SRX1770904 RedSea_StA_Summer_surface SRP075317 PRJNA321245 RNA extraction, Dnase treatment, rRNA depletion (RiboZero - bacteria kit), purification, tRNA removal (Agencourt RNAClean XP kit - Beckman Coulter genomics), sonication, purification, first strand cDNA synthesis (N6 randomized primers), adapter ligation at both ends (3' adapter included barcodes), PCR amplification (17 cycles with high fidelity polimerase), cDNA purification, equimolar pooling of barcoded samples, size selection (300-600bp) using preparative gel SRS1443219 SAMN04978334 RedSea_StA_Summer_surface RNA-Seq METATRANSCRIPTOMIC RANDOM PCR 150 0 Application Read Forward 1 1 Application Read Reverse 76 Illumina HiSeq 2000 SRX1770905 RedSea_StA_Summer_DCM SRP075317 PRJNA321245 RNA extraction, Dnase treatment, rRNA depletion (RiboZero - bacteria kit), purification, tRNA removal (Agencourt RNAClean XP kit - Beckman Coulter genomics), sonication, purification, first strand cDNA synthesis (N6 randomized primers), adapter ligation at both ends (3' adapter included barcodes), PCR amplification (17 cycles with high fidelity polimerase), cDNA purification, equimolar pooling of barcoded samples, size selection (300-600bp) using preparative gel SRS1443220 SAMN04978335 RedSea_StA_Summer_DCM RNA-Seq METATRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 2000 SRX1770906 RedSea_StA_Summer_aphotic SRP075317 PRJNA321245 RNA extraction, Dnase treatment, rRNA depletion (RiboZero - bacteria kit), purification, tRNA removal (Agencourt RNAClean XP kit - Beckman Coulter genomics), sonication, purification, first strand cDNA synthesis (N6 randomized primers), adapter ligation at both ends (3' adapter included barcodes), PCR amplification (17 cycles with high fidelity polimerase), cDNA purification, equimolar pooling of barcoded samples, size selection (300-600bp) using preparative gel SRS1443221 SAMN04978336 RedSea_StA_Summer_aphotic RNA-Seq METATRANSCRIPTOMIC RANDOM PCR 150 0 Application Read Forward 1 1 Application Read Reverse 76 Illumina HiSeq 2000