437166</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999969</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>oli1</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761444" alias="oli2"> <IDENTIFIERS> <PRIMARY_ID>SRX1761444</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">oli2</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level 20-30 cm from peat surface. Peat was stored at -80 degrees Celsius. RNA was extracted using a phenol-chloroform method. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437167"> <IDENTIFIERS> <PRIMARY_ID>SRS1437167</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999970</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>oli2</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761445" alias="oli3"> <IDENTIFIERS> <PRIMARY_ID>SRX1761445</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">oli3</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level 20-30 cm from peat surface. Peat was stored at -80 degrees Celsius. RNA was extracted using a phenol-chloroform method. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437168"> <IDENTIFIERS> <PRIMARY_ID>SRS1437168</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999971</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>oli3</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761446" alias="mes2"> <IDENTIFIERS> <PRIMARY_ID>SRX1761446</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">mes2</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level 20-30 cm from peat surface. Peat was stored at -80 degrees Celsius. RNA was extracted using a phenol-chloroform method. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437169"> <IDENTIFIERS> <PRIMARY_ID>SRS1437169</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999972</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>mes2</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761447" alias="mes3"> <IDENTIFIERS> <PRIMARY_ID>SRX1761447</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">mes3</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level 20-30 cm from peat surface. Peat was stored at -80 degrees Celsius. RNA was extracted using a phenol-chloroform method. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437170"> <IDENTIFIERS> <PRIMARY_ID>SRS1437170</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999973</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>mes3</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761448" alias="mes4"> <IDENTIFIERS> <PRIMARY_ID>SRX1761448</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">mes4</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level 20-30 cm from peat surface. Peat was stored at -80 degrees Celsius. RNA was extracted using a phenol-chloroform method. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437171"> <IDENTIFIERS> <PRIMARY_ID>SRS1437171</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999974</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>mes4</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761449" alias="s36t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761449</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s36t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437172"> <IDENTIFIERS> <PRIMARY_ID>SRS1437172</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999939</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s36t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761450" alias="s40t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761450</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s40t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437173"> <IDENTIFIERS> <PRIMARY_ID>SRS1437173</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999975</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s40t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761451" alias="s27t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761451</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s27t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437176"> <IDENTIFIERS> <PRIMARY_ID>SRS1437176</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999976</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s27t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761452" alias="s28t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761452</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s28t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437175"> <IDENTIFIERS> <PRIMARY_ID>SRS1437175</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999977</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s28t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761453" alias="s45t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761453</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s45t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437174"> <IDENTIFIERS> <PRIMARY_ID>SRS1437174</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999978</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s45t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761454" alias="s19t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761454</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s19t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437177"> <IDENTIFIERS> <PRIMARY_ID>SRS1437177</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999979</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s19t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761455" alias="s24t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761455</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s24t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437178"> <IDENTIFIERS> <PRIMARY_ID>SRS1437178</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999980</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s24t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761456" alias="s41t1_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761456</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s41t1_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437179"> <IDENTIFIERS> <PRIMARY_ID>SRS1437179</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999981</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s41t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761457" alias="s20t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761457</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s20t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437180"> <IDENTIFIERS> <PRIMARY_ID>SRS1437180</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999982</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s20t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761458" alias="s27t1_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761458</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s27t1_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437181"> <IDENTIFIERS> <PRIMARY_ID>SRS1437181</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999983</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s27t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761459" alias="s44t1_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761459</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s44t1_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437182"> <IDENTIFIERS> <PRIMARY_ID>SRS1437182</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999984</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s44t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761460" alias="s37t1_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761460</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s37t1_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437183"> <IDENTIFIERS> <PRIMARY_ID>SRS1437183</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999940</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s37t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761461" alias="s20t1_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761461</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s20t1_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437184"> <IDENTIFIERS> <PRIMARY_ID>SRS1437184</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999985</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s20t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761462" alias="s28t1_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761462</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s28t1_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437185"> <IDENTIFIERS> <PRIMARY_ID>SRS1437185</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999986</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s28t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761463" alias="s44t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761463</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s44t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437186"> <IDENTIFIERS> <PRIMARY_ID>SRS1437186</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999987</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s44t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761464" alias="s40t1_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761464</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s40t1_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437187"> <IDENTIFIERS> <PRIMARY_ID>SRS1437187</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999988</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s40t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761465" alias="s37t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761465</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s37t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437188"> <IDENTIFIERS> <PRIMARY_ID>SRS1437188</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999941</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s37t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761466" alias="s24t1_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761466</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s24t1_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437189"> <IDENTIFIERS> <PRIMARY_ID>SRS1437189</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999942</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s24t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761467" alias="s46t1_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761467</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s46t1_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437190"> <IDENTIFIERS> <PRIMARY_ID>SRS1437190</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999943</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s46t1_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> <EXPERIMENT accession="SRX1761468" alias="s41t2_12"> <IDENTIFIERS> <PRIMARY_ID>SRX1761468</PRIMARY_ID> <SUBMITTER_ID namespace="SUB1513872">s41t2_12</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="SRP075161"> <IDENTIFIERS> <PRIMARY_ID>SRP075161</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA321486</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Peat cores were collected from below the water level at 20-30 cm from peat surface. Peat was used in a stable isotope probing experiment with 13C-cellobiose under anoxic conditions. RNA was extracted using a phenol-chloroform method and separated into heavy (13C) and light (12C) fractions with isopycnic centrifugation in a cesium trifluoroacetate. After reverse transcription, 16S rRNA genes were amplified with bacterial primers 341F and 804R (Herrleman et al. 2011).</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1437191"> <IDENTIFIERS> <PRIMARY_ID>SRS1437191</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN04999944</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>s41t2_12</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> </EXPERIMENT> </EXPERIMENT_SET>