SRX1840926 GSM2199317 SRP076488 SRS1500107 GSM2199317 RNA-Seq TRANSCRIPTOMIC cDNA Sorted P6 ID4-EGFP+ spermatogonia and subpopulations defined by antibody labeling for TSPAN8, EPHA2, or PVR were used for RNA-seq analysis of transcriptomes. For this purpose, sorted cells were pelleted, counted and subjected to direct cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, Mountain View, CA) per manufacturer recommendations and 9 cycles of amplification. Amplified cDNAs from each sample were purified using Agencourt AMPure XP Pure magnetic beads (Beckman Coulter Life Sciences, Indianapolis, IN) and pre-qualified for library construction using a 2100 Bioanalyzer and High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA). Using 250pg input cDNA, we performed Nextera XT dual-index library preparation with modifications from manufacturer recommendations. Specifically, tagmentation was performed with 2.5ul Tagment DNA buffer, 1.25ul Amplification Tagment Mix, and 1.25ul cDNA for exactly 10 minutes at 55°C, ramp to 10°C, and immediate addition of 1.25ul NT buffer. Additionally, PCR amplification with index primers (separate index per sample) was performed with the entire 6.25ul of Tagmentation reaction mix plus 3.75ul Nextera PCR Mix using the recommended cycling conditions except that the extension time was 60 seconds. Libraries were quantified with Qubit fluorometry (ThermoFisher Scientific) and qualified for fragment size and distribution by 2100 Bioanalyzer with High-sensitivity DNA reagents (522 ± 6 bp). Libraries were pooled at equal concentrations and subjected to four lanes of rapid mode Illumina HiSeq2500 sequencing (paired-end 100bp) at the University of Texas Southwestern Medical Center Genomics and Microarray Core. Illumina HiSeq 2500 gds 302199317 GEO Accession GSM2199317 SRX1840927 GSM2199318 SRP076488 SRS1500108 GSM2199318 RNA-Seq TRANSCRIPTOMIC cDNA Sorted P6 ID4-EGFP+ spermatogonia and subpopulations defined by antibody labeling for TSPAN8, EPHA2, or PVR were used for RNA-seq analysis of transcriptomes. For this purpose, sorted cells were pelleted, counted and subjected to direct cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, Mountain View, CA) per manufacturer recommendations and 9 cycles of amplification. Amplified cDNAs from each sample were purified using Agencourt AMPure XP Pure magnetic beads (Beckman Coulter Life Sciences, Indianapolis, IN) and pre-qualified for library construction using a 2100 Bioanalyzer and High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA). Using 250pg input cDNA, we performed Nextera XT dual-index library preparation with modifications from manufacturer recommendations. Specifically, tagmentation was performed with 2.5ul Tagment DNA buffer, 1.25ul Amplification Tagment Mix, and 1.25ul cDNA for exactly 10 minutes at 55°C, ramp to 10°C, and immediate addition of 1.25ul NT buffer. Additionally, PCR amplification with index primers (separate index per sample) was performed with the entire 6.25ul of Tagmentation reaction mix plus 3.75ul Nextera PCR Mix using the recommended cycling conditions except that the extension time was 60 seconds. Libraries were quantified with Qubit fluorometry (ThermoFisher Scientific) and qualified for fragment size and distribution by 2100 Bioanalyzer with High-sensitivity DNA reagents (522 ± 6 bp). Libraries were pooled at equal concentrations and subjected to four lanes of rapid mode Illumina HiSeq2500 sequencing (paired-end 100bp) at the University of Texas Southwestern Medical Center Genomics and Microarray Core. Illumina HiSeq 2500 gds 302199318 GEO Accession GSM2199318 SRX1840928 GSM2199319 SRP076488 SRS1500109 GSM2199319 RNA-Seq TRANSCRIPTOMIC cDNA Sorted P6 ID4-EGFP+ spermatogonia and subpopulations defined by antibody labeling for TSPAN8, EPHA2, or PVR were used for RNA-seq analysis of transcriptomes. For this purpose, sorted cells were pelleted, counted and subjected to direct cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, Mountain View, CA) per manufacturer recommendations and 9 cycles of amplification. Amplified cDNAs from each sample were purified using Agencourt AMPure XP Pure magnetic beads (Beckman Coulter Life Sciences, Indianapolis, IN) and pre-qualified for library construction using a 2100 Bioanalyzer and High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA). Using 250pg input cDNA, we performed Nextera XT dual-index library preparation with modifications from manufacturer recommendations. Specifically, tagmentation was performed with 2.5ul Tagment DNA buffer, 1.25ul Amplification Tagment Mix, and 1.25ul cDNA for exactly 10 minutes at 55°C, ramp to 10°C, and immediate addition of 1.25ul NT buffer. Additionally, PCR amplification with index primers (separate index per sample) was performed with the entire 6.25ul of Tagmentation reaction mix plus 3.75ul Nextera PCR Mix using the recommended cycling conditions except that the extension time was 60 seconds. Libraries were quantified with Qubit fluorometry (ThermoFisher Scientific) and qualified for fragment size and distribution by 2100 Bioanalyzer with High-sensitivity DNA reagents (522 ± 6 bp). Libraries were pooled at equal concentrations and subjected to four lanes of rapid mode Illumina HiSeq2500 sequencing (paired-end 100bp) at the University of Texas Southwestern Medical Center Genomics and Microarray Core. Illumina HiSeq 2500 gds 302199319 GEO Accession GSM2199319 SRX1840935 GSM2199326 SRP076488 SRS1500116 GSM2199326 RNA-Seq TRANSCRIPTOMIC cDNA Sorted P6 ID4-EGFP+ spermatogonia and subpopulations defined by antibody labeling for TSPAN8, EPHA2, or PVR were used for RNA-seq analysis of transcriptomes. For this purpose, sorted cells were pelleted, counted and subjected to direct cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, Mountain View, CA) per manufacturer recommendations and 9 cycles of amplification. Amplified cDNAs from each sample were purified using Agencourt AMPure XP Pure magnetic beads (Beckman Coulter Life Sciences, Indianapolis, IN) and pre-qualified for library construction using a 2100 Bioanalyzer and High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA). Using 250pg input cDNA, we performed Nextera XT dual-index library preparation with modifications from manufacturer recommendations. Specifically, tagmentation was performed with 2.5ul Tagment DNA buffer, 1.25ul Amplification Tagment Mix, and 1.25ul cDNA for exactly 10 minutes at 55°C, ramp to 10°C, and immediate addition of 1.25ul NT buffer. Additionally, PCR amplification with index primers (separate index per sample) was performed with the entire 6.25ul of Tagmentation reaction mix plus 3.75ul Nextera PCR Mix using the recommended cycling conditions except that the extension time was 60 seconds. Libraries were quantified with Qubit fluorometry (ThermoFisher Scientific) and qualified for fragment size and distribution by 2100 Bioanalyzer with High-sensitivity DNA reagents (522 ± 6 bp). Libraries were pooled at equal concentrations and subjected to four lanes of rapid mode Illumina HiSeq2500 sequencing (paired-end 100bp) at the University of Texas Southwestern Medical Center Genomics and Microarray Core. Illumina HiSeq 2500 gds 302199326 GEO Accession GSM2199326 SRX1840936 GSM2199327 SRP076488 SRS1500117 GSM2199327 RNA-Seq TRANSCRIPTOMIC cDNA Sorted P6 ID4-EGFP+ spermatogonia and subpopulations defined by antibody labeling for TSPAN8, EPHA2, or PVR were used for RNA-seq analysis of transcriptomes. For this purpose, sorted cells were pelleted, counted and subjected to direct cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, Mountain View, CA) per manufacturer recommendations and 9 cycles of amplification. Amplified cDNAs from each sample were purified using Agencourt AMPure XP Pure magnetic beads (Beckman Coulter Life Sciences, Indianapolis, IN) and pre-qualified for library construction using a 2100 Bioanalyzer and High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA). Using 250pg input cDNA, we performed Nextera XT dual-index library preparation with modifications from manufacturer recommendations. Specifically, tagmentation was performed with 2.5ul Tagment DNA buffer, 1.25ul Amplification Tagment Mix, and 1.25ul cDNA for exactly 10 minutes at 55°C, ramp to 10°C, and immediate addition of 1.25ul NT buffer. Additionally, PCR amplification with index primers (separate index per sample) was performed with the entire 6.25ul of Tagmentation reaction mix plus 3.75ul Nextera PCR Mix using the recommended cycling conditions except that the extension time was 60 seconds. Libraries were quantified with Qubit fluorometry (ThermoFisher Scientific) and qualified for fragment size and distribution by 2100 Bioanalyzer with High-sensitivity DNA reagents (522 ± 6 bp). Libraries were pooled at equal concentrations and subjected to four lanes of rapid mode Illumina HiSeq2500 sequencing (paired-end 100bp) at the University of Texas Southwestern Medical Center Genomics and Microarray Core. Illumina HiSeq 2500 gds 302199327 GEO Accession GSM2199327 SRX1840937 GSM2199328 SRP076488 SRS1500118 GSM2199328 RNA-Seq TRANSCRIPTOMIC cDNA Sorted P6 ID4-EGFP+ spermatogonia and subpopulations defined by antibody labeling for TSPAN8, EPHA2, or PVR were used for RNA-seq analysis of transcriptomes. For this purpose, sorted cells were pelleted, counted and subjected to direct cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, Mountain View, CA) per manufacturer recommendations and 9 cycles of amplification. Amplified cDNAs from each sample were purified using Agencourt AMPure XP Pure magnetic beads (Beckman Coulter Life Sciences, Indianapolis, IN) and pre-qualified for library construction using a 2100 Bioanalyzer and High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA). Using 250pg input cDNA, we performed Nextera XT dual-index library preparation with modifications from manufacturer recommendations. Specifically, tagmentation was performed with 2.5ul Tagment DNA buffer, 1.25ul Amplification Tagment Mix, and 1.25ul cDNA for exactly 10 minutes at 55°C, ramp to 10°C, and immediate addition of 1.25ul NT buffer. Additionally, PCR amplification with index primers (separate index per sample) was performed with the entire 6.25ul of Tagmentation reaction mix plus 3.75ul Nextera PCR Mix using the recommended cycling conditions except that the extension time was 60 seconds. Libraries were quantified with Qubit fluorometry (ThermoFisher Scientific) and qualified for fragment size and distribution by 2100 Bioanalyzer with High-sensitivity DNA reagents (522 ± 6 bp). Libraries were pooled at equal concentrations and subjected to four lanes of rapid mode Illumina HiSeq2500 sequencing (paired-end 100bp) at the University of Texas Southwestern Medical Center Genomics and Microarray Core. Illumina HiSeq 2500 gds 302199328 GEO Accession GSM2199328 SRX1840938 GSM2199329 SRP076488 SRS1500119 GSM2199329 RNA-Seq TRANSCRIPTOMIC cDNA Sorted P6 ID4-EGFP+ spermatogonia and subpopulations defined by antibody labeling for TSPAN8, EPHA2, or PVR were used for RNA-seq analysis of transcriptomes. For this purpose, sorted cells were pelleted, counted and subjected to direct cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, Mountain View, CA) per manufacturer recommendations and 9 cycles of amplification. Amplified cDNAs from each sample were purified using Agencourt AMPure XP Pure magnetic beads (Beckman Coulter Life Sciences, Indianapolis, IN) and pre-qualified for library construction using a 2100 Bioanalyzer and High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA). Using 250pg input cDNA, we performed Nextera XT dual-index library preparation with modifications from manufacturer recommendations. Specifically, tagmentation was performed with 2.5ul Tagment DNA buffer, 1.25ul Amplification Tagment Mix, and 1.25ul cDNA for exactly 10 minutes at 55°C, ramp to 10°C, and immediate addition of 1.25ul NT buffer. Additionally, PCR amplification with index primers (separate index per sample) was performed with the entire 6.25ul of Tagmentation reaction mix plus 3.75ul Nextera PCR Mix using the recommended cycling conditions except that the extension time was 60 seconds. Libraries were quantified with Qubit fluorometry (ThermoFisher Scientific) and qualified for fragment size and distribution by 2100 Bioanalyzer with High-sensitivity DNA reagents (522 ± 6 bp). Libraries were pooled at equal concentrations and subjected to four lanes of rapid mode Illumina HiSeq2500 sequencing (paired-end 100bp) at the University of Texas Southwestern Medical Center Genomics and Microarray Core. Illumina HiSeq 2500 gds 302199329 GEO Accession GSM2199329 SRX1840939 GSM2199330 SRP076488 SRS1500120 GSM2199330 RNA-Seq TRANSCRIPTOMIC cDNA Sorted P6 ID4-EGFP+ spermatogonia and subpopulations defined by antibody labeling for TSPAN8, EPHA2, or PVR were used for RNA-seq analysis of transcriptomes. For this purpose, sorted cells were pelleted, counted and subjected to direct cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, Mountain View, CA) per manufacturer recommendations and 9 cycles of amplification. Amplified cDNAs from each sample were purified using Agencourt AMPure XP Pure magnetic beads (Beckman Coulter Life Sciences, Indianapolis, IN) and pre-qualified for library construction using a 2100 Bioanalyzer and High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA). Using 250pg input cDNA, we performed Nextera XT dual-index library preparation with modifications from manufacturer recommendations. Specifically, tagmentation was performed with 2.5ul Tagment DNA buffer, 1.25ul Amplification Tagment Mix, and 1.25ul cDNA for exactly 10 minutes at 55°C, ramp to 10°C, and immediate addition of 1.25ul NT buffer. Additionally, PCR amplification with index primers (separate index per sample) was performed with the entire 6.25ul of Tagmentation reaction mix plus 3.75ul Nextera PCR Mix using the recommended cycling conditions except that the extension time was 60 seconds. Libraries were quantified with Qubit fluorometry (ThermoFisher Scientific) and qualified for fragment size and distribution by 2100 Bioanalyzer with High-sensitivity DNA reagents (522 ± 6 bp). Libraries were pooled at equal concentrations and subjected to four lanes of rapid mode Illumina HiSeq2500 sequencing (paired-end 100bp) at the University of Texas Southwestern Medical Center Genomics and Microarray Core. Illumina HiSeq 2500 gds 302199330 GEO Accession GSM2199330 SRX1840940 GSM2199331 SRP076488 SRS1500121 GSM2199331 RNA-Seq TRANSCRIPTOMIC cDNA Sorted P6 ID4-EGFP+ spermatogonia and subpopulations defined by antibody labeling for TSPAN8, EPHA2, or PVR were used for RNA-seq analysis of transcriptomes. For this purpose, sorted cells were pelleted, counted and subjected to direct cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, Mountain View, CA) per manufacturer recommendations and 9 cycles of amplification. Amplified cDNAs from each sample were purified using Agencourt AMPure XP Pure magnetic beads (Beckman Coulter Life Sciences, Indianapolis, IN) and pre-qualified for library construction using a 2100 Bioanalyzer and High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA). Using 250pg input cDNA, we performed Nextera XT dual-index library preparation with modifications from manufacturer recommendations. Specifically, tagmentation was performed with 2.5ul Tagment DNA buffer, 1.25ul Amplification Tagment Mix, and 1.25ul cDNA for exactly 10 minutes at 55°C, ramp to 10°C, and immediate addition of 1.25ul NT buffer. Additionally, PCR amplification with index primers (separate index per sample) was performed with the entire 6.25ul of Tagmentation reaction mix plus 3.75ul Nextera PCR Mix using the recommended cycling conditions except that the extension time was 60 seconds. Libraries were quantified with Qubit fluorometry (ThermoFisher Scientific) and qualified for fragment size and distribution by 2100 Bioanalyzer with High-sensitivity DNA reagents (522 ± 6 bp). Libraries were pooled at equal concentrations and subjected to four lanes of rapid mode Illumina HiSeq2500 sequencing (paired-end 100bp) at the University of Texas Southwestern Medical Center Genomics and Microarray Core. Illumina HiSeq 2500 gds 302199331 GEO Accession GSM2199331