SRX1842836 GSM2199533 SRP076520 SRS1501742 GSM2199533 RNA-Seq TRANSCRIPTOMIC cDNA Rats were euthanized under anesthesia according to approved IACUC protocols. Hypothalami were rapidly dissected from brain tissue samples on ice and immediately snap frozen in liquid nitrogen. RNA extraction (N=4 biological replicates of each genotype, male Mecp2ZFN/y and male wild-type littermate rats, 6 weeks of life) was performed using TRizol reagent (ThermoFisher Scientific) and further purified with on-column DNase treatment using the RNeasy Mini Kit (Qiagen) following the manufacturers’ instructions. Ten µg of RNA was prepared for paired-end RNA-Sequencing using Illumina HiSeq 2500. The Baylor College of Medicine Genomic and RNA Profiling Core first conducted Sample Quality checks of the RNA using the NanoDrop ND-1000 spectrophotometer and Agilent Bioanalyzer 2100 using the Nano chip.  The Illumina EpiCentre Ribo-Zero rRNA Removal Kit was used to reduce the ribosomal RNA content.  The rRNA-reduced sample was then carried into the Illumina TruSeq RNA library preparation protocol. Illumina HiSeq 2500 gds 302199533 GEO Accession GSM2199533 SRX1842837 GSM2199534 SRP076520 SRS1501744 GSM2199534 RNA-Seq TRANSCRIPTOMIC cDNA Rats were euthanized under anesthesia according to approved IACUC protocols. Hypothalami were rapidly dissected from brain tissue samples on ice and immediately snap frozen in liquid nitrogen. RNA extraction (N=4 biological replicates of each genotype, male Mecp2ZFN/y and male wild-type littermate rats, 6 weeks of life) was performed using TRizol reagent (ThermoFisher Scientific) and further purified with on-column DNase treatment using the RNeasy Mini Kit (Qiagen) following the manufacturers’ instructions. Ten µg of RNA was prepared for paired-end RNA-Sequencing using Illumina HiSeq 2500. The Baylor College of Medicine Genomic and RNA Profiling Core first conducted Sample Quality checks of the RNA using the NanoDrop ND-1000 spectrophotometer and Agilent Bioanalyzer 2100 using the Nano chip.  The Illumina EpiCentre Ribo-Zero rRNA Removal Kit was used to reduce the ribosomal RNA content.  The rRNA-reduced sample was then carried into the Illumina TruSeq RNA library preparation protocol. Illumina HiSeq 2500 gds 302199534 GEO Accession GSM2199534 SRX1842838 GSM2199535 SRP076520 SRS1501743 GSM2199535 RNA-Seq TRANSCRIPTOMIC cDNA Rats were euthanized under anesthesia according to approved IACUC protocols. Hypothalami were rapidly dissected from brain tissue samples on ice and immediately snap frozen in liquid nitrogen. RNA extraction (N=4 biological replicates of each genotype, male Mecp2ZFN/y and male wild-type littermate rats, 6 weeks of life) was performed using TRizol reagent (ThermoFisher Scientific) and further purified with on-column DNase treatment using the RNeasy Mini Kit (Qiagen) following the manufacturers’ instructions. Ten µg of RNA was prepared for paired-end RNA-Sequencing using Illumina HiSeq 2500. The Baylor College of Medicine Genomic and RNA Profiling Core first conducted Sample Quality checks of the RNA using the NanoDrop ND-1000 spectrophotometer and Agilent Bioanalyzer 2100 using the Nano chip.  The Illumina EpiCentre Ribo-Zero rRNA Removal Kit was used to reduce the ribosomal RNA content.  The rRNA-reduced sample was then carried into the Illumina TruSeq RNA library preparation protocol. Illumina HiSeq 2500 gds 302199535 GEO Accession GSM2199535 SRX1842839 GSM2199536 SRP076520 SRS1501745 GSM2199536 RNA-Seq TRANSCRIPTOMIC cDNA Rats were euthanized under anesthesia according to approved IACUC protocols. Hypothalami were rapidly dissected from brain tissue samples on ice and immediately snap frozen in liquid nitrogen. RNA extraction (N=4 biological replicates of each genotype, male Mecp2ZFN/y and male wild-type littermate rats, 6 weeks of life) was performed using TRizol reagent (ThermoFisher Scientific) and further purified with on-column DNase treatment using the RNeasy Mini Kit (Qiagen) following the manufacturers’ instructions. Ten µg of RNA was prepared for paired-end RNA-Sequencing using Illumina HiSeq 2500. The Baylor College of Medicine Genomic and RNA Profiling Core first conducted Sample Quality checks of the RNA using the NanoDrop ND-1000 spectrophotometer and Agilent Bioanalyzer 2100 using the Nano chip.  The Illumina EpiCentre Ribo-Zero rRNA Removal Kit was used to reduce the ribosomal RNA content.  The rRNA-reduced sample was then carried into the Illumina TruSeq RNA library preparation protocol. Illumina HiSeq 2500 gds 302199536 GEO Accession GSM2199536 SRX1842840 GSM2199537 SRP076520 SRS1501746 GSM2199537 RNA-Seq TRANSCRIPTOMIC cDNA Rats were euthanized under anesthesia according to approved IACUC protocols. Hypothalami were rapidly dissected from brain tissue samples on ice and immediately snap frozen in liquid nitrogen. RNA extraction (N=4 biological replicates of each genotype, male Mecp2ZFN/y and male wild-type littermate rats, 6 weeks of life) was performed using TRizol reagent (ThermoFisher Scientific) and further purified with on-column DNase treatment using the RNeasy Mini Kit (Qiagen) following the manufacturers’ instructions. Ten µg of RNA was prepared for paired-end RNA-Sequencing using Illumina HiSeq 2500. The Baylor College of Medicine Genomic and RNA Profiling Core first conducted Sample Quality checks of the RNA using the NanoDrop ND-1000 spectrophotometer and Agilent Bioanalyzer 2100 using the Nano chip.  The Illumina EpiCentre Ribo-Zero rRNA Removal Kit was used to reduce the ribosomal RNA content.  The rRNA-reduced sample was then carried into the Illumina TruSeq RNA library preparation protocol. Illumina HiSeq 2500 gds 302199537 GEO Accession GSM2199537 SRX1842841 GSM2199538 SRP076520 SRS1501747 GSM2199538 RNA-Seq TRANSCRIPTOMIC cDNA Rats were euthanized under anesthesia according to approved IACUC protocols. Hypothalami were rapidly dissected from brain tissue samples on ice and immediately snap frozen in liquid nitrogen. RNA extraction (N=4 biological replicates of each genotype, male Mecp2ZFN/y and male wild-type littermate rats, 6 weeks of life) was performed using TRizol reagent (ThermoFisher Scientific) and further purified with on-column DNase treatment using the RNeasy Mini Kit (Qiagen) following the manufacturers’ instructions. Ten µg of RNA was prepared for paired-end RNA-Sequencing using Illumina HiSeq 2500. The Baylor College of Medicine Genomic and RNA Profiling Core first conducted Sample Quality checks of the RNA using the NanoDrop ND-1000 spectrophotometer and Agilent Bioanalyzer 2100 using the Nano chip.  The Illumina EpiCentre Ribo-Zero rRNA Removal Kit was used to reduce the ribosomal RNA content.  The rRNA-reduced sample was then carried into the Illumina TruSeq RNA library preparation protocol. Illumina HiSeq 2500 gds 302199538 GEO Accession GSM2199538 SRX1842842 GSM2199539 SRP076520 SRS1501748 GSM2199539 RNA-Seq TRANSCRIPTOMIC cDNA Rats were euthanized under anesthesia according to approved IACUC protocols. Hypothalami were rapidly dissected from brain tissue samples on ice and immediately snap frozen in liquid nitrogen. RNA extraction (N=4 biological replicates of each genotype, male Mecp2ZFN/y and male wild-type littermate rats, 6 weeks of life) was performed using TRizol reagent (ThermoFisher Scientific) and further purified with on-column DNase treatment using the RNeasy Mini Kit (Qiagen) following the manufacturers’ instructions. Ten µg of RNA was prepared for paired-end RNA-Sequencing using Illumina HiSeq 2500. The Baylor College of Medicine Genomic and RNA Profiling Core first conducted Sample Quality checks of the RNA using the NanoDrop ND-1000 spectrophotometer and Agilent Bioanalyzer 2100 using the Nano chip.  The Illumina EpiCentre Ribo-Zero rRNA Removal Kit was used to reduce the ribosomal RNA content.  The rRNA-reduced sample was then carried into the Illumina TruSeq RNA library preparation protocol. Illumina HiSeq 2500 gds 302199539 GEO Accession GSM2199539 SRX1842843 GSM2199540 SRP076520 SRS1501749 GSM2199540 RNA-Seq TRANSCRIPTOMIC cDNA Rats were euthanized under anesthesia according to approved IACUC protocols. Hypothalami were rapidly dissected from brain tissue samples on ice and immediately snap frozen in liquid nitrogen. RNA extraction (N=4 biological replicates of each genotype, male Mecp2ZFN/y and male wild-type littermate rats, 6 weeks of life) was performed using TRizol reagent (ThermoFisher Scientific) and further purified with on-column DNase treatment using the RNeasy Mini Kit (Qiagen) following the manufacturers’ instructions. Ten µg of RNA was prepared for paired-end RNA-Sequencing using Illumina HiSeq 2500. The Baylor College of Medicine Genomic and RNA Profiling Core first conducted Sample Quality checks of the RNA using the NanoDrop ND-1000 spectrophotometer and Agilent Bioanalyzer 2100 using the Nano chip.  The Illumina EpiCentre Ribo-Zero rRNA Removal Kit was used to reduce the ribosomal RNA content.  The rRNA-reduced sample was then carried into the Illumina TruSeq RNA library preparation protocol. Illumina HiSeq 2500 gds 302199540 GEO Accession GSM2199540