SRX1997749 micPCR_SMC_0_replicate1 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_0_replicate1 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997750 micPCR_SMC_0_replicate2 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_0_replicate2 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997751 micPCR_SMC_0_replicate3 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_0_replicate3 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997752 micPCR_SMC_2.5_replicate1 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_2.5_replicate1 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997753 micPCR_SMC_2.5_replicate2 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_2.5_replicate2 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997754 micPCR_SMC_2.5_replicate3 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_2.5_replicate3 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997755 micPCR_SMC_25_replicate1 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_25_replicate1 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997756 micPCR_SMC_25_replicate2 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_25_replicate2 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997757 micPCR_SMC_25_replicate3 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_25_replicate3 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997758 micPCR_SMC_250_replicate1 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_250_replicate1 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997759 micPCR_SMC_250_replicate2 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_250_replicate2 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997760 micPCR_SMC_250_replicate3 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_250_replicate3 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997761 micPCR_SMC_2500_replicate1 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_2500_replicate1 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997762 micPCR_SMC_2500_replicate2 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_2500_replicate2 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997763 micPCR_SMC_2500_replicate3 SRP076831 PRJNA325566 "The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon)." SRS1518581 SAMN05240950 micPCR_SMC_2500_replicate3 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997764 PCR_SMC_0_replicate1 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_0_replicate1 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997765 PCR_SMC_0_replicate2 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_0_replicate2 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997766 PCR_SMC_0_replicate3 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_0_replicate3 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997767 PCR_SMC_2.5_replicate1 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_2.5_replicate1 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997768 PCR_SMC_2.5_replicate2 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_2.5_replicate2 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997769 PCR_SMC_2.5_replicate3 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_2.5_replicate3 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997770 PCR_SMC_25_replicate1 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_25_replicate1 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997771 PCR_SMC_25_replicate2 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_25_replicate2 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997772 PCR_SMC_25_replicate3 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_25_replicate3 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997773 PCR_SMC_250_replicate1 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_250_replicate1 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997774 PCR_SMC_250_replicate2 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_250_replicate2 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997775 PCR_SMC_250_replicate3 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_250_replicate3 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997776 PCR_SMC_2500_replicate1 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_2500_replicate1 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997777 PCR_SMC_2500_replicate2 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_2500_replicate2 AMPLICON METAGENOMIC PCR 454 GS Junior SRX1997778 PCR_SMC_2500_replicate3 SRP076831 PRJNA325566 "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs." SRS1518581 SAMN05240950 PCR_SMC_2500_replicate3 AMPLICON METAGENOMIC PCR 454 GS Junior SRX2007323 micPCR_Skinswab01_repicate1 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606119 SAMN05506988 micPCR_Skinswab01_repicate1 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007324 micPCR_Skinswab01_repicate2 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606119 micPCR_Skinswab01_repicate2 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007325 micPCR_Skinswab01_repicate3 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606119 micPCR_Skinswab01_repicate3 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007347 micPCR_Skinswab02_repicate1 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606141 SAMN05506986 micPCR_Skinswab02_repicate1 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007356 micPCR_Skinswab02_repicate2 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606141 SAMN05506986 micPCR_Skinswab02_repicate2 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007360 micPCR_Skinswab02_repicate3 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606141 micPCR_Skinswab02_repicate3 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007365 micPCR_Skinswab03_repicate1 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606157 SAMN05506991 micPCR_Skinswab03_repicate1 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007366 micPCR_Skinswab03_repicate2 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606157 SAMN05506991 micPCR_Skinswab03_repicate2 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007367 micPCR_Skinswab03_repicate3 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606157 micPCR_Skinswab03_repicate3 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007369 micPCR_Skinswab04_repicate1 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606159 SAMN05506990 micPCR_Skinswab04_repicate1 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007370 micPCR_Skinswab04_repicate2 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606159 micPCR_Skinswab04_repicate2 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007371 micPCR_Skinswab04_repicate3 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606159 micPCR_Skinswab04_repicate3 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007376 micPCR_SkinswabNEC_repicate1 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606162 SAMN05506992 micPCR_SkinswabNEC_repicate1 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007377 micPCR_SkinswabNEC_repicate2 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606162 SAMN05506992 micPCR_SkinswabNEC_repicate2 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior SRX2007378 micPCR_SkinswabNEC_repicate3 SRP076831 The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a micPCR was again used, but to amplify micPCR amplicons obtained from the first step micPCR. The second step micPCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs. For both amplification steps, water-in-oil emulsions were prepared using the Micellula DNA Emulsion Kit (Roboklon). SRS1606162 micPCR_SkinswabNEC_repicate3 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior