SRX1761864
m11_1_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437470
SAMN05000783
m11_1_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761865
m11_2_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437471
SAMN05000784
m11_2_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761866
m11_11_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437473
SAMN05000793
m11_11_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761867
m11_12_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437472
SAMN05000794
m11_12_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761868
m10_1_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437474
SAMN05000795
m10_1_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761869
m10_2_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437475
SAMN05000796
m10_2_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761870
m10_3_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437476
SAMN05000797
m10_3_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761871
m10_4_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437478
SAMN05000798
m10_4_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761872
m10_5_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437477
SAMN05000799
m10_5_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761873
m10_6_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437479
SAMN05000800
m10_6_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761874
m10_7_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437480
SAMN05000801
m10_7_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761875
m10_8_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437481
SAMN05000802
m10_8_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761876
m11_3_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437482
SAMN05000785
m11_3_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761877
m10_9_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437483
SAMN05000803
m10_9_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761878
m10_10_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437484
SAMN05000804
m10_10_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761879
m10_11_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437485
SAMN05000805
m10_11_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761880
m10_12_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437486
SAMN05000806
m10_12_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761881
ino1_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437487
SAMN05000807
ino1_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761882
ino2_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437488
SAMN05000808
ino2_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761883
m11_4_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437489
SAMN05000786
m11_4_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761884
m11_5_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437490
SAMN05000787
m11_5_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761885
m11_6_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437491
SAMN05000788
m11_6_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761886
m11_7_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437492
SAMN05000789
m11_7_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761887
m11_8_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437493
SAMN05000790
m11_8_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761888
m11_9_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437494
SAMN05000791
m11_9_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq
SRX1761889
m11_10_1
SRP075169
PRJNA321479
Water samples were collected from continuous reactors prior and post one month treatments which consisted of feeding the reactors either with artificial lake water media and continuous acetate or with pulses of acetate, each treatment had in total 6 replicates. A total of 10 mL of water was collected from each reactor by vacuum filtration through 0.2 µm Supor 200 filters (Pall Corporation, Port Washington, NY, USA). Filters were stored at – 80 ̊C until DNA extraction was performed using the Power Soil DNA isolation kit as recommended by the manufacturer (MoBio Laboratories, Carlsbad, CA, USA). Each sample was initially amplified in duplicates using bacterial primer constructs targeting the variable V4 region of the 16S rRNA gene. Duplicates were pooled and purified using the Agencourt AMPure (Beckman Coulter) as recommended by the manufacturer, and then used as template in a second PCR step in order to attach standard Illumina handles and index/barcode primers N4-341F and N4-805R. PCR products were purified in the same fashion.
SRS1437495
SAMN05000792
m11_10_1
AMPLICON
METAGENOMIC
PCR
Illumina MiSeq